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Literature summary extracted from

  • Lim, S.; Schroder, I.; Monbouquette, H.G.
    A thermostable shikimate 5-dehydrogenase from the archaeon Archaeoglobus fulgidus (2004), FEMS Microbiol. Lett., 238, 101-106.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.25
-
 Archaeoglobus fulgidus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.25 0.17
-
 shikimate 87°C, pH 7.3 Archaeoglobus fulgidus
1.1.1.25 0.19
-
 NADP+ 87°C, pH 7.3 Archaeoglobus fulgidus
1.1.1.25 11.4
-
 NAD+ 87°C, pH 7.3 Archaeoglobus fulgidus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.25 29140
-
 2 * 32000, SDS-PAGE, 2 * 29140, deduced from gene sequence Archaeoglobus fulgidus
1.1.1.25 32000
-
 2 * 32000, SDS-PAGE, 2 * 29140, deduced from gene sequence Archaeoglobus fulgidus
1.1.1.25 58000
-
 gel filtration Archaeoglobus fulgidus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.25 Archaeoglobus fulgidus
-
 recombinant enzyme expressed in Escherichia coli
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.25 732
-
 87°C, pH 7.3, cosubstrate NADP+ Archaeoglobus fulgidus
1.1.1.25 1479
-
 87°C, pH 7.3, cosubstrate NAD+ Archaeoglobus fulgidus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.25 3-dehydroshikimate + NADPH NADH may substitute for NADPH Archaeoglobus fulgidus shikimate + NADP+
-
 r
1.1.1.25 shikimate + NAD+
-
 Archaeoglobus fulgidus 3-dehydroshikimate + NADH + H+
-
 r
1.1.1.25 shikimate + NADP+
-
 Archaeoglobus fulgidus 3-dehydroshikimate + NADPH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.25 dimer 2 * 32000, SDS-PAGE, 2 * 29140, deduced from gene sequence Archaeoglobus fulgidus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.25 87
-
 assay at Archaeoglobus fulgidus
1.1.1.25 95
-
 and above Archaeoglobus fulgidus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.25 60
-
 half-life about 15 days, no addition of salt Archaeoglobus fulgidus
1.1.1.25 60
-
 half-life about 47 days, presence of 1 M NaCl Archaeoglobus fulgidus
1.1.1.25 87
-
 half-life about 2 h, no addition of salt Archaeoglobus fulgidus
1.1.1.25 87
-
 half-life about 60 h, presence of 1 M NaCl Archaeoglobus fulgidus
1.1.1.25 100
-
 half-life about 2.1 h, presence of 1 M NaCl Archaeoglobus fulgidus
1.1.1.25 100
-
 half-life about 30 min, no addition of salt Archaeoglobus fulgidus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.25 7 7.5
-
 Archaeoglobus fulgidus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.25 NADP+
-
 Archaeoglobus fulgidus
1.1.1.25 NADPH
-
 Archaeoglobus fulgidus