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Literature summary extracted from
- Hydrolysis of low-molecular-weight oligosaccharides and oligosaccharide alditols by pig intestinal sucrase/isomaltase and glucosidase/maltase (2000), Carbohydr. Res., 326, 264-276.
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.48 | brush border | - |
Sus scrofa | 5903 | - |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.10 | Sus scrofa | - |
enzyme SI, bifunctional enzyme with sucrase and isomaltase activity | - |
| 3.2.1.48 | Sus scrofa | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.48 | - |
Sus scrofa |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 3.2.1.48 | isomaltose + h2O = 2 D-glucose | The enzyme complex consists of two noncovalently linked subunits. The active site of the sucrase subunit cleaves alpha,beta-(1-2)glycosidic bonds and contains only two subsites and, thus can bind only two monomeric parts of a substrate with positive affinity. The isomaltase cleaves alpha-(1-6) and alpha-(1-1) glycoside bonds | Sus scrofa |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.10 | intestine | - |
Sus scrofa | - |
| 3.2.1.48 | intestinal mucosa | - |
Sus scrofa | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.10 | 1,6-di-O-alpha-D-glucitol + H2O | - |
Sus scrofa | D-glucitol | - |
? |  |
| 3.2.1.10 | 1,6-di-O-alpha-D-glucopyranosyl-D-fructofuranose + H2O | - |
Sus scrofa | D-glucose + D-fructose | - |
? | |
| 3.2.1.10 | 1,6-di-O-alpha-D-mannitol + H2O | - |
Sus scrofa | D-mannitol | - |
? | |
| 3.2.1.10 | 1-O-alpha-D-glucopyranosyl-D-fructopyranose + H2O | - |
Sus scrofa | D-fructose | - |
? | |
| 3.2.1.10 | 1-O-alpha-D-glucopyranosyl-D-glucitol + H2O | - |
Sus scrofa | D-glucose + D-glucitol | - |
? | |
| 3.2.1.10 | 1-O-alpha-D-glucopyranosyl-D-mannitol + H2O | - |
Sus scrofa | D-glucose + D-mannitol | - |
? | |
| 3.2.1.10 | 4-O-alpha-D-glucopyranosyl-D-glucopyranose + H2O | - |
Sus scrofa | D-glucose | - |
? | |
| 3.2.1.10 | 6-O-alpha-D-glucopyranosyl-D-fructofuranose + H2O | - |
Sus scrofa | D-glucose + D-fructose | - |
? | |
| 3.2.1.10 | 6-O-alpha-D-glucopyranosyl-D-glucitol + H2O | - |
Sus scrofa | D-glucose + D-glucitol | - |
? | |
| 3.2.1.10 | 6-O-alpha-D-glucopyranosyl-D-glucopyranose + H2O | - |
Sus scrofa | D-glucose | - |
? | |
| 3.2.1.10 | alpha-D-glucopyranosyl-(1-6)-alpha-D-glucopyranosyl-(1-1)-D-glucitol + H2O | - |
Sus scrofa | D-glucose + D-glucitol | - |
? | |
| 3.2.1.10 | alpha-D-glucopyranosyl-(1-6)-alpha-D-glucopyranosyl-(1-1)-D-mannitol + H2O | - |
Sus scrofa | D-glucose + D-mannitol | - |
? | |
| 3.2.1.10 | alpha-D-glucopyranosyl-(1-6)-alpha-D-glucopyranosyl-(1-6)-alpha-D-glucopyranosyl-(1-1)-D-mannitol + H2O | - |
Sus scrofa | D-glucose + D-mannitol | - |
? | |
| 3.2.1.10 | alpha-D-glucopyranosyl-(1-6)-alpha-D-glucopyranosyl-(1-6)-alpha-D-glucopyranosyl-(1-6)-D-glucitol + H2O | - |
Sus scrofa | D-glucose + D-glucitol | - |
? | |
| 3.2.1.10 | alpha-D-glucopyranosyl-(1-6)-alpha-D-glucopyranosyl-(1-6)-alpha-D-glucopyranosyl-D-fructofuranose + H2O | - |
Sus scrofa | D-glucose + D-fructose | - |
? | |
| 3.2.1.10 | alpha-D-glucopyranosyl-(1-6)-alpha-D-glucopyranosyl-(1-6)-D-fructofuranose + H2O | - |
Sus scrofa | D-glucose + D-fructose | - |
? | |
| 3.2.1.10 | alpha-D-glucopyranosyl-(1-6)-alpha-D-glucopyranosyl-(1-6)-D-glucitol + H2O | - |
Sus scrofa | D-glucose + D-glucitol | - |
? | |
| 3.2.1.10 | alpha-D-glucopyranosyl-(1-6)-alpha-D-glucopyranosyl-(1-6)-D-glucopyranose + H2O | - |
Sus scrofa | D-glucose | - |
? | |
| 3.2.1.10 | alpha-D-glucopyranosyl-(1-6)-alpha-D-glucopyranosyl-D-fructofuranose + H2O | - |
Sus scrofa | D-glucose + D-fructose | - |
? | |
