EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.27 | NAD+ | - |
Clupea harengus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.27 | 0.07 | - |
pyruvate | pH 7.2, 25°C, isoenzyme LDH-A2B2 | Clupea harengus | |
1.1.1.27 | 0.09 | - |
pyruvate | pH 7.6, 25°C, isoenzyme LDH-B4 | Clupea harengus | |
1.1.1.27 | 0.22 | - |
pyruvate | pH 7.1, 25°C, isoenzyme LDH-A4 | Clupea harengus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.27 | Clarias gariepinus | - |
- |
- |
1.1.1.27 | Clupea harengus | - |
- |
- |
1.1.1.27 | Cyprinus carpio | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.27 | isoenzyme LDH-A4 from muscle | Clupea harengus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.1.27 | skeletal muscle | isoenzyme LDH-A4 | Clupea harengus | - |
1.1.1.27 | spermatozoon | - |
Cyprinus carpio | - |
1.1.1.27 | spermatozoon | - |
Clarias gariepinus | - |
1.1.1.27 | spermatozoon | isoenzyme LDH-B4 and LDH-A2B2 | Clupea harengus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.27 | pyruvate + NADH + H+ | - |
Cyprinus carpio | (S)-lactate + NAD+ | - |
? | |
1.1.1.27 | pyruvate + NADH + H+ | - |
Clarias gariepinus | (S)-lactate + NAD+ | - |
? | |
1.1.1.27 | pyruvate + NADH + H+ | - |
Clupea harengus | (S)-lactate + NAD+ | - |
? |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.27 | 45 | - |
20 min, about 10% loss of activity, isoenzyme A4 | Clupea harengus |
1.1.1.27 | 50 | - |
20 min, about 40% loss of activity of isoenzyme A4, about 10% loss of activity of isoenzyme B4, about 25% loss of activity of isoenzyme A2B2 | Clupea harengus |
1.1.1.27 | 51 | - |
20 min, about 75% loss of activity, isoenzyme A4 | Clupea harengus |
1.1.1.27 | 53 | - |
20 min, complete loss of activity of isoenzyme A4, about 40% loss of activity of isoenzyme A2B2 | Clupea harengus |
1.1.1.27 | 55 | - |
20 min, about 90% loss of activity of isoenzyme A2B2, about 15% loss of activity of isoenzyme B4 | Clupea harengus |
1.1.1.27 | 66 | - |
20 min, about 35% loss of activity of isoenzyme B4 | Clupea harengus |
1.1.1.27 | 68 | - |
20 min, about 68% loss of activity of isoenzyme B4 | Clupea harengus |
1.1.1.27 | 71 | - |
20 min, complete loss of activity of isoenzyme B4 | Clupea harengus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.27 | 7.1 | - |
reduction of pyruvate, isoenzyme LDH-A4 | Clupea harengus |
1.1.1.27 | 7.25 | - |
reduction of pyruvate, isoenzyme LDH-A2B2 | Clupea harengus |
1.1.1.27 | 7.6 | - |
reduction of pyruvate, isoenzyme LDH-B4 | Clupea harengus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.27 | 5.5 | 8 | pH 5.5: 40% of maximal activity of isoenzyme LDH-B4 and 60% of maximal activity of isoenzyme LDH-A2B2 and LDH-A4, pH 8.0: about 60% of maximal activity of isoenzyme LDH-A4, LDH-B4 and LDH-A4B4, reduction of pyruvate | Clupea harengus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.27 | NADH | - |
Cyprinus carpio | |
1.1.1.27 | NADH | - |
Clarias gariepinus | |
1.1.1.27 | NADH | - |
Clupea harengus |