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Literature summary extracted from

  • Chowdhury, E.K.; Akaishi, Y.; Nagata, S.; Misono, H.
    Cloning and overexpression of the 3-hydroxyisobutyrate dehydrogenase gene from Pseudomonas putida E23 (2003), Biosci. Biotechnol. Biochem., 67, 438-441.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.31
-
 Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.31 30280
-
 x * 30280, deduced from gene sequence, x * 30300, SDS-PAGE Pseudomonas putida
1.1.1.31 30300
-
 x * 30280, deduced from gene sequence, x * 30300, SDS-PAGE Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.31 Pseudomonas putida Q76KL5 recombinant enzyme expressed in Escherichia coli
-
1.1.1.31 Pseudomonas putida E23 Q76KL5 recombinant enzyme expressed in Escherichia coli
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.31
-
 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.31 L-3-hydroxyisobutyrate + NAD+ high stereospecificity Pseudomonas putida 3-oxoisobutyrate + NADH
-
 r
1.1.1.31 L-3-hydroxyisobutyrate + NAD+ high stereospecificity Pseudomonas putida E23 3-oxoisobutyrate + NADH
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.31 ? x * 30280, deduced from gene sequence, x * 30300, SDS-PAGE Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.31 NAD+
-
 Pseudomonas putida