| 3.2.1.10 | alpha-D-glucopyranosyl-(1-6)-beta-fructofuranosyl-alpha-D-glucopyranoside + H2O | - |
Sus scrofa | D-glucose + D-fructose | - |
? | |
| 3.2.1.10 | additional information | substrate specificities of the 2 enzyme activities with the different substrate variants, overview, the active site of the sucrase subunit cleaves alpha,beta(1-2)-glycosidic bonds, and only 2 monomer units of the substrates bind with favourable affinity, while the isomaltase subunit possesses more than 2 subsites and cleaves oligosaccharides and reduced oligosaccharides with alpha(1-6)- and alpha(1-1)-glycosidic bonds, the isomaltase has binding sites for various aglycons | Sus scrofa | ? | - |
? | |
| 3.2.1.48 | 1,6-di-O-alpha-D-glucopyranosyl-D-fructofuranose + H2O | 99% cleavage compared to 1,6-di-O-alpha-D-glucopyranosyl-D-fructofuranose | Sus scrofa | alpha-D-glucose + D-fructose | - |
? | |
| 3.2.1.48 | 1-O-alpha-D-glucopyranosyl-D-glucitol + H2O | 68% cleavage compared to 1-O-alpha-D-glucopyranosyl-D-glucitol | Sus scrofa | alpha-D-glucose + D-glucitol | - |
? | |
| 3.2.1.48 | 1-O-alpha-D-glucopyranosyl-D-mannitol + H2O | 25% cleavage compared to 1-O-alpha-D-glucopyranosyl-D-mannitol | Sus scrofa | alpha-D-glucose + D-mannitol | - |
? | |
| 3.2.1.48 | 6-O-alpha-D-glucopyranosyl-D-glucitol + H2O | 35% cleavage compared to 6-O-alpha-D-glucopyranosyl-D-glucitol | Sus scrofa | alpha-D-glucose + D-glucitol | - |
? | |
| 3.2.1.48 | alpha-D-glucopyranosyl-(1-6)-alpha-D-glucopyranosyl-(1-6)-D-fructofuranose + H2O | 97% cleavage compared to alpha-D-glucopyranosyl-(1-6)-alpha-D-glucopyranosyl-(1-6)-D-fructofuranose | Sus scrofa | alpha-D-glucose + D-fructose | - |
? | |
| 3.2.1.48 | alpha-D-glucopyranosyl-(1-6)-alpha-D-glucopyranosyl-(1-6)-D-fructopyranose + H2O | 92% cleavage compared to alpha-D-glucopyranosyl-(1-6)-alpha-D-glucopyranosyl-(1-6)-D-fructopyranose | Sus scrofa | alpha-D-glucose + D-fructose | - |
? | |
| 3.2.1.48 | alpha-D-glucopyranosyl-(1-6)-alpha-D-glucopyranosyl-(1-6)-D-glucopyranose + H2O | - |
Sus scrofa | alpha-D-glucose | - |
? | |
| 3.2.1.48 | alpha-D-glucopyranosyl-(1-6)-alpha-D-glucopyranosyl-(1-6)-D-glucopyranosyl-(1-6)-D-fructofuranose + H2O | 100% cleavage compared to alpha-D-glucopyranosyl-(1-6)-alpha-D-glucopyranosyl-(1-6)-D-glucopyranosyl-(1-6)-D-fructofuranose | Sus scrofa | alpha-D-glucose + D-fructose | - |
? | |
| 3.2.1.48 | alpha-D-glucopyranosyl-(1-6)-beta-fructofuranosyl-alpha-D-glucopyranoside + H2O | 29% cleavage compared to alpha-D-glucopyranosyl-(1-6)-beta-fructofuranosyl-alpha-D-glucopyranoside | Sus scrofa | alpha-D-glucose + beta-D-fructose | - |
? | |
| 3.2.1.48 | additional information | Additional substrates are mixtures of isomers containing mannitol and glucitol, e.g. alpha-D-glucopyranosyl-(1-6)-alpha-D-glucopyranosyl-(1-1)-D-mannitol and alpha-D-glucopyranosyl-(1-6)-alpha-D-glucopyranosyl-(1-1)-D-glucitol in a ratio 2:3, alpha-D-glucopyranosyl-(1-6)-alpha-D-glucopyranosyl-(1-6)-alpha-D-gluco-pyranosyl-(1-1)-D-mannitol and alpha-D-glucopyranosyl-(1-6)-alpha-D-glucopyranosyl-(1-6)-alpha-D-gluco-pyranosyl-(1-1)-D-glucitol in a ratio 2:3, 1,6-di-O-alpha-D-glucopyranosyl-D-mannitol and 1,6-di-O-alpha-D-glucopyranosyl-D-glucitol, alpha-D-glucopyranosyl-(1-6)-alpha-D-glucopyranosyl-(1-1)-D-mannitol and alpha-D-glucopyranosyl-(1-6)-alpha-D-glucopyranosyl-(1-1)-D-glucitol. The end products are glucose, mannose and glucitol in accordance with the composition of the initial substrate. | Sus scrofa | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.10 | intestinal sucrase/isomaltase | - |
Sus scrofa |
| 3.2.1.10 | SI | - |
Sus scrofa |
| 3.2.1.48 | sucrase/isomaltase | - |
Sus scrofa |
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