BRENDA - Enzyme Database

On the osmotic signal and osmosensing mechanism of an ABC transport system for glycine betaine

van der Heide, T.; Stuart, M.C.A.; Poolman, B.; EMBO J. 20, 7022-7032 (2001)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
7.6.2.9
anionic lipids
absence of phosphatidylglycerol and/or phosphatidylserine results in trapping of the protein in an inactive state
Lactococcus lactis
7.6.2.9
glycerol
at 270 mM, the amount of glycine betaine taken up after 30 seconds is 30% higher than that of the iso-osmotic control sample
Lactococcus lactis
7.6.2.9
additional information
sucrose and D-fructose have no effects on OpuA activity
Lactococcus lactis
7.6.2.9
phosphatidylethanolamine
non-bilayer lipid essential for high activity of OpuA
Lactococcus lactis
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
7.6.2.9
K3PO4
above 200 mM, inhibits ATPase activity
Lactococcus lactis
7.6.2.9
Tetracaine
low concentrations of the cationic amphipath decrease the activation of OpuA
Lactococcus lactis
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
7.6.2.9
membrane
-
Lactococcus lactis
16020
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
7.6.2.9
K2SO4
100 mosmol/kg osmolality, stimulates OpuA activity
Lactococcus lactis
7.6.2.9
K3PO4
100 mosmol/kg osmolality, pH 7.0, stimulates OpuA activity. 200 mM, stimulates ATPase activity
Lactococcus lactis
7.6.2.9
KCl
100 mM, activates OpuA instantaneous and reversible
Lactococcus lactis
7.6.2.9
Na2SO4
100 mosmol/kg osmolality, stimulates OpuA activity
Lactococcus lactis
7.6.2.9
NaCl
100 mosmol/kg osmolality, stimulates OpuA activity
Lactococcus lactis
7.6.2.9
Sodium phosphate
100 mosmol/kg osmolality, pH 7.0, stimulates OpuA activity
Lactococcus lactis
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
7.6.2.9
Lactococcus lactis
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
7.6.2.9
affinity chromatography of solubilized membranes
Lactococcus lactis
Reaction
EC Number
Reaction
Commentary
Organism
7.6.2.9
ATP + H2O + quaternary amine-[quaternary amine-binding protein][side 1] = ADP + phosphate + quaternary amine[side 2] + [quaternary amine-binding protein][side 1]
the osmotic activation of the transporter is set by the bulk charge in the lipid headgroup region of the membrane, indicating that electrostatic interactions between lipids and the transporter are intrinsic to the osmosensing mechanism
Lactococcus lactis
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
7.6.2.9
0.005
-
ATPase activity, pH 7.0, 200 mM K3PO4, 360 mosmol/kg
Lactococcus lactis
7.6.2.9
0.009
-
ATPase activity, pH 7.0, 50 mM K3PO4, 290 mM sucrose, 450 mosmol/kg
Lactococcus lactis
7.6.2.9
0.018
-
ATPase activity, pH 7.0, 50 mM K3PO4, 90 mosmol/kg
Lactococcus lactis
7.6.2.9
0.023
-
ATPase activity, pH 7.0, 100 mM K3PO4, 180 mosmol/kg
Lactococcus lactis
7.6.2.9
0.095
-
ATPase activity, pH 7.0, 50 mM K3PO4, 200 mM NaCl, 450 mosmol/kg
Lactococcus lactis
7.6.2.9
0.099
-
ATPase activity, pH 7.0, 50 mM K3PO4, 200 mM KCl, 450 mosmol/kg
Lactococcus lactis
7.6.2.9
0.117
-
ATPase activity, pH 7.0, 150 mM K3PO4, 270 mosmol/kg
Lactococcus lactis
7.6.2.9
0.124
-
ATPase activity, pH 7.0, 350 mM K3PO4, 630 mosmol/kg
Lactococcus lactis
7.6.2.9
0.146
-
ATPase activity, pH 7.0, 250 mM K3PO4, 450 mosmol/kg
