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Literature summary extracted from

  • Hurtado-Guerrrero, R.; Pena-Diaz, J.; Montalvetti, A.; Ruiz-Perez, L.M.; Gonzalez-Pacanowska, D.
    Kinetic properties and inhibition of Trypanosoma cruzi 3-hydroxy-3-methylglutaryl CoA reductase (2002), FEBS Lett., 510, 141-144.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.34 expression in Escherichia coli strain BL21(DE3) as soluble protein Trypanosoma cruzi

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.34 cerivastatin biphasic inhibition mechanism, slow, tight-binding type of inhibitor Trypanosoma cruzi
1.1.1.34 fluvastatin competitive Trypanosoma cruzi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.34 additional information
-
 additional information kinetic analysis Trypanosoma cruzi
1.1.1.34 0.013
-
 (S)-3-hydroxy-3-methylglutaryl-CoA pH 6.8, 25°C Trypanosoma cruzi
1.1.1.34 0.043
-
 NADPH pH 6.8, 25°C Trypanosoma cruzi
1.1.1.34 0.074
-
 CoA pH 8.3, 25°C Trypanosoma cruzi
1.1.1.34 0.22
-
 NADP+ pH 8.3, 25°C Trypanosoma cruzi
1.1.1.34 0.252
-
 (R)-mevalonate pH 8.3, 25°C Trypanosoma cruzi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.34 (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+ Trypanosoma cruzi
-
 (R)-mevalonate + CoA + 2 NADP+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.34 Trypanosoma cruzi
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.34 3.8fold, recombinant enzyme from Escherichia coli Trypanosoma cruzi

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.34 28
-
 purified recombinant enzyme Trypanosoma cruzi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.34 (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+
-
 Trypanosoma cruzi (R)-mevalonate + CoA + 2 NADP+
-
 r
1.1.1.34 (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+ the reduction of 3-hydroxy-3-methylglutaryl-CoA is preferred over oxidation of mevalonate Trypanosoma cruzi (R)-mevalonate + CoA + 2 NADP+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.34 tetramer
-
 Trypanosoma cruzi

Synonyms

EC Number Synonyms Comment Organism
1.1.1.34 3-hydroxy-3-methylglutaryl CoA reductase
-
 Trypanosoma cruzi
1.1.1.34 HMGR
-
 Trypanosoma cruzi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.34 25
-
 assay at Trypanosoma cruzi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.34 5.7 6.3 reduction of (S)-3-hydroxy-3-methylglutaryl-CoA Trypanosoma cruzi
1.1.1.34 8.7 9.6 oxidation of (R)-mevalonate Trypanosoma cruzi

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.34 NADPH
-
 Trypanosoma cruzi

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.34 0.027
-
 fluvastatin pH 6.8, 25°C, versus (S)-3-hydroxy-3-methylglutaryl-CoA Trypanosoma cruzi