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Literature summary extracted from

  • Kuznetsova, E.; Proudfoot, M.; Sanders, S.A.; Reinking, J.; Savchenko, A.; Arrowsmith, C.H.; Edwards, A.M.; Yakunin, A.F.
    Enzyme genomics: Application of general enzymatic screens to discover new enzymes (2005), FEMS Microbiol. Rev., 29, 263-279.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.3
-
 Escherichia coli
3.1.1.1
-
 Escherichia coli
3.1.2.2
-
 Escherichia coli
3.1.3.5
-
 Pseudomonas aeruginosa
3.1.3.41
-
 Thermoplasma acidophilum
3.1.3.58
-
 Thermotoga maritima
3.1.4.1
-
 Escherichia coli

General Stability

EC Number General Stability Organism
1.2.1.3 stable Escherichia coli
3.1.1.1 stable Escherichia coli
3.1.2.2 stable Escherichia coli
3.1.3.5 stable Pseudomonas aeruginosa
3.1.3.41 stable Thermoplasma acidophilum
3.1.3.58 stable Thermotoga maritima
3.1.4.1 stable Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.3 0.69
-
 Succinic semialdehyde +/- 0.22 Escherichia coli
1.2.1.3 91
-
 decanal +/- 20 Escherichia coli
3.1.3.5 0.39
-
 5'-UMP +/- 0.02 Pseudomonas aeruginosa
3.1.3.58 0.1526
-
 erythrose 4-phosphate +/- 0.055 Thermotoga maritima
3.1.3.58 0.2
-
 fructose 6-phosphate +/- 0.02 Thermotoga maritima

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.5 Mn2+ activates Pseudomonas aeruginosa
3.1.3.58 Co2+ activates Thermotoga maritima
3.1.3.58 Mg2+ activates Thermotoga maritima
3.1.3.58 Mn2+ activates Thermotoga maritima
3.1.3.58 Ni2+ activates Thermotoga maritima
3.1.4.1 Mn2+ activates Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.3 Escherichia coli P76217
-
-
3.1.1.1 Escherichia coli P04335
-
-
3.1.2.2 Escherichia coli
-
-
-
3.1.2.2 Escherichia coli P0A8Y8
-
-
3.1.2.2 Escherichia coli P0A8Z0
-
-
3.1.2.2 Escherichia coli P0A8Z3
-
-
3.1.2.2 Escherichia coli P33018
-
-
3.1.2.2 Escherichia coli P37355
-
-
3.1.2.2 Escherichia coli P39298
-
-
3.1.2.2 Escherichia coli P46130
-
-
3.1.2.2 Escherichia coli P75736
-
-
3.1.2.2 Escherichia coli P76561
-
-
3.1.2.2 Escherichia coli P77781
-
-
3.1.3.5 Pseudomonas aeruginosa Q9I767
-
-
3.1.3.41 Thermoplasma acidophilum Q9HJW8
-
-
3.1.3.58 Thermotoga maritima Q9X0Y1
-
-
3.1.4.1 Escherichia coli P37049
-
-
3.1.4.1 Escherichia coli P39300
-
-
3.1.4.1 Escherichia coli P67095
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.3 0.69
-
 +/- 0.02, succinic semialdehyde Escherichia coli
1.2.1.3 5
-
 +/- 0.5, decanal Escherichia coli
3.1.3.5 3.14
-
 +/- 0.05, 5'-UMP Pseudomonas aeruginosa
3.1.3.58 2.63
-
 +/- 0.56, erythrose 4-phosphate Thermotoga maritima

Storage Stability

EC Number Storage Stability Organism
1.2.1.3 4°C, 5% glycerol, 0.5 M NaCl, pH 7.5, no loss in activity after several months Escherichia coli
3.1.1.1 4°C, 5% glycerol, 0.5 M NaCl, pH 7.5, no loss in activity after several months Escherichia coli
3.1.2.2 4°C, 5% glycerol, 0.5 M NaCl, pH 7.5, no loss in activity after several months Escherichia coli
3.1.3.5 4°C, 5% glycerol, 0.5 M NaCl, pH 7.5, no loss in activity after several months Pseudomonas aeruginosa
3.1.3.41 4°C, 5% glycerol, 0.5 M NaCl, pH 7.5, no loss in activity after several months Thermoplasma acidophilum
3.1.3.58 4°C, 5% glycerol, 0.5 M NaCl, pH 7.5, no loss in activity after several months Thermotoga maritima
3.1.4.1 4oC, 5% glycerol, 0.5 M NaCl, pH 7.5, no loss in activity after several months Escherichia coli
3.1.4.1 4°C, 5% glycerol, 0.5 M NaCl, pH 7.5, no loss in activity after several months Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.3 decanal + NAD+ + H2O
-
 Escherichia coli decanaote + NADH
-
 ?
1.2.1.3 succinic semialdehyde + NAD+ + H2O
-
 Escherichia coli succinate + NADH
-
 ?
3.1.1.1 p-nitrophenyl butyrate + H2O
-
 Escherichia coli p-nitrophenol + butanoate
-
 ?
3.1.2.2 malonyl-CoA + H2O
-
 Escherichia coli malonate + CoA
-
 ?
3.1.2.2 p-nitrophenyl butyrate + H2O
-
 Escherichia coli p-nitrophenol + butanoate
-
 ?
3.1.2.2 palmitoyl-CoA + H2O
-
 Escherichia coli palmitate + CoA
-
 ?
3.1.3.5 5'-IMP + H2O
-
 Pseudomonas aeruginosa inosine + phosphate
-
 ?
3.1.3.5 5'-UMP + H2O
-
 Pseudomonas aeruginosa uridine + phosphate
-
 ?
3.1.3.5 p-nitrophenyl phosphate + H2O
-
 Pseudomonas aeruginosa p-nitrophenol + phosphate
-
 ?
3.1.3.41 p-nitrophenyl phosphate + H2O
-
 Thermoplasma acidophilum p-nitrophenol + phosphate
-
 ?
3.1.3.58 2-Deoxy-D-glucose 6-phosphate + H2O
-
 Thermotoga maritima 2-Deoxy-D-glucose + phosphate
-
 ?
3.1.3.58 D-fructose 6-phosphate + H2O
-
 Thermotoga maritima D-fructose + phosphate
-
 ?
3.1.3.58 D-mannose 6-phosphate + H2O
-
 Thermotoga maritima D-mannose + phosphate
-
 ?
3.1.3.58 erythrose 4-phosphate + H2O
-
 Thermotoga maritima erythrose + phosphate
-
 ?
3.1.3.58 p-nitrophenyl phosphate + H2O
-
 Thermotoga maritima p-nitrophenol + phosphate
-
 ?
3.1.4.1 bis-p-nitrophenyl phosphate + H2O
-
 Escherichia coli p-nitrophenol + p-nitrophenyl phosphate
-
 ?
3.1.4.1 p-nitrophenyl 5'-thymidine monophosphate + H2O
-
 Escherichia coli p-nitrophenol + 5'-thymidine monophosphate
-
 ?
3.1.4.1 p-nitrophenylphosphorylcholine + H2O
-
 Escherichia coli p-nitrophenol + phosphorylcholine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.1 esterase
-
 Escherichia coli
3.1.2.2 esterase
-
 Escherichia coli
3.1.2.2 thioesterase
-
 Escherichia coli
3.1.4.1 phosphodiesterase
-
 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.3 8.5
-
-
 Escherichia coli
3.1.3.5 7.4
-
-
 Pseudomonas aeruginosa

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.3 NAD+
-
 Escherichia coli