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Literature summary extracted from

  • Klein, M.; Kaltwasser, H.; Jahns, T.
    Isolation of a novel, phosphate-activated glutaminase from Bacillus pasteurii (2002), FEMS Microbiol. Lett., 206, 63-67.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.2 additional information activity is not significantly impaired with 2-oxoglutarate, pyruvate, succinate and citrate, no inhibition with L-glutamate, NH4+ and L-aspartate Sporosarcina pasteurii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.2 additional information
-
 additional information Michaelis-Menten kinetics Sporosarcina pasteurii
3.5.1.2 9.5
-
 L-glutamine pH 9.0, at 37ºC, H2O as co-substrate Sporosarcina pasteurii
3.5.1.2 15
-
 gamma-glutamyl hydroxamate pH 9.0, at 37ºC, NH2OH as co-substrate Sporosarcina pasteurii
3.5.1.2 17
-
 L-glutamine pH 9.0, at 37ºC, NH2OH as co-substrate Sporosarcina pasteurii
3.5.1.2 24
-
 L-Asn pH 9.0, at 37ºC, H2O as co-substrate Sporosarcina pasteurii
3.5.1.2 83
-
 D-glutamine pH 9.0, at 37ºC, H2O as co-substrate Sporosarcina pasteurii
3.5.1.2 500
-
 L-Glutamic acid pH 9.0, at 37ºC, NH2OH as co-substrate Sporosarcina pasteurii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.2 cytoplasm
-
 Sporosarcina pasteurii 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.2 arsenate 50 mM, 800% of the relative activity without addition Sporosarcina pasteurii
3.5.1.2 additional information no effect with NaCl, no significant alteration with KCl, MgCl2, MnCl2, CaCl2 and NH4Cl Sporosarcina pasteurii
3.5.1.2 phosphate 50 mM, 900% of the relative activity without addition Sporosarcina pasteurii
3.5.1.2 sulfate 50 mM, 700% of the relative activity without addition Sporosarcina pasteurii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.2 55000
-
 2 * 55000, SDS-PAGE Sporosarcina pasteurii
3.5.1.2 110000
-
 PAGE under nondenaturing conditions Sporosarcina pasteurii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.2 L-glutamine + H2O Sporosarcina pasteurii glutamine catabolism L-glutamate + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.2 Sporosarcina pasteurii
-
 ATCC 11859
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.5.1.2 no modification
-
 Sporosarcina pasteurii

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.2 by two-step ammonium sulfate precipitation, hydrophobic interaction chromatography on phenyl-Sepharose, anion exchange chromatography on DEAE-cellulose, hydrophobic interaction chromatography on phenyl-Superose and anion exchange chromatography using Mono Q, 762fold Sporosarcina pasteurii

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.2 culture medium
-
 Sporosarcina pasteurii
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.2 234
-
-
 Sporosarcina pasteurii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.2 D-Asn + H2O less 0.1% of the activity with L-glutamine and H2O as co-substrate Sporosarcina pasteurii D-aspartate + NH3
-
 ?
3.5.1.2 D-glutamine + H2O 8.1% of the activity with L-glutamine and H2O as co-substrate Sporosarcina pasteurii D-glutamate + NH3
-
 ?
3.5.1.2 gamma-glutamyl hydroxamate + NH2OH 36.3% of the activity with L-glutamine and H2O as co-substrate Sporosarcina pasteurii ?
-
 ?
3.5.1.2 L-Asn + H2O 20.6% of the activity with L-glutamine and H2O as co-substrate Sporosarcina pasteurii L-aspartate + NH3
-
 ?
3.5.1.2 L-glutamic acid + NH2OH 0.9% of the activity with L-glutamine and H2O as co-substrate Sporosarcina pasteurii gamma-glutamyl hydroxamate
-
 ?
3.5.1.2 L-glutamine + H2O
-
 Sporosarcina pasteurii L-glutamate + NH3
-
 ?
3.5.1.2 L-glutamine + H2O glutamine catabolism Sporosarcina pasteurii L-glutamate + NH3
-
 ?
3.5.1.2 L-glutamine + NH2OH 10.3% of the activity with L-glutamine and H2O as co-substrate Sporosarcina pasteurii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.2 homodimer 2 * 55000, SDS-PAGE Sporosarcina pasteurii

Synonyms

EC Number Synonyms Comment Organism
3.5.1.2 L-glutamine amidohydrolase
-
 Sporosarcina pasteurii
3.5.1.2 phosphate activated glutaminase
-
 Sporosarcina pasteurii
3.5.1.2 phosphate-activated L-glutamine amidohydrolase
-
 Sporosarcina pasteurii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.2 37
-
-
 Sporosarcina pasteurii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.2 9
-
-
 Sporosarcina pasteurii

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.5.1.2 Sporosarcina pasteurii isoelectric focusing
-
 4.9