BRENDA - Enzyme Database show

Cloning and characterization of aspartate-beta-semialdehyde dehydrogenase from Mycobacterium tuberculosis H37 Rv

Shafiani, S.; Sharma, P.; Vohra, R.M.; Tewari, R.; J. Appl. Microbiol. 98, 832-838 (2005)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
1.2.1.11
medicine
enzyme is a validated drug target
Mycobacterium tuberculosis
1.2.1.11
pharmacology
inhibitor design from enzyme three-dimensional structure
Mycobacterium tuberculosis
Cloned(Commentary)
EC Number
Commentary
Organism
1.2.1.11
gene asd, subcloning in Escherichia coli strain JM109, DNA sequence determination and analysis, optimization of functional overexpression in Escherichia coli strain M15 as His-tagged protein
Mycobacterium tuberculosis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.1.11
0.065
-
NADP+
recombinant enzyme, pH 9.0, 30°C
Mycobacterium tuberculosis
1.2.1.11
0.955
-
L-aspartate-4-semialdehyde
recombinant enzyme, pH 9.0, 30°C
Mycobacterium tuberculosis
1.2.1.11
11.39
-
phosphate
recombinant enzyme, pH 9.0, 30°C
Mycobacterium tuberculosis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.1.11
37000
-
x * 37000, about, recombinant enzyme, SDS-PAGE
Mycobacterium tuberculosis
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.1.11
L-4-aspartyl phosphate + NADPH
Mycobacterium tuberculosis
physiological forward reaction direction, key enzyme in diaminopimelic acid biosynthetic pathway
L-aspartate-4-semialdehyde + phosphate + NADP+
-
-
?
1.2.1.11
L-4-aspartyl phosphate + NADPH
Mycobacterium tuberculosis H37Rv
physiological forward reaction direction, key enzyme in diaminopimelic acid biosynthetic pathway
L-aspartate-4-semialdehyde + phosphate + NADP+
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.1.11
Mycobacterium tuberculosis
P9WNX5
strain H37 Rv, gene asd
-
1.2.1.11
Mycobacterium tuberculosis H37Rv
P9WNX5
strain H37 Rv, gene asd
-
Purification (Commentary)
EC Number
Commentary
Organism
1.2.1.11
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography to 96% purity
Mycobacterium tuberculosis
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.2.1.11
additional information
-
purified recombinant enzyme
Mycobacterium tuberculosis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.11
L-4-aspartyl phosphate + NADPH
physiological forward reaction direction, key enzyme in diaminopimelic acid biosynthetic pathway
655821
Mycobacterium tuberculosis
L-aspartate-4-semialdehyde + phosphate + NADP+
-
-
-
?
1.2.1.11
L-4-aspartyl phosphate + NADPH
physiological forward reaction direction, key enzyme in diaminopimelic acid biosynthetic pathway
655821
Mycobacterium tuberculosis H37Rv
L-aspartate-4-semialdehyde + phosphate + NADP+
-
-
-
?
1.2.1.11
L-aspartate-4-semialdehyde + phosphate + NADP+
-
655821
Mycobacterium tuberculosis
L-4-aspartyl phosphate + NADPH
-
-
-
r
1.2.1.11
L-aspartate-4-semialdehyde + phosphate + NADP+
-
655821
Mycobacterium tuberculosis H37Rv
L-4-aspartyl phosphate + NADPH
-
-
-
r
Subunits
EC Number
Subunits
Commentary
Organism
1.2.1.11
?
x * 37000, about, recombinant enzyme, SDS-PAGE
Mycobacterium tuberculosis
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.2.1.11
30
-
assay at
Mycobacterium tuberculosis
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.2.1.11
8.49
-
NADP+
recombinant enzyme, pH 9.0, 30°C
Mycobacterium tuberculosis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.1.11
9
-
assay at
Mycobacterium tuberculosis
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.11
NADP+
-
Mycobacterium tuberculosis
1.2.1.11
NADPH
-
Mycobacterium tuberculosis
Application (protein specific)
EC Number
Application
Commentary
Organism
1.2.1.11
medicine
enzyme is a validated drug target
Mycobacterium tuberculosis
1.2.1.11
pharmacology
inhibitor design from enzyme three-dimensional structure
Mycobacterium tuberculosis
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.1.11
gene asd, subcloning in Escherichia coli strain JM109, DNA sequence determination and analysis, optimization of functional overexpression in Escherichia coli strain M15 as His-tagged protein
Mycobacterium tuberculosis
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.11
NADP+
-
Mycobacterium tuberculosis
1.2.1.11
NADPH
-
Mycobacterium tuberculosis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.1.11
0.065
-
NADP+
recombinant enzyme, pH 9.0, 30°C
Mycobacterium tuberculosis
1.2.1.11
0.955
-
L-aspartate-4-semialdehyde
recombinant enzyme, pH 9.0, 30°C
Mycobacterium tuberculosis
1.2.1.11
11.39
-
phosphate
recombinant enzyme, pH 9.0, 30°C
Mycobacterium tuberculosis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.1.11
37000
-
x * 37000, about, recombinant enzyme, SDS-PAGE
Mycobacterium tuberculosis
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.1.11
L-4-aspartyl phosphate + NADPH
Mycobacterium tuberculosis
physiological forward reaction direction, key enzyme in diaminopimelic acid biosynthetic pathway
L-aspartate-4-semialdehyde + phosphate + NADP+
-
-
?
1.2.1.11
L-4-aspartyl phosphate + NADPH
Mycobacterium tuberculosis H37Rv
physiological forward reaction direction, key enzyme in diaminopimelic acid biosynthetic pathway
L-aspartate-4-semialdehyde + phosphate + NADP+
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.1.11
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography to 96% purity
Mycobacterium tuberculosis
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.2.1.11
additional information
-
purified recombinant enzyme
Mycobacterium tuberculosis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.11
L-4-aspartyl phosphate + NADPH
physiological forward reaction direction, key enzyme in diaminopimelic acid biosynthetic pathway
655821
Mycobacterium tuberculosis
L-aspartate-4-semialdehyde + phosphate + NADP+
-
-
-
?
1.2.1.11
L-4-aspartyl phosphate + NADPH
physiological forward reaction direction, key enzyme in diaminopimelic acid biosynthetic pathway
655821
Mycobacterium tuberculosis H37Rv
L-aspartate-4-semialdehyde + phosphate + NADP+
-
-
-
?
1.2.1.11
L-aspartate-4-semialdehyde + phosphate + NADP+
-
655821
Mycobacterium tuberculosis
L-4-aspartyl phosphate + NADPH
-
-
-
r
1.2.1.11
L-aspartate-4-semialdehyde + phosphate + NADP+
-
655821
Mycobacterium tuberculosis H37Rv
L-4-aspartyl phosphate + NADPH
-
-
-
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.2.1.11
?
x * 37000, about, recombinant enzyme, SDS-PAGE
Mycobacterium tuberculosis
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.2.1.11
30
-
assay at
Mycobacterium tuberculosis
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.2.1.11
8.49
-
NADP+
recombinant enzyme, pH 9.0, 30°C
Mycobacterium tuberculosis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.1.11
9
-
assay at
Mycobacterium tuberculosis