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Literature summary extracted from

  • Yang, L.; Ning, Z.S.; Shi, C.Z.; Chang, Z.Y.; Huan, L.Y.
    Purification and characterization of an isoflavone-conjugates-hydrolyzing beta-glucosidase from endophytic bacterium (2004), J. Agric. Food Chem., 52, 1940-1944.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.21 beta-mercaptoethanol 1 mM, slight activation Pseudomonas sp.
3.2.1.21 dithioerythritol 1 mM, slight activation Pseudomonas sp.
3.2.1.21 dithiothreitol 1 mM, slight activation Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 AgNO3 1 mM, 88% inhibition Pseudomonas sp.
3.2.1.21 D-glucose
-
Pseudomonas sp.
3.2.1.21 glucono-delta-lactone
-
Pseudomonas sp.
3.2.1.21 HgCl2 1 mM, 94% inhibition Pseudomonas sp.
3.2.1.21 iodoacetic acid 1 mM, 92% inhibition Pseudomonas sp.
3.2.1.21 N-ethylmaleimide 1 mM, complete inhibition Pseudomonas sp.
3.2.1.21 p-chloromercuribenzoate 1 mM, complete inhibition Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 32000
-
gel filtration Pseudomonas sp.
3.2.1.21 33000
-
1 * 33000, SDS-PAGE Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Pseudomonas sp.
-
-
-
3.2.1.21 Pseudomonas sp. ZD-8
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 ammonium sulfate, Sephadex G-100, DEAE-Sepharose,CL-6B, DEAE-Sephacel Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.21 1485
-
-
Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 apigenin-7-beta-D-glucopyranoside + H2O
-
Pseudomonas sp. apigenin + beta-D-glucose
-
?
3.2.1.21 apigenin-7-beta-D-glucopyranoside + H2O
-
Pseudomonas sp. ZD-8 apigenin + beta-D-glucose
-
?
3.2.1.21 arbutin + H2O
-
Pseudomonas sp. beta-D-glucose + 4-hydroxyphenol
-
?
3.2.1.21 arbutin + H2O
-
Pseudomonas sp. ZD-8 beta-D-glucose + 4-hydroxyphenol
-
?
3.2.1.21 daidzein 7-O-(6-O-malonyl-beta-D-glucoside) + H2O
-
Pseudomonas sp. daidzein + 6-O-malonyl-beta-D-glucopyranose
-
?
3.2.1.21 daidzein 7-O-(6-O-malonyl-beta-D-glucoside) + H2O
-
Pseudomonas sp. ZD-8 daidzein + 6-O-malonyl-beta-D-glucopyranose
-
?
3.2.1.21 daidzein-7-O-beta-D-glucopyranoside + H2O
-
Pseudomonas sp. daidzein + beta-D-glucose
-
?
3.2.1.21 daidzein-7-O-beta-D-glucopyranoside + H2O
-
Pseudomonas sp. ZD-8 daidzein + beta-D-glucose
-
?
3.2.1.21 esculin + H2O
-
Pseudomonas sp. beta-D-glucose + esculetin
-
?
3.2.1.21 esculin + H2O
-
Pseudomonas sp. ZD-8 beta-D-glucose + esculetin
-
?
3.2.1.21 genistein-7-O-beta-D-glucopyranoside + H2O
-
Pseudomonas sp. genistein + beta-D-glucose
-
?
3.2.1.21 genistein-7-O-beta-D-glucosyl-6''-O-malonate + H2O
-
Pseudomonas sp. genistein + 6-O-malonyl-beta-D-glucose
-
?
3.2.1.21 p-nitrophenyl-beta-D-galactopyranoside + H2O
-
Pseudomonas sp. p-nitrophenol + beta-D-galactose
-
?
3.2.1.21 p-nitrophenyl-beta-D-glucopyranoside + H2O
-
Pseudomonas sp. p-nitrophenol + beta-D-glucose
-
?
3.2.1.21 p-nitrophenyl-beta-D-mannopyranoside + H2O
-
Pseudomonas sp. p-nitrophenol + beta-D-mannose
-
?
3.2.1.21 salicin + H2O
-
Pseudomonas sp. beta-D-glucose + salicylic alcohol
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.21 monomer 1 * 33000, SDS-PAGE Pseudomonas sp.

Synonyms

EC Number Synonyms Comment Organism
3.2.1.21 ICHG
-
Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 40
-
-
Pseudomonas sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.21 20 70 approx. 60% of maximal activity at 20°C, approx. 10% of maximal activity at 65% Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.21 30
-
no loss of activity after 30 min Pseudomonas sp.
3.2.1.21 40
-
approx. 20% loss of activity after 30 min Pseudomonas sp.
3.2.1.21 50
-
approx. 56% loss of activity after 30 min Pseudomonas sp.
3.2.1.21 60
-
approx. 95% loss of activity after 30 min Pseudomonas sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.21 0.32
-
p-nitrophenyl-beta-D-mannopyranoside 40°C, pH 6.0 Pseudomonas sp.
3.2.1.21 0.62
-
p-nitrophenyl-beta-D-galactopyranoside 40°C, pH 6.0 Pseudomonas sp.
3.2.1.21 0.87
-
p-nitrophenyl-beta-D-glucopyranoside 40°C, pH 6.0 Pseudomonas sp.
3.2.1.21 1.06
-
arbutin 40°C, pH 6.0 Pseudomonas sp.
3.2.1.21 1.32
-
Salicin 40°C, pH 6.0 Pseudomonas sp.
3.2.1.21 1.57
-
esculin 40°C, pH 6.0 Pseudomonas sp.
3.2.1.21 2.39
-
daidzein 7-O-(6-O-malonyl-beta-D-glucoside) 40°C, pH 6.0 Pseudomonas sp.
3.2.1.21 2.68
-
genistein-7-beta-D-glucosyl-6''-O-malonate 40°C, pH 6.0 Pseudomonas sp.
3.2.1.21 3.92
-
apigenin-7-O-beta-D-glucopyranoside 40°C, pH 6.0 Pseudomonas sp.
3.2.1.21 5.12
-
daidzein-7-beta-D-glucopyranoside 40°C, pH 6.0 Pseudomonas sp.
3.2.1.21 5.31
-
genistein-7-beta-D-glucopyranoside 40°C, pH 6.0 Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 6
-
-
Pseudomonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.21 3.5 8.5 approx. 60% of maxim activity at pH 4.0, approx. 70% of maximal activity at pH 8.0 Pseudomonas sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.21 3.5 8.5 approx. 30% loss of activity after 2 h at pH 3.5 and 30°C, approx. 20% loss of activity after 2 h at pH 8.5 and 30°C Pseudomonas sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.21 23
-
glucono-delta-lactone 40°C, pH 6.0, vs. genistein-7-O-beta-D-glucopyranoside Pseudomonas sp.
3.2.1.21 84
-
D-glucose 40°C, pH 6.0, vs. genistein-7-O-beta-D-glucopyranoside Pseudomonas sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.21 Pseudomonas sp. isoelectric focusing
-
4.9