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Literature summary extracted from

  • King, M.R.; White, B.A.; Blaschek, H.P.; Chassy, B.M.; Mackie, R.I.; Cann, I.K.O.
    Purification and characterization of a thermostable alpha-galactosidase from Thermoanaerobacterium polysaccharolyticum (2002), J. Agric. Food Chem., 50, 5676-5682.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.22 nutrition food ingredients containing alpha-1,6-galactoside bond elicit gastrointestinal disturbances in monogastric animals, including humans. Pretreatment of such ingredients with alpha-galactosidase has the potential to alleviate this condition Caldanaerobius polysaccharolyticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.22 CaCl2 10 mM, 19.9% inhibition Caldanaerobius polysaccharolyticus
3.2.1.22 CdSO4 0.25 mM, 99.9% inhibition Caldanaerobius polysaccharolyticus
3.2.1.22 CoSO4 0.25 mM, 8.3% inhibition Caldanaerobius polysaccharolyticus
3.2.1.22 CuCl2 1.0 mM, 30.8% inhibition Caldanaerobius polysaccharolyticus
3.2.1.22 FeCl3 1.0 mM, 46.2% inhibition Caldanaerobius polysaccharolyticus
3.2.1.22 FeSO4 1.0 mM, 50.7% inhibition Caldanaerobius polysaccharolyticus
3.2.1.22 galactose
-
 Caldanaerobius polysaccharolyticus
3.2.1.22 HgCl2 1.0 mM, 88.2% inhibition Caldanaerobius polysaccharolyticus
3.2.1.22 MgCl2 10 mM, 16.4% inhibition Caldanaerobius polysaccharolyticus
3.2.1.22 MnCl2 1.0 mM, 18.4% inhibition Caldanaerobius polysaccharolyticus
3.2.1.22 NEM 10 mM, 12.9% inhibition Caldanaerobius polysaccharolyticus
3.2.1.22 NiCl2 1.0 mM, 21.7% inhibition Caldanaerobius polysaccharolyticus
3.2.1.22 p-hydroxymercuribenzoate 0.25 mM, 97.8% inhibition Caldanaerobius polysaccharolyticus
3.2.1.22 ZnCl2 1.0 mM, 99.3% inhibition Caldanaerobius polysaccharolyticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.22 0.29 0.35 p-nitrophenyl alpha-D-galactopyranoside
-
 Caldanaerobius polysaccharolyticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.22 80000
-
 2 * 80000, SDS-PAGE Caldanaerobius polysaccharolyticus
3.2.1.22 176000
-
 gel filtration Caldanaerobius polysaccharolyticus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.22 Caldanaerobius polysaccharolyticus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.22
-
 Caldanaerobius polysaccharolyticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.22 247.1
-
-
 Caldanaerobius polysaccharolyticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.22 melibiose + H2O
-
 Caldanaerobius polysaccharolyticus alpha-D-galactose + alpha-D-glucose
-
 ?
3.2.1.22 additional information activity with soy molasses Caldanaerobius polysaccharolyticus ?
-
 ?
3.2.1.22 p-nitrophenyl alpha-D-galactopyranoside + H2O
-
 Caldanaerobius polysaccharolyticus p-nitrophenol + alpha-D-galactose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.22 dimer 2 * 80000, SDS-PAGE Caldanaerobius polysaccharolyticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.22 78
-
-
 Caldanaerobius polysaccharolyticus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.22 65
-
 36.5 h, 12% loss of activity Caldanaerobius polysaccharolyticus
3.2.1.22 70
-
 36.5 h, 16% loss of activity Caldanaerobius polysaccharolyticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.22 7.5 8
-
 Caldanaerobius polysaccharolyticus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.22 5.5 10 pH 5.5: about 60% of maximal activity, pH 10.0: about 45% of maximal activity Caldanaerobius polysaccharolyticus