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Literature summary extracted from

  • Martinez-Trujillo, A.; Perez-Avalos, O.; Ponce-Noyola, T.
    Enzymatic properties of a purified xylanase from mutant PN-120 of Cellulomonas flavigena (2003), Enzyme Microb. Technol., 32, 401-406.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.8 additional information
-
 additional information wild-type and mutant strain enzyme Cellulomonas flavigena

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.8 extracellular
-
 Cellulomonas flavigena
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 56000
-
 x * 56000, wild-type and mutant strain enzyme, SDS-PAGE Cellulomonas flavigena

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Cellulomonas flavigena
-
 and its mutant PN-120
-
3.2.1.8 Cellulomonas flavigena CDBB531
-
 and its mutant PN-120
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8 wild-type, 27.5fold, and mutant strain enzyme, 30.6fold, to homogeneity from culture supernatant Cellulomonas flavigena

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.8 322
-
 purified wild-type enzyme Cellulomonas flavigena
3.2.1.8 2500
-
 purified mutant enzyme Cellulomonas flavigena

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 xylan + H2O from birchwood Cellulomonas flavigena ?
-
 ?
3.2.1.8 xylan + H2O from birchwood Cellulomonas flavigena CDBB531 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.8 ? x * 56000, wild-type and mutant strain enzyme, SDS-PAGE Cellulomonas flavigena

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 xylanase
-
 Cellulomonas flavigena

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 50 55 wild-type enzyme Cellulomonas flavigena
3.2.1.8 55
-
 mutant enzyme Cellulomonas flavigena

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 6.5
-
 wild-type enzyme Cellulomonas flavigena
3.2.1.8 7
-
 mutant enzyme Cellulomonas flavigena

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.8 6.5 9 the mutant enzyme shows higher activity at pH 9.0 than the wild-type enzyme Cellulomonas flavigena

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.8 Cellulomonas flavigena isoelectric focusing, wild-type and mutant strain enzyme
-
 6