EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.1 | 1-cyclohexyl-3-(morpholinyl-4-ethyl)-carbodiimide | 40 mM, 50% inhibition after 20 min, 100 mM, 44% inhibition after 10 min in the presence of 1% starch | Thermomyces lanuginosus | |
3.2.1.1 | Cu2+ | 5 mM, 90% inhibition | Thermomyces lanuginosus | |
3.2.1.1 | Fe3+ | 5 mM, 90% inhibition | Thermomyces lanuginosus | |
3.2.1.1 | N-bromosuccinimide | 1 mM, complete inhibition after 5 min | Thermomyces lanuginosus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.1 | additional information | - |
starch | 0.5 mg/ml, 50°C, pH 5.0 | Thermomyces lanuginosus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.1 | Ba2+ | activation | Thermomyces lanuginosus | |
3.2.1.1 | Ca2+ | activation | Thermomyces lanuginosus | |
3.2.1.1 | Cd2+ | activation | Thermomyces lanuginosus | |
3.2.1.1 | Mg2+ | activation | Thermomyces lanuginosus | |
3.2.1.1 | Mn2+ | activation | Thermomyces lanuginosus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.1 | 58000 | - |
gel filtration | Thermomyces lanuginosus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.1 | starch + H2O | Thermomyces lanuginosus | potato starch | maltose + maltotriose | major products | ? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.1 | Thermomyces lanuginosus | - |
strain ATCC 34626 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.1 | 2-propanol, Mono Q, Superose 12 | Thermomyces lanuginosus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.1 | 349 | - |
- |
Thermomyces lanuginosus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.1 | amylopectin + H2O | 55% of activity with potato starch | Thermomyces lanuginosus | maltooligosaccharides | - |
? | |
3.2.1.1 | amylose + H2O | 73% of activity with potato starch | Thermomyces lanuginosus | maltooligosaccharides | - |
? | |
3.2.1.1 | beta-cyclodextrin + H2O | 27% of activity with potato starch | Thermomyces lanuginosus | maltooligosaccharides | - |
? | |
3.2.1.1 | maltodextrin + H2O | 100% of activity with potato starch | Thermomyces lanuginosus | maltooligosaccharides | - |
? | |
3.2.1.1 | maltoheptaose + H2O | 43% of activity with potato starch | Thermomyces lanuginosus | maltohexaose + maltopentaose + maltotetraose + maltotriose + maltose | - |
? | |
3.2.1.1 | maltohexaose + H2O | 45% of activity with potato starch | Thermomyces lanuginosus | maltopentaose + maltotetraose + maltotriose + maltose | - |
? | |
3.2.1.1 | maltooligosaccharides + H2O | 11% of activity with potato starch | Thermomyces lanuginosus | maltohexaose + maltopentaose + maltotetraose + maltotriose + maltose | - |
? | |
3.2.1.1 | maltopentaose + H2O | 26% of activity with potato starch | Thermomyces lanuginosus | maltotetraose + maltotriose + maltose | - |
? | |
3.2.1.1 | maltotetraose + H2O | 46% of activity with potato starch | Thermomyces lanuginosus | maltotriose + maltose | - |
? | |
3.2.1.1 | maltotriose + H2O | 6% of activity with potato starch | Thermomyces lanuginosus | maltose + D-glucose | - |
? | |
3.2.1.1 | starch + H2O | potato starch | Thermomyces lanuginosus | maltose + maltotriose | major products | ? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.1 | monomer | 1 * 58000, SDS-PAGE | Thermomyces lanuginosus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.1 | 70 | - |
- |
Thermomyces lanuginosus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.1 | 50 | - |
32% and 94% loss of activity after 2 h and 18 h, respectively | Thermomyces lanuginosus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.1 | 5 | - |
- |
Thermomyces lanuginosus |