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Literature summary extracted from

  • Usami, N.; Ishikura, S.; Abe, H.; Nagano, M.; Uebuchi, M.; Kuniyasu, A.; Otagiri, M.; Nakayama, H.; Imamura, Y.; Hara, A.
    Cloning, expression and tissue distribution of a tetrameric form of pig carbonyl reductase (2003), Chem. Biol. Interact., 143-144, 353-361.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.184 DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.184 genistein 50% inhibition at 0.028 mM Sus scrofa
1.1.1.184 kaempferol 50% inhibition at 0.015 mM Sus scrofa
1.1.1.184 myristic acid 50% inhibition at 0.025 mM Sus scrofa
1.1.1.184 quercetin 50% inhibition at 0.016 mM Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.184 0.002
-
 9,10-phenanthrenequinone pH 6.0, recombinant enzyme Sus scrofa
1.1.1.184 0.004
-
 1-phenylisatin pH 6.0, recombinant enzyme Sus scrofa
1.1.1.184 0.021
-
 hexanophenone pH 6.0, recombinant enzyme Sus scrofa
1.1.1.184 0.028
-
 heptanophenone pH 6.0, recombinant enzyme Sus scrofa
1.1.1.184 0.03
-
 isatin pH 6.0, recombinant enzyme Sus scrofa
1.1.1.184 0.031
-
 nonanophenone pH 6.0, recombinant enzyme Sus scrofa
1.1.1.184 0.04
-
 1-phenyl-1,2-propanedione pH 6.0, recombinant enzyme Sus scrofa
1.1.1.184 0.052
-
 valerophenone pH 6.0, recombinant enzyme Sus scrofa
1.1.1.184 0.086
-
 16-ketoestrone pH 6.0, recombinant enzyme Sus scrofa
1.1.1.184 0.09
-
 4-hexanoylpyridine pH 6.0, recombinant enzyme Sus scrofa
1.1.1.184 0.13
-
 benzil pH 6.0, recombinant enzyme Sus scrofa
1.1.1.184 0.17
-
 n-butyrophenone pH 6.0, recombinant enzyme Sus scrofa
1.1.1.184 0.29
-
 menadione pH 6.0, recombinant enzyme Sus scrofa
1.1.1.184 0.29
-
 4-benzoylpyridine pH 6.0, recombinant enzyme Sus scrofa
1.1.1.184 0.47
-
 2,3-hexandione pH 6.0, recombinant enzyme Sus scrofa
1.1.1.184 0.76
-
 3-benzoylpyridine pH 6.0, recombinant enzyme Sus scrofa
1.1.1.184 0.89
-
 propiophenone pH 6.0, recombinant enzyme Sus scrofa
1.1.1.184 1.4
-
 Pyridine-4-aldehyde pH 6.0, recombinant enzyme Sus scrofa
1.1.1.184 4.5
-
 diacetyl pH 6.0, recombinant enzyme Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.184 27000
-
 4 * 27000, SDS-PAGE Sus scrofa
1.1.1.184 100000
-
 about, gel filtration Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.184 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.184 recombinant enzyme from Escherichia coli Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.184 heart high expression level Sus scrofa
-
1.1.1.184 intestine
-
 Sus scrofa
-
1.1.1.184 kidney high expression level Sus scrofa
-
1.1.1.184 liver
-
 Sus scrofa
-
1.1.1.184 additional information tissue distribution, overview Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.184 0.5
-
 purified recombinant enzyme Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.184 1-phenyl-1,2-propanedione + NADPH recombinant enzyme Sus scrofa ? + NADP+
-
 ?
1.1.1.184 1-phenylisatin + NADPH high activity, recombinant enzyme Sus scrofa ? + NADP+
-
 ?
1.1.1.184 16-ketoestrone + NADPH recombinant enzyme Sus scrofa ? + NADP+
-
 r
1.1.1.184 2,3-hexadione + NADPH recombinant enzyme Sus scrofa ? + NADP+
-
 ?
1.1.1.184 3-benzoylpyridine + NADPH recombinant enzyme Sus scrofa (S)-alpha-phenyl-3-pyridylmethanol + NADP+
-
 ?
1.1.1.184 4-benzoylpyridine + NADPH + H+ recombinant enzyme Sus scrofa (S)-alpha-phenyl-4-pyridylmethanol + NADP+
-
 ?
1.1.1.184 4-hexanoylpyridine + NADPH + H+ recombinant enzyme Sus scrofa 1-(pyridin-4-yloxy)hexan-1-ol + NADP+
-
 ?
1.1.1.184 9,10-phenanthrenequinone + NADPH best substrate, recombinant enzyme Sus scrofa ? + NADP+
-
 r
1.1.1.184 9-cis-retinal + NADPH recombinant enzyme Sus scrofa 9-cis-retinol + NADP+
-
 ?
1.1.1.184 all-trans-retinal + NADPH + H+ recombinant enzyme Sus scrofa all-trans-retinol + NADP+
-
 ?
1.1.1.184 benzil + NADPH recombinant enzyme Sus scrofa ? + NADP+
-
 ?
1.1.1.184 diacetyl + NADPH recombinant enzyme Sus scrofa ? + NADP+
-
 ?
1.1.1.184 heptanophenone + NADPH recombinant enzyme Sus scrofa 1-phenylheptan-1-ol + NADP+
-
 ?
1.1.1.184 hexanophenone + NADPH recombinant enzyme Sus scrofa 1-phenylhexan-1-ol + NADP+
-
 ?
1.1.1.184 isatin + NADPH recombinant enzyme Sus scrofa ? + NADP+
-
 r
1.1.1.184 menadione + NADPH recombinant enzyme Sus scrofa ? + NADP+
-
 ?
1.1.1.184 additional information substrate specificity, overview Sus scrofa ?
-
 ?
1.1.1.184 n-butyrophenone + NADPH recombinant enzyme Sus scrofa 1-phenylbutan-1-ol + NADP+
-
 ?
1.1.1.184 nonanophenone + NADPH recombinant enzyme Sus scrofa 1-phenylnonan-1-ol + NADP+
-
 ?
1.1.1.184 propiophenone + NADPH recombinant enzyme Sus scrofa 1-phenylpropan-1-ol + NADP+
-
 ?
1.1.1.184 pyridine-4-aldehyde + NADPH + H+ recombinant enzyme Sus scrofa pyridin-4-ylmethanol + NADP+
-
 ?
1.1.1.184 valerophenone + NADPH recombinant enzyme Sus scrofa 1-phenylpentan-1-ol + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.184 tetramer 4 * 27000, SDS-PAGE Sus scrofa

Synonyms

EC Number Synonyms Comment Organism
1.1.1.184 carbonyl reductase
-
 Sus scrofa
1.1.1.184 CR
-
 Sus scrofa
1.1.1.184 PHCR
-
 Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.184 5.5
-
 about Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.184 NADPH
-
 Sus scrofa