Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Sanon, A.; Lawrence, F.; Bories, C.; Loiseau, P.M.
    Purification of N-acetyl-beta-D-hexosaminidase from Trichomonas vaginalis (2001), Exp. Parasitol., 97, 169-172.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.52 Ag+ 1 mM, about 50% inhibition Trichomonas vaginalis
3.2.1.52 Cu2+ 1 mM, about 50% inhibition Trichomonas vaginalis
3.2.1.52 Hg2+ 1 mM, about 50% inhibition Trichomonas vaginalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.52 0.648
-
 p-nitrophenyl-N-acetyl-beta-D-glucosaminide
-
 Trichomonas vaginalis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.52 membrane
-
 Trichomonas vaginalis 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.52 204000
-
 non-denaturing PAGE Trichomonas vaginalis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.52 Trichomonas vaginalis
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.52 glycoprotein
-
 Trichomonas vaginalis

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.52
-
 Trichomonas vaginalis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.52 2.432
-
-
 Trichomonas vaginalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.52 p-nitrophenyl-N-acetyl-beta-D-glucosaminide + H2O
-
 Trichomonas vaginalis p-nitrophenol + N-acetyl-beta-D-glucosamine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.52 More SDS-PAGE yields a protein band of about 20000 Da, SDS-PAGE in presence of beta-mercaptoethanol yields protein bands of 64000 Da, 66000 Da and 72000 Da Trichomonas vaginalis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.52 50
-
 half-life: 225 min Trichomonas vaginalis
3.2.1.52 55
-
 40 min, over 70% of the initial activity is retained, half-life: 77 min Trichomonas vaginalis
3.2.1.52 65
-
 10 min, complete inactivation, half-life: below 3 min Trichomonas vaginalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.52 7
-
 in 200 mM potassium phosphate buffer Trichomonas vaginalis