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Literature summary extracted from
- Bioconversion of pyrimidine by resting cells of quinoline-degrading bacteria (1999), FEMS Microbiol. Lett., 176, 291-299.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 1.3.99.17 | synthesis | production of uracil in supernatant of resting cells with a yield of more than 98% | Comamonas testosteroni |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.3.99.17 | additional information | no inbibition by pyrimidine or uracil | Comamonas testosteroni |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.3.99.17 | Comamonas testosteroni | - |
growth on quinoline | - |
| 1.3.99.17 | Comamonas testosteroni 63 | - |
growth on quinoline | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.3.99.17 | 0.0057 | - |
substrate substrate pyrimidine-4-one, crude extract, pH 7.5, 25°C | Comamonas testosteroni |
| 1.3.99.17 | 0.064 | - |
substrate pyrimidine, crude extract, pH 7.5, 25°C | Comamonas testosteroni |
| 1.3.99.17 | 0.237 | - |
substrate quinoline, crude extract, pH 7.5, 25°C | Comamonas testosteroni |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.3.99.17 | additional information | no substrate: uracil | Comamonas testosteroni | ? | - |
? |  |
| 1.3.99.17 | additional information | no substrate: uracil | Comamonas testosteroni 63 | ? | - |
? | |
| 1.3.99.17 | pyrimidine + p-iodonitro-tetrazolium violet + H2O | - |
Comamonas testosteroni | pyrimidine-4-one + reduced p-iodomitro-tetrazolium violet | - |
? | |
| 1.3.99.17 | pyrimidine + p-iodonitro-tetrazolium violet + H2O | - |
Comamonas testosteroni 63 | pyrimidine-4-one + reduced p-iodomitro-tetrazolium violet | - |
? | |
| 1.3.99.17 | pyrimidine-4-one + p-iodonitro-tetrazolium violet + H2O | - |
Comamonas testosteroni | ? + reduced p-iodonitro-tetrazolium violet | - |
? | |
| 1.3.99.17 | pyrimidine-4-one + p-iodonitro-tetrazolium violet + H2O | - |
Comamonas testosteroni 63 | ? + reduced p-iodonitro-tetrazolium violet | - |
? | |
| 1.3.99.17 | quinoline + p-iodonitro-tetrazolium violet + H2O | - |
Comamonas testosteroni | isoquinoline-1-one + reduced p-iodonitro-tetrazolium violet | - |
? | |
| 1.3.99.17 | quinoline + p-iodonitro-tetrazolium violet + H2O | - |
Comamonas testosteroni 63 | isoquinoline-1-one + reduced p-iodonitro-tetrazolium violet | - |
? | |
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