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Literature summary extracted from

  • Lakaye, B.; Makarchikov, A.F.; Wins, P.; Margineanu, I.; Roland, S.; Lins, L.; Aichour, R.; Lebeau, L.; El Moualij, B.; Zorzi, W.; Coumans, B.; Grisar, T.; Bettendorff, L.
    Human recombinant thiamine triphosphatase: purification, secondary structure and catalytic properties (2004), Int. J. Biochem. Cell Biol., 36, 1348-1364.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.28 expression in Escherichia coli, the recombinant enzyme is completely functional Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.28 ([2-[3-(4-Amino-2-methyl-pyrimidin-5-ylmethyl)-4-methyl-thiazol-5-yl]-ethoxy]-phosphonomethyl-phosphinoylmethyl)-phosphonic acid
-
 Homo sapiens
3.6.1.28 ATP very poor inhibitor Homo sapiens
3.6.1.28 Ca2+ inhibits by competition with Mg2+ Homo sapiens
3.6.1.28 SDS IC50: about 0.3% W/v. The inhibition is partially reversible, probably due to correct refolding of the denatured enzyme Homo sapiens
3.6.1.28 thiamine triphosphate
-
 Homo sapiens
3.6.1.28 Zn2+ inhibits at micromolar concentrations at pH 8.0, IC50: about 0.015-0.02 mM. Activates at pH 6.0 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.28 0.154
-
 thiamine triphosphate untagged recombinant enzyme Homo sapiens
3.6.1.28 3.7
-
 ATP in presence of 8 mM ATP Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.6.1.28 soluble
-
 Homo sapiens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.28 Mg2+ required Homo sapiens
3.6.1.28 Zn2+ inhibits at micromolar concentrations at pH 8.0, activates at pH 6.0 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.28 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.28
-
 Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.28 140
-
 purified recombinant enzyme Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.28 ATP + H2O the catalytic efficiency is about 4 orders of magnitude lower than for thiamine triphosphate Homo sapiens ADP + phosphate
-
 ?
3.6.1.28 thiamine triphosphate + H2O
-
 Homo sapiens thiamine diphosphate + phosphate
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.6.1.28 55 60 30 min, stable Homo sapiens
3.6.1.28 68
-
 30 min, 50% loss of activity Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.28 0.24
-
 ATP in presence of 8 mM Mg2+ Homo sapiens
3.6.1.28 140
-
 thiamine triphosphate
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.28 8.5
-
-
 Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.6.1.28 7 9.5 pH 7.0: about 60% of maximal activity, pH 9.5: about 75% of maximal activity Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.6.1.28 0.95
-
 thiamine triphosphate
-
 Homo sapiens
3.6.1.28 1.2
-
 ([2-[3-(4-Amino-2-methyl-pyrimidin-5-ylmethyl)-4-methyl-thiazol-5-yl]-ethoxy]-phosphonomethyl-phosphinoylmethyl)-phosphonic acid
-
 Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.6.1.28 0.015 0.02 inhibits at micromolar concentrations at pH 8.0, IC50: about 0.015-0.02 mM. Activates at pH 6.0 Homo sapiens Zn2+