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Literature summary extracted from
- One functional subunit is sufficient for catalytic activity and substrate specificity of Escherichia coli endoribonuclease III artificial heterodimers (2002), FEBS Lett., 518, 93-96.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.26.3 | expression of a His-tagged E117Q mutant homodimer, a GST-tagged wild-type homodimer, and a His-tagged and GST-tagged wild-type/mutant heterodimer in strain BL21 | Escherichia coli |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.1.26.3 | E117Q | mutant enzyme can still bind to the substrate RNA in presence of Mg2+ but cannot cleave it | Escherichia coli |
| 3.1.26.3 | additional information | construction of a heterodimer comprising one functional wild-type subunit and one inactive E117Q mutant subunit, which carries the E117Q mutation allowing the mutant subunit to bind but not cleave the substrate, the functional subunit is sufficient for catalytic activity of the heterodimer | Escherichia coli |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.26.3 | KCl | enzyme is sensitive to high salt conditions, above 50 mM, the mutant homodimer and the heterodimer are more sensitive than the wild-type | Escherichia coli |  |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.26.3 | Mg2+ | - |
Escherichia coli | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.26.3 | 25600 | - |
2 * 25600, wild-type enzyme, SDS-PAGE | Escherichia coli |
| 3.1.26.3 | 54000 | - |
recombinant His-tagged mutant homodimer, gel filtration | Escherichia coli |
| 3.1.26.3 | 78000 | - |
recombinant His-tagged and GST-tagged wild-type/mutant heterodimer, gel filtration | Escherichia coli |
| 3.1.26.3 | 103000 | - |
recombinant GST-tagged wild-type homodimer, gel filtration | Escherichia coli |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.26.3 | Escherichia coli | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.26.3 | recombinant tagged heterodimer and homodimers from strain BL21 to homogeneity | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.26.3 | dsRNA + H2O | one functional monomer is sufficient for cleavage activity of the dimer | Escherichia coli | mature RNA | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.26.3 | dimer | 2 * 25600, wild-type enzyme, SDS-PAGE | Escherichia coli |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.26.3 | endoribonuclease III | - |
Escherichia coli |
| 3.1.26.3 | RNase III | - |
Escherichia coli |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.26.3 | 37 | - |
assay at | Escherichia coli |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.26.3 | 7.5 | - |
assay at | Escherichia coli |
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Release 2023.1 (January 2023)
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