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Literature summary extracted from

  • Korakli, M.; Vogel, R.F.
    Purification and characterisation of mannitol dehydrogenase from Lactobacillus sanfranciscensis (2003), FEMS Microbiol. Lett., 220, 281-286.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.67 EDTA 10 mM, activates to 158% of control Fructilactobacillus sanfranciscensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.67 FeCl2 10 mM, 88% inhibition Fructilactobacillus sanfranciscensis
1.1.1.67 MnCl2 10 mM, 48% inhibition Fructilactobacillus sanfranciscensis
1.1.1.67 ZnCl2 10 mM, 60% inhibition Fructilactobacillus sanfranciscensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.67 24
-
 D-fructose 25°C, pH 7.3 Fructilactobacillus sanfranciscensis
1.1.1.67 78
-
 D-mannitol 25°C Fructilactobacillus sanfranciscensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.67 CaCl2 10 mM, activates to 173% of control Fructilactobacillus sanfranciscensis
1.1.1.67 KCl 10 mM, activates to 127% of control Fructilactobacillus sanfranciscensis
1.1.1.67 LiCl 10 mM, activates to 126% of control Fructilactobacillus sanfranciscensis
1.1.1.67 MgCl2 10 mM, activates to 149% of control Fructilactobacillus sanfranciscensis
1.1.1.67 NH4Cl 10 mM, activates to 137% of control Fructilactobacillus sanfranciscensis
1.1.1.67 NiCl2 10 mM, activates to 106% of control Fructilactobacillus sanfranciscensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.67 44000
-
 1 * 44000, SDS-PAGE Fructilactobacillus sanfranciscensis
1.1.1.67 53000
-
 gel filtration Fructilactobacillus sanfranciscensis

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.67 Fructilactobacillus sanfranciscensis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.67
-
 Fructilactobacillus sanfranciscensis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.67 15.48
-
-
 Fructilactobacillus sanfranciscensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.67 D-mannitol + NAD+
-
 Fructilactobacillus sanfranciscensis D-fructose + NADH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.67 monomer 1 * 44000, SDS-PAGE Fructilactobacillus sanfranciscensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.67 35
-
 reduction of D-fructose Fructilactobacillus sanfranciscensis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.67 20 40 20°C: about 85% of maximal activity, 40°C: about 70% of maximal activity, reduction of D-fructose Fructilactobacillus sanfranciscensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.67 5.8
-
 reduction of D-fructose Fructilactobacillus sanfranciscensis
1.1.1.67 8
-
 oxidation of D-mannitol Fructilactobacillus sanfranciscensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.67 4.5 7 pH 4.5: about 80% of maximal activity, pH 7.0: about 60% of maximal activity, reduction of D-fructose Fructilactobacillus sanfranciscensis
1.1.1.67 6.8 8.3 pH 6.8: about 20% of maximal activity, pH 8.3: about 55% of maximal activity, oxidation of D-mannitol Fructilactobacillus sanfranciscensis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.67 NAD+
-
 Fructilactobacillus sanfranciscensis
1.1.1.67 NADH
-
 Fructilactobacillus sanfranciscensis