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Literature summary extracted from

  • Saito, T.; Hong, P.; Kato, K.; Okazaki, M.; Inagaki, H.; Maeda, S.; Yokogawa, Y.
    Purification and characterization of an extracellular laccase of a fungus (family Chaetomiaceae) isolated from soil (2003), Enzyme Microb. Technol., 33, 520-526.
No PubMed abstract available

Application

EC Number Application Comment Organism
1.10.3.2 degradation use of enzyme in biodegradation of endocrine-disrupting chemicals such as bisphenol A and nonylphenol Chaetomiaceae

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 dithiothreitol
-
 Chaetomiaceae
1.10.3.2 kojic acid
-
 Chaetomiaceae
1.10.3.2 L-cysteine
-
 Chaetomiaceae
1.10.3.2 p-coumaric acid
-
 Chaetomiaceae
1.10.3.2 thioglycolic acid
-
 Chaetomiaceae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 0.009
-
 syringaldazine pH 7.0, 30°C Chaetomiaceae
1.10.3.2 0.012
-
 2,6-dimethoxyphenol pH 7.0, 30°C Chaetomiaceae
1.10.3.2 0.016
-
 guaiacol pH 7.0, 30°C Chaetomiaceae
1.10.3.2 0.023
-
 pyrogallol pH 7.0, 30°C Chaetomiaceae
1.10.3.2 0.123
-
 hydroquinone pH 7.0, 30°C Chaetomiaceae
1.10.3.2 0.212
-
 N,N-dimethyl-1,4-phenylenediamine pH 7.0, 30°C Chaetomiaceae
1.10.3.2 0.223
-
 4-hydroxyindole pH 7.0, 30°C Chaetomiaceae
1.10.3.2 0.652
-
 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 7.0, 30°C Chaetomiaceae
1.10.3.2 1.05
-
 catechol pH 7.0, 30°C Chaetomiaceae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 copper 4 atoms per protein molecule, a type I CuII, a type II CuII and a type III binuclear CuII Chaetomiaceae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 73000
-
 1 * 73000, SDS-PAGE Chaetomiaceae
1.10.3.2 80000
-
 gel filtration Chaetomiaceae

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Chaetomiaceae
-
 fungus isolated from soil, growth in presence of caffeic acid
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2
-
 Chaetomiaceae

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.10.3.2 culture supernatant
-
 Chaetomiaceae
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Chaetomiaceae ? + H2O
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2
-
 Chaetomiaceae ? + H2O
-
 ?
1.10.3.2 4-hydroxyindole + O2
-
 Chaetomiaceae ? + H2O
-
 ?
1.10.3.2 catechol + O2
-
 Chaetomiaceae ? + H2O
-
 ?
1.10.3.2 guaiacol + O2
-
 Chaetomiaceae ? + H2O
-
 ?
1.10.3.2 hydroquinone + O2
-
 Chaetomiaceae ? + H2O
-
 ?
1.10.3.2 N,N-dimethyl-1,4-phenylenediamine + O2
-
 Chaetomiaceae ? + H2O
-
 ?
1.10.3.2 pyrogallol + O2
-
 Chaetomiaceae ? + H2O
-
 ?
1.10.3.2 syringaldazine + O2
-
 Chaetomiaceae ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 monomer 1 * 73000, SDS-PAGE Chaetomiaceae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 42
-
-
 Chaetomiaceae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 4
-
 stable up to 12 days Chaetomiaceae
1.10.3.2 25
-
 150h, 50% residaul activity Chaetomiaceae
1.10.3.2 40
-
 20h, 50% residual activity Chaetomiaceae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.10.3.2 3 6 guaiacol pH 7.0, 30°C Chaetomiaceae
1.10.3.2 4
-
 syringaldazine pH 7.0, 30°C Chaetomiaceae
1.10.3.2 10
-
 pyrogallol pH 7.0, 30°C Chaetomiaceae
1.10.3.2 18
-
 N,N-dimethyl-1,4-phenylenediamine pH 7.0, 30°C Chaetomiaceae
1.10.3.2 26
-
 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 7.0, 30°C Chaetomiaceae
1.10.3.2 34
-
 2,6-dimethoxyphenol pH 7.0, 30°C Chaetomiaceae
1.10.3.2 46
-
 catechol pH 7.0, 30°C Chaetomiaceae
1.10.3.2 55
-
 hydroquinone pH 7.0, 30°C Chaetomiaceae
1.10.3.2 128
-
 4-hydroxyindole pH 7.0, 30°C Chaetomiaceae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 7
-
-
 Chaetomiaceae

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.10.3.2 Chaetomiaceae isoelectric focusing
-
 3.5