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Literature summary extracted from

  • Wang, S.L.; Hsiao, W.J.; Chang, W.T.
    Purification and characterization of an antimicrobial chitinase extracellularly produced by Monascus purpureus CCRC31499 in a shrimp and crab shell powder medium (2002), J. Agric. Food Chem., 50, 2249-2255.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.14 acetone
-
 Monascus purpureus
3.2.1.14 Hg2+
-
 Monascus purpureus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.14 extracellular
-
 Monascus purpureus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.14 Fe2+
-
 Monascus purpureus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.14 81000
-
 x * 81000, SDS-PAGE Monascus purpureus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Monascus purpureus
-
 CCRC31499
-
3.2.1.14 Monascus purpureus CCRC 31499
-
 CCRC31499
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14
-
 Monascus purpureus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.14 0.85
-
 with ethylene glycol chitin as substrate Monascus purpureus
3.2.1.14 1.02
-
 after Sephacryl S-200 purification step with colloidal chitin as substrate Monascus purpureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 colloidal chitin + H2O
-
 Monascus purpureus ?
-
 ?
3.2.1.14 colloidal chitin + H2O
-
 Monascus purpureus CCRC 31499 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 ? x * 81000, SDS-PAGE Monascus purpureus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 40
-
-
 Monascus purpureus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.14 100
-
 after 2 min loss of more than 40% enzyme activity Monascus purpureus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 7
-
-
 Monascus purpureus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.14 6 8
-
 Monascus purpureus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.14 Monascus purpureus chromatofocusing
-
 4.9