EC Number | Cloned (Comment) | Organism |
---|---|---|
6.3.1.2 | mutant and native enzymes expressed in Escherichia coli | Phaseolus vulgaris |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
6.3.1.2 | D56A | complete loss of activity in reverse reaction, 83% of wild-type activity in forward reaction, salting out at much higher ammonium sulfate concentrations than wild type enzyme | Phaseolus vulgaris |
6.3.1.2 | D56E | complete loss of activity in reverse reaction, 65% of wild-type activity in forward reaction, salting out properties only slightly affected | Phaseolus vulgaris |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
6.3.1.2 | 2-mercaptoethanol | no inhibition of wild type enzyme, inhibits activity of D56A and D56E mutant enzymes | Phaseolus vulgaris | |
6.3.1.2 | cysteine | no inhibition of wild type enzyme, inhibits activity of D56A and D56E mutant enzymes | Phaseolus vulgaris | |
6.3.1.2 | dithioerythritol | no inhibition of wild type enzyme, inhibits activity of D56A and D56E mutant enzymes | Phaseolus vulgaris | |
6.3.1.2 | p-hydroxymercuribenzoate | complete inhibition of wild type and D56A mutant, 40% inhibition of D56E at 1 mM | Phaseolus vulgaris |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.1.2 | 0.013 | - |
NH4+ | D56E mutant enzyme, pH 7.5, 37°C | Phaseolus vulgaris | |
6.3.1.2 | 0.02 | - |
NH4+ | D56A mutant enzyme, pH 7.5, 37°C | Phaseolus vulgaris | |
6.3.1.2 | 0.025 | - |
NH4+ | wild type enzyme, pH 7.5, 37°C | Phaseolus vulgaris | |
6.3.1.2 | 0.3 | - |
ATP | wild type enzyme, pH 7.5, 37°C | Phaseolus vulgaris | |
6.3.1.2 | 1 | - |
ATP | D56E mutant enzyme, pH 7.5, 37°C | Phaseolus vulgaris | |
6.3.1.2 | 1.6 | - |
ATP | D56A mutant enzyme, pH 7.5, 37°C | Phaseolus vulgaris | |
6.3.1.2 | 7 | - |
L-glutamate | wild type enzyme, pH 7.5, 37°C | Phaseolus vulgaris | |
6.3.1.2 | 18 | - |
L-glutamate | D56E mutant enzyme, pH 7.5, 37°C | Phaseolus vulgaris | |
6.3.1.2 | 38 | - |
L-glutamate | D56A mutant enzyme, pH 7.5, 37°C | Phaseolus vulgaris |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
6.3.1.2 | 43000 | - |
SDS-PAGE | Phaseolus vulgaris |
6.3.1.2 | 350000 | - |
gel filtration | Phaseolus vulgaris |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.1.2 | ATP + glutamate + NH4+ | Phaseolus vulgaris | central enzyme of nitrogen metabolism, assimilation of ammonia | ADP + phosphate + L-glutamine | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.3.1.2 | Phaseolus vulgaris | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.3.1.2 | recombinant enzymes | Phaseolus vulgaris |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.1.2 | ATP + glutamate + NH4+ | - |
Phaseolus vulgaris | ADP + phosphate + L-glutamine | - |
r | |
6.3.1.2 | ATP + glutamate + NH4+ | central enzyme of nitrogen metabolism, assimilation of ammonia | Phaseolus vulgaris | ADP + phosphate + L-glutamine | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.3.1.2 | octamer | 8 * 43000, subunits by SDS-PAGE, native mass by gel filtration | Phaseolus vulgaris |
6.3.1.2 | tetramer | 4 * 43000, minor peak in gel filtration with a mass of 190000 detected for both mutant enzymes | Phaseolus vulgaris |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.3.1.2 | 37 | - |
forward reaction, wild-type and mutant enzymes | Phaseolus vulgaris |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.3.1.2 | 7.5 | - |
forward reaction, wild-type and mutant enzymes | Phaseolus vulgaris |