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Literature summary extracted from

  • Conrad, C.; Evguenieva-Hackenberg, E.; Klug, G.
    Both N-terminal catalytic and C-terminal RNA binding domain contribute to substrate specificity and cleavage site selection of RNase III (2001), FEBS Lett., 509, 53-58.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.26.3 overexpression of mutant hybrid proteins in Escherichia coli strain HMS174 Escherichia coli
3.1.26.3 overexpression of mutant hybrid proteins in Escherichia coli strain HMS174 Rhodobacter capsulatus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.26.3 additional information construction of hybrid proteins consisiting of the N-terminal nuclease domain of Rhodobacter capsulatus and the C-terminal dsRNA-binding domain of Escherichia coli and vice versa, extension of the spacer region between the N-terminal and C-terminal domains does not alter the cleavage specificity Escherichia coli
3.1.26.3 additional information construction of hybrid proteins consisting of the N-terminal nuclease domain of Rhodobacter capsulatus and the C-terminal dsRNA-binding domain of Escherichia coli and vice versa Rhodobacter capsulatus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.26.3 KCl
-
 Escherichia coli
3.1.26.3 KCl
-
 Rhodobacter capsulatus
3.1.26.3 Mg2+ activity with recombinant hybrid enzyme mutants, overview Escherichia coli
3.1.26.3 Mg2+ activity with recombinant hybrid enzyme mutants, overview Rhodobacter capsulatus
3.1.26.3 Mn2+ activity with recombinant hybrid enzyme mutants, overview Escherichia coli
3.1.26.3 Mn2+ activity with recombinant hybrid enzyme mutants, overview Rhodobacter capsulatus
3.1.26.3 additional information metal ion specificity of the enzyme is determined by the N-terminus Escherichia coli
3.1.26.3 additional information metal ion specificity of the enzyme is determined by the N-terminus Rhodobacter capsulatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.26.3 ds-rRNA + H2O Escherichia coli
-
 mature ds-rRNA
-
 ?
3.1.26.3 ds-rRNA + H2O Rhodobacter capsulatus
-
 mature ds-rRNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.26.3 Escherichia coli
-
 gene rnc, strain JM109, recombinant hybrid enzymes consisiting of the N-terminal nuclease domain of Rhodobacter capsulatus and the C-terminal dsRNA-binding domain of Escherichia coli, and vice versa
-
3.1.26.3 Rhodobacter capsulatus
-
 gene rnc, strain 37b4, recombinant hybrid enzymes consisiting of the N-terminal nuclease domain of Rhodobacter capsulatus and the C-terminal dsRNA-binding domain of Escherichia coli, and vice versa
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.26.3 recombinant hybrid proteins to homogeneity Escherichia coli
3.1.26.3 recombinant hybrid proteins to homogeneity Rhodobacter capsulatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.26.3 ds-rRNA + H2O
-
 Escherichia coli mature ds-rRNA
-
 ?
3.1.26.3 ds-rRNA + H2O
-
 Rhodobacter capsulatus mature ds-rRNA
-
 ?
3.1.26.3 ds-rRNA + H2O double-strand RNA specific endonuclease Escherichia coli mature ds-rRNA
-
 ?
3.1.26.3 dsRNA + H2O double-strand RNA specific endonuclease Rhodobacter capsulatus mature RNA
-
 ?
3.1.26.3 additional information substrate specificity of the recombinant hybrid enzyme mutants, substrate specificity is determined by the catalytic N-terminus of the enzyme Escherichia coli ?
-
 ?
3.1.26.3 additional information substrate specificity of the recombinant hybrid enzyme mutants, substrate specificity is determined by the catalytic N-terminus of the enzyme Rhodobacter capsulatus ?
-
 ?
3.1.26.3 R1.1 RNA + H2O based on the R1.1 processing signal, which is encoded in the phage T7 genetic early region between genes 1.0 and 1.1, recombinant substrate from in vitro transcription, determination of cleaving positions for the recombinant hybrid enzyme mutants Escherichia coli fragments of R1.1 RNA
-
 ?
3.1.26.3 R1.1 RNA + H2O based on the R1.1 processing signal, which is encoded in the phage T7 genetic early region between genes 1.0 and 1.1, recombinant substrate from in vitro transcription, determination of cleaving positions for the recombinant hybrid enzyme mutants Rhodobacter capsulatus fragments of R1.1 RNA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.26.3 RNase III
-
 Escherichia coli
3.1.26.3 RNase III
-
 Rhodobacter capsulatus