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Literature summary extracted from

  • Petrova, S.D.; Ilieva, S.Z.; Bakalova, N.G.; Atev, A.P.; Bhat, M.K.; Kolev, D.N.
    Production and characterization of extracellular alpha-amylases from the thermophilic fungus Thermomyces lanuginosus (wild and mutant strains) (2000), Biotechnol. Lett., 22, 1619-1624.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.1 1-cyclohexyl-3-(morpholinyl-4-ethyl)-carbodiimide 40 mM, 50% inhibition after 20 min, 100 mM, 44% inhibition after 10 min in the presence of 1% starch Thermomyces lanuginosus
3.2.1.1 Cu2+ 5 mM, 90% inhibition Thermomyces lanuginosus
3.2.1.1 Fe3+ 5 mM, 90% inhibition Thermomyces lanuginosus
3.2.1.1 N-bromosuccinimide 1 mM, complete inhibition after 5 min Thermomyces lanuginosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.1 additional information
-
starch 0.5 mg/ml, 50°C, pH 5.0 Thermomyces lanuginosus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.1 Ba2+ activation Thermomyces lanuginosus
3.2.1.1 Ca2+ activation Thermomyces lanuginosus
3.2.1.1 Cd2+ activation Thermomyces lanuginosus
3.2.1.1 Mg2+ activation Thermomyces lanuginosus
3.2.1.1 Mn2+ activation Thermomyces lanuginosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.1 58000
-
gel filtration Thermomyces lanuginosus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.1 starch + H2O Thermomyces lanuginosus potato starch maltose + maltotriose major products ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.1 Thermomyces lanuginosus
-
strain ATCC 34626
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.1 2-propanol, Mono Q, Superose 12 Thermomyces lanuginosus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.1 349
-
-
Thermomyces lanuginosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.1 amylopectin + H2O 55% of activity with potato starch Thermomyces lanuginosus maltooligosaccharides
-
?
3.2.1.1 amylose + H2O 73% of activity with potato starch Thermomyces lanuginosus maltooligosaccharides
-
?
3.2.1.1 beta-cyclodextrin + H2O 27% of activity with potato starch Thermomyces lanuginosus maltooligosaccharides
-
?
3.2.1.1 maltodextrin + H2O 100% of activity with potato starch Thermomyces lanuginosus maltooligosaccharides
-
?
3.2.1.1 maltoheptaose + H2O 43% of activity with potato starch Thermomyces lanuginosus maltohexaose + maltopentaose + maltotetraose + maltotriose + maltose
-
?
3.2.1.1 maltohexaose + H2O 45% of activity with potato starch Thermomyces lanuginosus maltopentaose + maltotetraose + maltotriose + maltose
-
?
3.2.1.1 maltooligosaccharides + H2O 11% of activity with potato starch Thermomyces lanuginosus maltohexaose + maltopentaose + maltotetraose + maltotriose + maltose
-
?
3.2.1.1 maltopentaose + H2O 26% of activity with potato starch Thermomyces lanuginosus maltotetraose + maltotriose + maltose
-
?
3.2.1.1 maltotetraose + H2O 46% of activity with potato starch Thermomyces lanuginosus maltotriose + maltose
-
?
3.2.1.1 maltotriose + H2O 6% of activity with potato starch Thermomyces lanuginosus maltose + D-glucose
-
?
3.2.1.1 starch + H2O potato starch Thermomyces lanuginosus maltose + maltotriose major products ?

Subunits

EC Number Subunits Comment Organism
3.2.1.1 monomer 1 * 58000, SDS-PAGE Thermomyces lanuginosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.1 70
-
-
Thermomyces lanuginosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.1 50
-
32% and 94% loss of activity after 2 h and 18 h, respectively Thermomyces lanuginosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.1 5
-
-
Thermomyces lanuginosus