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Literature summary extracted from

  • Yanai, T.; Sato, M.
    Purification and characterization of an alpha-L-rhamnosidase from Pichia angusta X349 (2000), Biosci. Biotechnol. Biochem., 64, 2179-2185.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.40 biotechnology enzyme efficiently releases monoterpenols from an aroma precursor from muscat grape juice Ogataea angusta

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.40 CuCl2 1 mM, 11% residual activity Ogataea angusta
3.2.1.40 D-glucose 500 mM, 76% residual activity Ogataea angusta
3.2.1.40 ethanol 12% v/v, 20% inhibition Ogataea angusta
3.2.1.40 HgCl2 1 mM, 11% residual activity Ogataea angusta
3.2.1.40 L-rhamnose competitive Ogataea angusta
3.2.1.40 additional information not inhibitory at 1 mM: EDTA, CaCl2, 2-mercaptoethanol Ogataea angusta
3.2.1.40 p-chloromercuribenzoate 1 mM, 11% residual activity Ogataea angusta

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.40 intracellular
-
 Ogataea angusta 5622
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.40 88000
-
 1 * 88000, SDS-PAGE Ogataea angusta
3.2.1.40 90000
-
 PAGE Ogataea angusta

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.40 Ogataea angusta
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.40
-
 Ogataea angusta

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.40 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Ogataea angusta 4-nitrophenol + D-glucopyranose 2.1% of activity compared to p-nitrophenyl-alpha-L-rhamnopyranoside ?
3.2.1.40 hesperidin + H2O 34% of activity compared to naringin Ogataea angusta ?
-
 ?
3.2.1.40 muscat glycoside extract
-
 Ogataea angusta linalool + alpha-terpineol + citronellol + nerol + geraniol + ?
-
 ?
3.2.1.40 naringin + H2O
-
 Ogataea angusta ?
-
 ?
3.2.1.40 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Ogataea angusta p-nitrophenol + alpha-L-arabinofuranose 6.1% of activity compared to p-nitrophenyl-alpha-L-rhamnopyranoside ?
3.2.1.40 p-nitrophenyl-alpha-L-rhamnopyranoside + H2O
-
 Ogataea angusta p-nitrophenol + alpha-L-rhamnopyranose
-
 ?
3.2.1.40 quercetin 3-alpha-L-rhamnopyranoside + H2O 31% of activity compared to naringin Ogataea angusta quercetin + L-rhamnose
-
 ?
3.2.1.40 rutin + H2O 63% of activity compared to naringin Ogataea angusta ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.40 monomer 1 * 88000, SDS-PAGE Ogataea angusta
3.2.1.40 More N-terminal sequence Ogataea angusta

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.40 40
-
-
 Ogataea angusta

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.40 30
-
 stable below Ogataea angusta

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.40 6
-
-
 Ogataea angusta

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.40 5 7
-
 Ogataea angusta

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.40 2 5 L-rhamnose pH 6.5, 30°C Ogataea angusta