BRENDA - Enzyme Database

Bioconversion of pyrimidine by resting cells of quinoline-degrading bacteria

Fetzner, S.; FEMS Microbiol. Lett. 176, 291-299 (1999)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
1.3.99.17
synthesis
production of uracil in supernatant of resting cells with a yield of more than 98%
Comamonas testosteroni
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.3.99.17
additional information
no inbibition by pyrimidine or uracil
Comamonas testosteroni
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.3.99.17
Comamonas testosteroni
-
growth on quinoline
-
1.3.99.17
Comamonas testosteroni 63
-
growth on quinoline
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.3.99.17
0.0057
-
substrate substrate pyrimidine-4-one, crude extract, pH 7.5, 25°C
Comamonas testosteroni
1.3.99.17
0.064
-
substrate pyrimidine, crude extract, pH 7.5, 25°C
Comamonas testosteroni
1.3.99.17
0.237
-
substrate quinoline, crude extract, pH 7.5, 25°C
Comamonas testosteroni
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.3.99.17
additional information
no substrate: uracil
655595
Comamonas testosteroni
?
-
-
-
?
1.3.99.17
additional information
no substrate: uracil
655595
Comamonas testosteroni 63
?
-
-
-
?
1.3.99.17
pyrimidine + p-iodonitro-tetrazolium violet + H2O
-
655595
Comamonas testosteroni
pyrimidine-4-one + reduced p-iodomitro-tetrazolium violet
-
-
-
?
1.3.99.17
pyrimidine + p-iodonitro-tetrazolium violet + H2O
-
655595
Comamonas testosteroni 63
pyrimidine-4-one + reduced p-iodomitro-tetrazolium violet
-
-
-
?
1.3.99.17
pyrimidine-4-one + p-iodonitro-tetrazolium violet + H2O
-
655595
Comamonas testosteroni
? + reduced p-iodonitro-tetrazolium violet
-
-
-
?
1.3.99.17
pyrimidine-4-one + p-iodonitro-tetrazolium violet + H2O
-
655595
Comamonas testosteroni 63
? + reduced p-iodonitro-tetrazolium violet
-
-
-
?
1.3.99.17
quinoline + p-iodonitro-tetrazolium violet + H2O
-
655595
Comamonas testosteroni
isoquinoline-1-one + reduced p-iodonitro-tetrazolium violet
-
-
-
?
1.3.99.17
quinoline + p-iodonitro-tetrazolium violet + H2O
-
655595
Comamonas testosteroni 63
isoquinoline-1-one + reduced p-iodonitro-tetrazolium violet
-
-
-
?
Application (protein specific)
EC Number
Application
Commentary
Organism
1.3.99.17
synthesis
production of uracil in supernatant of resting cells with a yield of more than 98%
Comamonas testosteroni
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.3.99.17
additional information
no inbibition by pyrimidine or uracil
Comamonas testosteroni
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.3.99.17
0.0057
-
substrate substrate pyrimidine-4-one, crude extract, pH 7.5, 25°C
Comamonas testosteroni
1.3.99.17
0.064
-
substrate pyrimidine, crude extract, pH 7.5, 25°C
Comamonas testosteroni
1.3.99.17
0.237
-
substrate quinoline, crude extract, pH 7.5, 25°C
Comamonas testosteroni
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.3.99.17
additional information
no substrate: uracil
655595
Comamonas testosteroni
?
-
-
-
?
1.3.99.17
additional information
no substrate: uracil
655595
Comamonas testosteroni 63
?
-
-
-
?
1.3.99.17
pyrimidine + p-iodonitro-tetrazolium violet + H2O
-
655595
Comamonas testosteroni
pyrimidine-4-one + reduced p-iodomitro-tetrazolium violet
-
-
-
?
1.3.99.17
pyrimidine + p-iodonitro-tetrazolium violet + H2O
-
655595
Comamonas testosteroni 63
pyrimidine-4-one + reduced p-iodomitro-tetrazolium violet
-
-
-
?
1.3.99.17
pyrimidine-4-one + p-iodonitro-tetrazolium violet + H2O
-
655595
Comamonas testosteroni
? + reduced p-iodonitro-tetrazolium violet
-
-
-
?
1.3.99.17
pyrimidine-4-one + p-iodonitro-tetrazolium violet + H2O
-
655595
Comamonas testosteroni 63
? + reduced p-iodonitro-tetrazolium violet
-
-
-
?
1.3.99.17
quinoline + p-iodonitro-tetrazolium violet + H2O
-
655595
Comamonas testosteroni
isoquinoline-1-one + reduced p-iodonitro-tetrazolium violet
-
-
-
?
1.3.99.17
quinoline + p-iodonitro-tetrazolium violet + H2O
-
655595
Comamonas testosteroni 63
isoquinoline-1-one + reduced p-iodonitro-tetrazolium violet
-
-
-
?