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Literature summary extracted from

  • Irwin, J.A.; Lynch, S.V.; Coughlan, S.; Baker, P.J.; Gudmundsson, H.M.; Alfredsson, G.A.; Rice, D.W.; Engel, P.C.
    Alanine dehydrogenase from the psychrophilic bacterium strain PA-43: overexpression, molecular characterization, and sequence analysis (2003), Extremophiles, 7, 135-143.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.4.1.1 beta-mercaptoethanol slight activatory effect Shewanella sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.1.1 overexpression in Escherichia coli Shewanella sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.1 CaCl2 1 mM, 15% inhibition Shewanella sp.
1.4.1.1 Cibacron blue F3GA 0.1 mM, 70% inhibition Shewanella sp.
1.4.1.1 DTNB 7% inhibition with 0.01 M and 40% inhibition with 0.1 mM Shewanella sp.
1.4.1.1 L-Cys 10 mM, 40% inhibition Shewanella sp.
1.4.1.1 MnCl2 1 mM, 10% inhibition Shewanella sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.1 0.04
-
 NAD+ pH 10.0, 10°C or 15°C Shewanella sp.
1.4.1.1 0.04
-
 NADH pH 10.0, 5°C Shewanella sp.
1.4.1.1 0.05
-
 NAD+ pH 10.0, 25°C Shewanella sp.
1.4.1.1 0.07
-
 NAD+ pH 10.0, 35°C Shewanella sp.
1.4.1.1 0.08
-
 NADH pH 10.0, 25°C or 40°C Shewanella sp.
1.4.1.1 0.1
-
 NAD+ pH 10.0, 45°C Shewanella sp.
1.4.1.1 0.16
-
 NAD+ pH 10.0, 50°C Shewanella sp.
1.4.1.1 0.23
-
 NAD+ pH 10.0, 55°C Shewanella sp.
1.4.1.1 0.39
-
 pyruvate pH 10.0, 5°C Shewanella sp.
1.4.1.1 0.8
-
 pyruvate pH 10.0, 25°C Shewanella sp.
1.4.1.1 0.8
-
 pyruvate pH 10.0, 40°C Shewanella sp.
1.4.1.1 2 3 L-Ala pH 10.0, 55°C Shewanella sp.
1.4.1.1 9.7
-
 L-Ala pH 10.0, 25°C Shewanella sp.
1.4.1.1 10.1
-
 L-Ala pH 10.0, 10°C Shewanella sp.
1.4.1.1 10.6
-
 L-Ala pH 10.0, 15°C Shewanella sp.
1.4.1.1 10.8
-
 L-Ala pH 10.0, 45°C Shewanella sp.
1.4.1.1 25
-
 NH4+ pH 10.0, 25°C Shewanella sp.
1.4.1.1 28
-
 NH4+ pH 10.0, 5°C Shewanella sp.
1.4.1.1 30
-
 NH4+ pH 10.0, 40°C Shewanella sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.1 39596
-
 x * 39596, calculation from nucleotide sequence Shewanella sp.
1.4.1.1 260000
-
 gel filtration Shewanella sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.1 L-Ala + H2O + NAD+ Shewanella sp.
-
 pyruvate + NH3 + NADH
-
 ?
1.4.1.1 L-Ala + H2O + NAD+ Shewanella sp. PA-43
-
 pyruvate + NH3 + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.1 Shewanella sp. Q8RLH6
-
-
1.4.1.1 Shewanella sp. PA-43 Q8RLH6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.1
-
 Shewanella sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.1 24.8
-
-
 Shewanella sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.1 L-Ala + H2O + NAD+
-
 Shewanella sp. pyruvate + NH3 + NADH
-
 ?
1.4.1.1 L-Ala + H2O + NAD+
-
 Shewanella sp. PA-43 pyruvate + NH3 + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.1.1 ? x * 39596, calculation from nucleotide sequence Shewanella sp.

Synonyms

EC Number Synonyms Comment Organism
1.4.1.1 ALADH
-
 Shewanella sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.1 52
-
 half-life: 9 min Shewanella sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.1.1 19
-
 NAD+ pH 10.0, 10°C Shewanella sp.
1.4.1.1 41
-
 NAD+ pH 10.0, 15°C Shewanella sp.
1.4.1.1 45
-
 L-Ala pH 10.0, 10°C Shewanella sp.
1.4.1.1 63
-
 NAD+ pH 10.0, 25°C Shewanella sp.
1.4.1.1 67
-
 L-Ala pH 10.0, 15°C Shewanella sp.
1.4.1.1 75
-
 L-Ala pH 10.0, 25°C Shewanella sp.
1.4.1.1 128
-
 NAD+ pH 10.0, 35°C Shewanella sp.
1.4.1.1 163
-
 NAD+ pH 10.0, 55°C Shewanella sp.
1.4.1.1 207
-
 NAD+ pH 10.0, 45°C Shewanella sp.
1.4.1.1 211
-
 NAD+ pH 10.0, 50°C Shewanella sp.
1.4.1.1 215
-
 L-Ala pH 10.0, 55°C Shewanella sp.
1.4.1.1 228
-
 L-Ala pH 10.0, 45°C Shewanella sp.
1.4.1.1 888
-
 pyruvate pH 10.0, 5°C Shewanella sp.
1.4.1.1 977
-
 NH4+ pH 10.0, 5°C Shewanella sp.
1.4.1.1 1008
-
 NADH pH 10.0, 5°C Shewanella sp.
1.4.1.1 1755
-
 NH4+ pH 10.0, 25°C Shewanella sp.
1.4.1.1 1963
-
 pyruvate pH 10.0, 25°C Shewanella sp.
1.4.1.1 2209
-
 NADH pH 10.0, 25°C Shewanella sp.
1.4.1.1 2472
-
 NH4+ pH 10.0, 40°C Shewanella sp.
1.4.1.1 2562
-
 pyruvate pH 10.0, 40°C Shewanella sp.
1.4.1.1 2871
-
 NADH pH 10.0, 40°C Shewanella sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.1 NAD+
-
 Shewanella sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.4.1.1 Shewanella sp. theoretical value
-
 4.9