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Literature summary extracted from

  • Ye, X.Y.; Ng, T.B.; Cheng, K.J.
    Purification and characterization of a cellulase from the ruminal fungus Orpinomyces joyonii cloned in Escherichia coli (2001), Int. J. Biochem. Cell Biol., 33, 87-94.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.4 expression in Escherichia coli Orpinomyces joyonii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.4 additional information
-
 additional information the Km-values for barley beta-glucan and carboxymethylcellulose are 0.32 mg/ml and 0.5 mg/ml Orpinomyces joyonii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.4 56463
-
 x * 56463, calculation from nucleotide sequence Orpinomyces joyonii
3.2.1.4 58000
-
 x * 58000, SDS-PAGE Orpinomyces joyonii

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Orpinomyces joyonii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.4
-
 Orpinomyces joyonii

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 beta-glucan + H2O
-
 Orpinomyces joyonii ?
-
 ?
3.2.1.4 carboxymethyl cellulose + H2O 68% of the activity with barley beta-glucan Orpinomyces joyonii ?
-
 ?
3.2.1.4 cellobioside + H2O about 2% of the activity with barley beta-glucan Orpinomyces joyonii ?
-
 ?
3.2.1.4 cellotrioside + H2O 7.4% of the activity with barley beta-glucan Orpinomyces joyonii ?
-
 ?
3.2.1.4 lichenan + H2O 9% of the activity with barley beta-glucan Orpinomyces joyonii ?
-
 ?
3.2.1.4 additional information no activity with laminarin, avicel, pullulan and pachyman Orpinomyces joyonii ?
-
 ?
3.2.1.4 xylan + H2O about 2% of the activity with barley beta-glucan Orpinomyces joyonii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.4 ? x * 58000, SDS-PAGE Orpinomyces joyonii
3.2.1.4 ? x * 56463, calculation from nucleotide sequence Orpinomyces joyonii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 40
-
 reaction with carboxymethyl cellulose or barley beta-glucan Orpinomyces joyonii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.4 40
-
 10 min, stable Orpinomyces joyonii
3.2.1.4 50
-
 10 min, about 10% loss of activity Orpinomyces joyonii
3.2.1.4 55
-
 10 min, about 30% loss of activity with barley beta-glucan and about 60% loss of activity with carboxymethylcellulose Orpinomyces joyonii
3.2.1.4 60
-
 10 min, about 50% loss of activity with barley beta-glucan and about 75% loss of activity with carboxymethylcellulose Orpinomyces joyonii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 5.5
-
 reaction with carboxymethyl cellulose Orpinomyces joyonii
3.2.1.4 6
-
 reaction barley beta-glucan Orpinomyces joyonii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.4 5 8.5 pH 4.5: about 15% of maximal activity, pH 5.0: about 95% of maximal activity, pH 8.5: about 50% of maximal activity, reaction with carboxymethyl cellulose Orpinomyces joyonii
3.2.1.4 5 7.5 pH 5.0: about 75% of maximal activity, pH 7.5: about 50% of maximal activity, reaction with barley beta-glucan Orpinomyces joyonii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.4 5 7.5 4°C, overnight, stable Orpinomyces joyonii