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Literature summary extracted from

  • Soboh, B.; Linder, D.; Hedderich, R.
    Purification and catalytic properties of a CO-oxidizing:H2-evolving enzyme complex from Carboxydothermus hydrogenoformans (2002), Eur. J. Biochem., 269, 5712-5721.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.7.4 Ti(III)citrate 1mM for 1 min at 65°C, 45fold increase in initial activity Carboxydothermus hydrogenoformans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.7.4 CO 45% CO in headspace, 50% inhibition Carboxydothermus hydrogenoformans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.7.4 membrane
-
Carboxydothermus hydrogenoformans 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.7.4 Iron 61.5 mol nonheme iron per mol of complex Carboxydothermus hydrogenoformans
1.2.7.4 Nickel 3 mol per mol of complex Carboxydothermus hydrogenoformans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.7.4 62000
-
2 * 62000, enzyme, + electron transfer protein CooF and membrane bound [Ni-Fe] hydrogenase, six differentsubunits Carboxydothermus hydrogenoformans
1.2.7.4 62000
-
2 * 62000, SDS-PAGE, enzyme Carboxydothermus hydrogenoformans
1.2.7.4 120000
-
gel filtration, enzyme Carboxydothermus hydrogenoformans
1.2.7.4 450000
-
gel filtration, complex Carboxydothermus hydrogenoformans

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.4 Carboxydothermus hydrogenoformans
-
enzyme CooS in complex with an electron transfer protein CooF and a membrane bound [Ni-Fe] hydrogenase
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.7.4
-
Carboxydothermus hydrogenoformans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.7.4 5924
-
pH 7.0, 70°C Carboxydothermus hydrogenoformans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.4 CO + H2O + methyl viologen optimal concentration of CO 5% in headspace Carboxydothermus hydrogenoformans CO2 + reduced methyl viologen
-
?
1.2.7.4 H2 + reduced methyl viologen
-
Carboxydothermus hydrogenoformans methyl viologen
-
r

Subunits

EC Number Subunits Comment Organism
1.2.7.4 dimer 2 * 62000, SDS-PAGE, enzyme Carboxydothermus hydrogenoformans
1.2.7.4 octamer 2 * 62000, enzyme, + electron transfer protein CooF and membrane bound [Ni-Fe] hydrogenase, six differentsubunits Carboxydothermus hydrogenoformans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.7.4 6.5
-
both for enzyme and complex Carboxydothermus hydrogenoformans