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Literature summary extracted from

  • Tan, X.; Loke, H.K.; Fitch, S.; Lindahl, P.A.
    The tunnel of acetyl-coenzyme A synthase/carbon monoxide dehydrogenase regulates delivery of CO to the active site (2005), J. Am. Chem. Soc., 127, 5833-5839.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.7.4 Nickel Ni-activated alpha subunit rapidly and reversibly accepts a methyl group Moorella thermoacetica

Protein Variants

EC Number Protein Variants Comment Organism
1.2.7.4 A110C mutation within tunnel region of alpha subunit, absence of strong cooperative inhibition of CO, no synthesis of acetyl-CoA Moorella thermoacetica
1.2.7.4 A222L mutation within tunnel region of alpha subunit, absence of strong cooperative inhibition of CO, no synthesis of acetyl-CoA Moorella thermoacetica
1.2.7.4 A265M mutation within tunnel region of alpha subunit, absence of strong cooperative inhibition of CO, little synthesis of acetyl-CoA Moorella thermoacetica
2.3.1.169 A110C absence of strong cooperative inhibition of CO which characterizes wild-type enzyme Moorella thermoacetica
2.3.1.169 A222L the bifunctional mutant enzyme is not able to synthesize acetyl-CoA using CO2 as substrate Moorella thermoacetica
2.3.1.169 A265M absence of strong cooperative inhibition of CO which characterizes wild-type enzyme Moorella thermoacetica

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.7.4 CO weak, isolated alpha subunit Moorella thermoacetica
2.3.1.169 CO the complete alpha2beta2 enzyme exhibits strong cooperative inhibition, isolated alpha is weakly inhibited, apparently by a single CO with Ki = 1.5 mM. Absence of strong cooperative inhibition of CO in mutant enzymes A265M and AA110C Moorella thermoacetica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.7.4 0.03
-
 CO 25°C, isolated alpha subunit Moorella thermoacetica

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.4 Moorella thermoacetica
-
 bifunctional acetyl-coenzyme A synthase/carbon monoxide dehydrogenase, isolated alpha subunit
-
2.3.1.169 Moorella thermoacetica
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.7.4 100
-
 25°C, mutant A265M Moorella thermoacetica
1.2.7.4 200
-
 25°C, wild type Moorella thermoacetica
1.2.7.4 280
-
 25°C, mutant A110C Moorella thermoacetica
1.2.7.4 300
-
 25°C, mutant A222L Moorella thermoacetica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.4 CO + H2O + acceptor
-
 Moorella thermoacetica CO2 + reduced acceptor
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.169 acetyl-coenzyme A synthase/carbon monoxide dehydrogenase
-
 Moorella thermoacetica
2.3.1.169 ACS/CODH
-
 Moorella thermoacetica

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.7.4 11
-
 CO 25°C, isolated alpha subunit Moorella thermoacetica

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.2.7.4 1.5
-
 CO 25°C Moorella thermoacetica
2.3.1.169 1.5
-
 CO the complete alpha2beta2 enzyme exhibits strong cooperative inhibition, isolated alpha is weakly inhibited, apparently by a single CO with Ki = 1.5 mM Moorella thermoacetica