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Literature summary extracted from

  • Boselli, A.; Sacchi, S.; Job, V.; Pilone, M.S.; Pollegioni, L.
    Role of tyrosine 238 in the active site of Rhodotorula gracilis D-amino acid oxidase. A site-directed mutagenesis study (2002), Eur. J. Biochem., 269, 4762-4771.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.4.3.3 Y238F 3fold slower turnover on D-Ala as substrate than wild-type enzyme. KM-value for D-Ala is 2fold lower than the wild-type value, Km-value for D-Ser is 4.7fold lower than the wild-type value, Km-value for D-Pro is 1.7fold lower than the wild-type value, Km-value for D-Trp is 1.5fold lower than the wild-type value, Km-value for cephalosporin is 2.6fold lower than the wild-type value, Km-value for D-Val is 3fold lower than the wild-type value, Km-value for D-Phe is 4.3fold lower than the wild-type value Rhodotorula toruloides
1.4.3.3 Y238S 3fold slower turnover on D-Ala as substrate than wild-type enzyme. KM-value for D-Ala is 2fold lower than the wild-type value, Km-value for D-Ser is 8fold lower than the wild-type value, Km-value for D-Pro is 1.6fold lower than the wild-type value, Km-value for D-Trp is identical to wild-type value, Km-value for cephalosporin is 2.6fold lower than the wild-type value, Km-value for D-Val is 3.2fold lower than the wild-type value, Km-value for D-Phe is 7.5fold lower than the wild-type value Rhodotorula toruloides

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.3 benzoate competitive Rhodotorula toruloides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.3 0.04
-
 D-Phe pH 8.5, 25°C, mutant enzyme Y238F Rhodotorula toruloides
1.4.3.3 0.07
-
 D-Phe pH 8.5, 25°C, mutant enzyme Y238S Rhodotorula toruloides
1.4.3.3 0.2
-
 D-Trp pH 8.5, 25°C, mutant enzyme Y238S Rhodotorula toruloides
1.4.3.3 0.26
-
 O2 pH 8.5, 25°C, mutant enzyme Y238F Rhodotorula toruloides
1.4.3.3 0.3
-
 D-Trp pH 8.5, 25°C, mutant enzyme Y238F Rhodotorula toruloides
1.4.3.3 0.3
-
 D-Phe pH 8.5, 25°C, wild-type enzyme Rhodotorula toruloides
1.4.3.3 0.3
-
 D-Trp pH 8.5, 25°C, wild-type enzyme Rhodotorula toruloides
1.4.3.3 0.96
-
 O2 pH 8.5, 25°C, mutant enzyme Y238S Rhodotorula toruloides
1.4.3.3 1.7
-
 D-Ser pH 8.5, 25°C, mutant enzyme Y238F Rhodotorula toruloides
1.4.3.3 1.9
-
 cephalosporin C pH 8.5, 25°C, mutant enzyme Y238F Rhodotorula toruloides
1.4.3.3 1.9
-
 cephalosporin C pH 8.5, 25°C, mutant enzyme Y238S Rhodotorula toruloides
1.4.3.3 2.3
-
 O2 pH 8.5, 25°C, wild-type enzyme Rhodotorula toruloides
1.4.3.3 2.6
-
 D-Ala pH 8.5, 25°C, wild-type enzyme Rhodotorula toruloides
1.4.3.3 2.9
-
 D-Ser pH 8.5, 25°C, mutant enzyme Y238S Rhodotorula toruloides
1.4.3.3 5
-
 cephalosporin C pH 8.5, 25°C, wild-type enzyme Rhodotorula toruloides
1.4.3.3 6
-
 D-Val pH 8.5, 25°C, mutant enzyme Y238F Rhodotorula toruloides
1.4.3.3 6.1
-
 D-Val pH 8.5, 25°C, mutant enzyme Y238S Rhodotorula toruloides
1.4.3.3 7.5
-
 D-Ala pH 8.5, 25°C, mutant enzyme Y238F Rhodotorula toruloides
1.4.3.3 7.8
-
 D-Ala pH 8.5, 25°C, mutant enzyme Y238S Rhodotorula toruloides
1.4.3.3 12.3
-
 D-Pro pH 8.5, 25°C, mutant enzyme Y238S Rhodotorula toruloides
1.4.3.3 13.5
-
 D-Pro pH 8.5, 25°C, mutant enzyme Y238F Rhodotorula toruloides
1.4.3.3 13.7
-
 D-Ser pH 8.5, 25°C, wild-type enzyme Rhodotorula toruloides
1.4.3.3 18.9
-
 D-Val pH 8.5, 25°C, wild-type enzyme Rhodotorula toruloides
1.4.3.3 21.5
-
 D-Pro pH 8.5, 25°C, wild-type enzyme Rhodotorula toruloides

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.3 Rhodotorula toruloides P80324
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.3
-
 Rhodotorula toruloides

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.3.3 37
-
-
 Rhodotorula toruloides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.3 cephalosporin C + H2O + O2
-
 Rhodotorula toruloides 7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-Ala + H2O + O2
-
 Rhodotorula toruloides pyruvate + NH3 + H2O2
-
 ?
1.4.3.3 D-Phe + H2O + O2
-
 Rhodotorula toruloides phenylpyruvate + NH3 + H2O2
-
 ?
1.4.3.3 D-Pro + H2O + O2
-
 Rhodotorula toruloides 2-oxopentanoate + NH3 + H2O2
-
 ?
1.4.3.3 D-Ser + H2O + O2
-
 Rhodotorula toruloides 2-oxo-3-hydroxypropionate + NH3 + H2O2
-
 ?
1.4.3.3 D-Trp + H2O + O2
-
 Rhodotorula toruloides indol-3-pyruvate + NH3 + H2O2
-
 ?
1.4.3.3 D-Val + H2O + O2
-
 Rhodotorula toruloides alpha-ketoisovalerate + NH3 + H2O2
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.3.3 120
-
 O2 pH 8.5, 25°C, mutant enzyme Y238S Rhodotorula toruloides
1.4.3.3 120
-
 D-Ala pH 8.5, 25°C, mutant enzyme Y238S Rhodotorula toruloides
1.4.3.3 125
-
 O2 pH 8.5, 25°C, mutant enzyme Y238F Rhodotorula toruloides
1.4.3.3 125
-
 D-Ala pH 8.5, 25°C, mutant enzyme Y238F Rhodotorula toruloides
1.4.3.3 350
-
 O2 pH 8.5, 25°C, wild-type enzyme Rhodotorula toruloides
1.4.3.3 350
-
 D-Ala pH 8.5, 25°C, wild-type enzyme Rhodotorula toruloides