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Literature summary extracted from

  • Bellevik, S.; Zhang, J.; Meijer, J.
    Brassica napus soluble epoxide hydrolase (BNSEH1) (2002), Eur. J. Biochem., 269, 5295-5302.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.3.2.10 agriculture the isolation of BNSEH1 will facilitate metabolic studies of resistance and seed oil modification in the important oilcrop Brassica napus Brassica napus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.3.2.10 expression in Pichia pastoris Brassica napus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.3.2.10 beta-naphthoflavone 1 mM, 50% inhibition Brassica napus
3.3.2.10 Chalcone oxide 1 mM, 98% inhibition Brassica napus
3.3.2.10 Dimethylsulfoxide
-
 Brassica napus
3.3.2.10 N,N'-dicyclohexylcarbodiimide 0.01 mM, complete inhibition Brassica napus
3.3.2.10 N,N'-dicyclohexylurea 0.01 mM, 30% inhibition Brassica napus
3.3.2.10 p-hydroxymercuribenzoate
-
 Brassica napus
3.3.2.10 quercetin 1 mM, 75% inhibition Brassica napus
3.3.2.10 tetrahydrofuran
-
 Brassica napus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.3.2.10 0.011
-
 trans-stilbene oxide pH 7.0, 30°C Brassica napus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.3.2.10 soluble
-
 Brassica napus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.3.2.10 37000
-
 1 * 37000, SDS-PAGE Brassica napus
3.3.2.10 45000
-
 gel filtration Brassica napus

Organism

EC Number Organism UniProt Comment Textmining
3.3.2.10 Brassica napus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.3.2.10 recombinant enzyme Brassica napus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.3.2.10 cis-stilbene oxide + H2O low activity Brassica napus 1,2-diphenylethane-1,2-diol
-
 ?
3.3.2.10 trans-stilbene oxide + H2O
-
 Brassica napus 1,2-diphenylethane-1,2-diol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.3.2.10 monomer 1 * 37000, SDS-PAGE Brassica napus

Synonyms

EC Number Synonyms Comment Organism
3.3.2.10 BNSEH1
-
 Brassica napus
3.3.2.10 SEH
-
 Brassica napus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.3.2.10 55
-
 reaction with trans-stilbene oxide Brassica napus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.3.2.10 30 55 30°C: about 35% of maximal activity, 55°C: maximum, 65°C: no activity, reaction with trans-stilbene oxide Brassica napus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.3.2.10 0.3
-
 trans-stilbene oxide pH 7.0, 30°C Brassica napus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.3.2.10 6 7 reaction with trans-stilbene oxide Brassica napus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.3.2.10 4.5 8 pH 4.5: about 40% of maximal activity, pH 8.0: about 50% of maximal activity, reaction with trans-stilbene oxide Brassica napus