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Literature summary extracted from

  • Jung, H.; Xu, F.; Li, K.
    Purification and characterization of laccase from wood-degrading fungus Trichophyton rubrum LKY-7 (2002), Enzyme Microb. Technol., 30, 161-168.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 0.038
-
 methyl syringate pH 5.5, 20°C Trichophyton rubrum
1.10.3.2 0.045
-
 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 5.5, 20°C Trichophyton rubrum
1.10.3.2 2.3
-
 N-hydroxyacetanilide pH 5.5, 20°C Trichophyton rubrum
1.10.3.2 58
-
 violuric acid pH 5.5, 20°C Trichophyton rubrum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 65000
-
 x * 65000, SDS-PAGE Trichophyton rubrum

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Trichophyton rubrum
-
 growth on glucose-peptone medium
-
1.10.3.2 Trichophyton rubrum LKY-7
-
 growth on glucose-peptone medium
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2 purified enzyme has a distinct blue color Trichophyton rubrum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.10.3.2 culture supernatant
-
 Trichophyton rubrum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.2 300
-
 pH 5.5, 20°C Trichophyton rubrum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Trichophyton rubrum ? + H2O
-
 ?
1.10.3.2 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Trichophyton rubrum LKY-7 ? + H2O
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2 36% of activity with 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) Trichophyton rubrum ? + H2O
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2 36% of activity with 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) Trichophyton rubrum LKY-7 ? + H2O
-
 ?
1.10.3.2 4-methylcatechol + O2 18% of activity with 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) Trichophyton rubrum ? + H2O
-
 ?
1.10.3.2 4-methylcatechol + O2 18% of activity with 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) Trichophyton rubrum LKY-7 ? + H2O
-
 ?
1.10.3.2 catechol + O2 6.2% of activity with 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) Trichophyton rubrum ? + H2O
-
 ?
1.10.3.2 catechol + O2 6.2% of activity with 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) Trichophyton rubrum LKY-7 ? + H2O
-
 ?
1.10.3.2 ferulic acid + O2 9.4% of activity with 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) Trichophyton rubrum ? + H2O
-
 ?
1.10.3.2 ferulic acid + O2 9.4% of activity with 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) Trichophyton rubrum LKY-7 ? + H2O
-
 ?
1.10.3.2 guaiacol + O2 33% of activity with 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) Trichophyton rubrum ? + H2O
-
 ?
1.10.3.2 hydroquinone + O2 60% of activity with 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) Trichophyton rubrum ? + H2O
-
 ?
1.10.3.2 methyl syringate + O2
-
 Trichophyton rubrum ? + H2O
-
 ?
1.10.3.2 N-hydroxyacetanilide + O2
-
 Trichophyton rubrum ? + H2O
-
 ?
1.10.3.2 pyrogallol + O2 2% of activity with 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) Trichophyton rubrum ? + H2O
-
 ?
1.10.3.2 violuric acid + O2
-
 Trichophyton rubrum ? + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 ? x * 65000, SDS-PAGE Trichophyton rubrum
1.10.3.2 More N-terminal amino acid sequence Trichophyton rubrum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.10.3.2 5.5
-
 methyl syringate pH 5.5, 20°C Trichophyton rubrum
1.10.3.2 10
-
 violuric acid pH 5.5, 20°C Trichophyton rubrum
1.10.3.2 10.3
-
 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 5.5, 20°C Trichophyton rubrum
1.10.3.2 18.3
-
 N-hydroxyacetanilide pH 5.5, 20°C Trichophyton rubrum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 3
-
 substrate 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) Trichophyton rubrum
1.10.3.2 3.5
-
 substrate guaiacol Trichophyton rubrum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.10.3.2 3 4
-
 Trichophyton rubrum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.10.3.2 Trichophyton rubrum isoelectric focusing
-
 4