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Literature summary extracted from

  • Yee, D.J.; Balsanek, V.; Sames, D.
    New tools for molecular imaging of redox metabolism: development of a fluorogenic probe for 3a-hydroxysteroid dehydrogenases (2004), J. Am. Chem. Soc., 126, 2282-2283.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.50 0.0025
-
 oxidized 8-acetyl-2,3,5,6-tetrahydro-1H,4H-11-oxa-3a-azabenzo[de]anthracen-10-one isozyme AKR1C3 Homo sapiens
1.1.1.50 0.026
-
 5alpha-dihydroprogesterone isozyme AKR1C3 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.50 5alpha-dihydroprogesterone + 2 NAD(P)H + 2 H+ Homo sapiens
-
 pregnan-3alpha,20beta-diol + 2 NAD(P)+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.50 Comamonas testosteroni
-
-
-
1.1.1.50 Homo sapiens
-
 type 2 isozyme AKR1C3
-
1.1.1.50 Rattus norvegicus
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.50 additional information
-
 developemnt of noninvasive method for visualization and quantification of the reaction, development of selective fluorogenic substrate compounds, overview Rattus norvegicus
1.1.1.50 additional information
-
 developemnt of noninvasive method for visualization and quantification of the reaction, development of selective fluorogenic substrate compounds, overview Comamonas testosteroni
1.1.1.50 additional information
-
 development of noninvasive method for visualization and quantification of the reaction, development of selective fluorogenic substrate compounds, overview Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.50 5alpha-dihydroprogesterone + 2 NAD(P)H + 2 H+
-
 Homo sapiens pregnan-3alpha,20beta-diol + 2 NAD(P)+
-
 ?
1.1.1.50 oxidized 8-acetyl-2,3,5,6-tetrahydro-1H,4H-11-oxa-3a-azabenzo[de]anthracen-10-one + NAD(P)H synthetic compound with fluorophore core Homo sapiens reduced 8-acetyl-2,3,5,6-tetrahydro-1H,4H-11-oxa-3a-azabenzo[de]anthracen-10-one + NAD(P)+
-
 r
1.1.1.50 oxidized 8-acetyl-2,3,5,6-tetrahydro-1H,4H-11-oxa-3a-azabenzo[de]anthracen-10-one + NAD(P)H synthetic compound with fluorophore core Rattus norvegicus reduced 8-acetyl-2,3,5,6-tetrahydro-1H,4H-11-oxa-3a-azabenzo[de]anthracen-10-one + NAD(P)+
-
 r
1.1.1.50 oxidized 8-acetyl-2,3,5,6-tetrahydro-1H,4H-11-oxa-3a-azabenzo[de]anthracen-10-one + NAD(P)H synthetic compound with fluorophore core Comamonas testosteroni reduced 8-acetyl-2,3,5,6-tetrahydro-1H,4H-11-oxa-3a-azabenzo[de]anthracen-10-one + NAD(P)+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.50 3alpha-hydroxysteroid dehydrogenase
-
 Homo sapiens
1.1.1.50 3alpha-hydroxysteroid dehydrogenase
-
 Rattus norvegicus
1.1.1.50 3alpha-hydroxysteroid dehydrogenase
-
 Comamonas testosteroni
1.1.1.50 3alphaHSD
-
 Homo sapiens
1.1.1.50 3HSD
-
 Rattus norvegicus
1.1.1.50 3HSD
-
 Comamonas testosteroni
1.1.1.50 AKR1C3
-
 Homo sapiens
1.1.1.50 More enzyme belongs to the aldo-keto reductase AKR superfamily Homo sapiens
1.1.1.50 PT3HSD
-
 Comamonas testosteroni

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.50 0.004
-
 5alpha-dihydroprogesterone isozyme AKR1C3 Homo sapiens
1.1.1.50 0.14
-
 oxidized 8-acetyl-2,3,5,6-tetrahydro-1H,4H-11-oxa-3a-azabenzo[de]anthracen-10-one isozyme AKR1C3 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.50 NAD(P)+
-
 Homo sapiens
1.1.1.50 NAD(P)+
-
 Rattus norvegicus
1.1.1.50 NAD(P)+
-
 Comamonas testosteroni
1.1.1.50 NAD(P)H
-
 Homo sapiens
1.1.1.50 NAD(P)H
-
 Rattus norvegicus
1.1.1.50 NAD(P)H
-
 Comamonas testosteroni