Lactococcus lactis
7.6.2.9
0.148
-
ATPase activity, pH 7.0, 50 mM K3PO4, 160 mM K2SO4, 450 mosmol/kg
Lactococcus lactis
7.6.2.9
0.18
-
ATPase activity, pH 7.0, 200 mM K3PO4, 360 mosmol/kg
Lactococcus lactis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7.6.2.9
ATP + H2O + glycine betaine/out
-
655328
Lactococcus lactis
ADP + phosphate + glycine betaine/in
-
-
-
-
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
7.6.2.9
anionic lipids
absence of phosphatidylglycerol and/or phosphatidylserine results in trapping of the protein in an inactive state
Lactococcus lactis
7.6.2.9
glycerol
at 270 mM, the amount of glycine betaine taken up after 30 seconds is 30% higher than that of the iso-osmotic control sample
Lactococcus lactis
7.6.2.9
additional information
sucrose and D-fructose have no effects on OpuA activity
Lactococcus lactis
7.6.2.9
phosphatidylethanolamine
non-bilayer lipid essential for high activity of OpuA
Lactococcus lactis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
7.6.2.9
K3PO4
above 200 mM, inhibits ATPase activity
Lactococcus lactis
7.6.2.9
Tetracaine
low concentrations of the cationic amphipath decrease the activation of OpuA
Lactococcus lactis
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
7.6.2.9
membrane
-
Lactococcus lactis
16020
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
7.6.2.9
K2SO4
100 mosmol/kg osmolality, stimulates OpuA activity
Lactococcus lactis
7.6.2.9
K3PO4
100 mosmol/kg osmolality, pH 7.0, stimulates OpuA activity. 200 mM, stimulates ATPase activity
Lactococcus lactis
7.6.2.9
KCl
100 mM, activates OpuA instantaneous and reversible
Lactococcus lactis
7.6.2.9
Na2SO4
100 mosmol/kg osmolality, stimulates OpuA activity
Lactococcus lactis
7.6.2.9
NaCl
100 mosmol/kg osmolality, stimulates OpuA activity
Lactococcus lactis
7.6.2.9
Sodium phosphate
100 mosmol/kg osmolality, pH 7.0, stimulates OpuA activity
Lactococcus lactis
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
7.6.2.9
affinity chromatography of solubilized membranes
Lactococcus lactis
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
7.6.2.9
0.005
-
ATPase activity, pH 7.0, 200 mM K3PO4, 360 mosmol/kg
Lactococcus lactis
7.6.2.9
0.009
-
ATPase activity, pH 7.0, 50 mM K3PO4, 290 mM sucrose, 450 mosmol/kg
Lactococcus lactis
7.6.2.9
0.018
-
ATPase activity, pH 7.0, 50 mM K3PO4, 90 mosmol/kg
Lactococcus lactis
7.6.2.9
0.023
-
ATPase activity, pH 7.0, 100 mM K3PO4, 180 mosmol/kg
Lactococcus lactis
7.6.2.9
0.095
-
ATPase activity, pH 7.0, 50 mM K3PO4, 200 mM NaCl, 450 mosmol/kg
Lactococcus lactis
7.6.2.9
0.099
-
ATPase activity, pH 7.0, 50 mM K3PO4, 200 mM KCl, 450 mosmol/kg
Lactococcus lactis
7.6.2.9
0.117
-
ATPase activity, pH 7.0, 150 mM K3PO4, 270 mosmol/kg
Lactococcus lactis
7.6.2.9
0.124
-
ATPase activity, pH 7.0, 350 mM K3PO4, 630 mosmol/kg
Lactococcus lactis
7.6.2.9
0.146
-
ATPase activity, pH 7.0, 250 mM K3PO4, 450 mosmol/kg
Lactococcus lactis
7.6.2.9
0.148
-
ATPase activity, pH 7.0, 50 mM K3PO4, 160 mM K2SO4, 450 mosmol/kg
Lactococcus lactis
7.6.2.9
0.18
-
ATPase activity, pH 7.0, 200 mM K3PO4, 360 mosmol/kg
Lactococcus lactis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7.6.2.9
ATP + H2O + glycine betaine/out
-
655328
Lactococcus lactis
ADP + phosphate + glycine betaine/in
-
-
-
-