Literature summary extracted from

Lu, X.; Nikolic, D.; Mitchell, D.J.; van Breemen, R.B.; Mersfelder, J.A.; Hille, R.; Silverman, R.B.
A mechanism for substrate-induced formation of 6-hydroxyflavin mononucleotide catalyzed by C30A trimethylamine dehydrogenase (2003), Bioorg. Med. Chem. Lett., 13, 4129-4132.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.5.8.2	expression of wild-type and mutant C30A in Escherichia coli strain JM109	Methylophilus methylotrophus

Protein Variants

EC Number	Protein Variants	Comment	Organism
1.5.8.2	C30A	mutant shows highly increased activity to oxidate of the cofactor FMN to 6-hydroxyFMN compared to the wild-type enzyme	Methylophilus methylotrophus

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
1.5.8.2	Fe2+	enzyme contains a [4Fe-4S] cluster	Methylophilus methylotrophus

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
1.5.8.2	trimethylamine + H2O + FMN	Methylophilus methylotrophus	-	dimethylamine + formaldehyde + FMNH2	-	r

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.5.8.2	Methylophilus methylotrophus	-	-	-

Reaction

EC Number	Reaction	Comment	Organism	Reaction ID
1.5.8.2	trimethylamine + H2O + electron-transfer flavoprotein = dimethylamine + formaldehyde + reduced electron-transfer flavoprotein	detailed reaction mechanism	Methylophilus methylotrophus	a

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.5.8.2	FMN + O2 + H2O	reaction mechanism, reoxidation of the cofactor, mutant C30A enzyme, low activity with wild-type enzyme and mutant W355L	Methylophilus methylotrophus	6-hydroxy-FMN + H2O2	the hydroxy group oxygen is derived from molecular oxygen not from water	r
1.5.8.2	trimethylamine + H2O + FMN	-	Methylophilus methylotrophus	dimethylamine + formaldehyde + FMNH2	-	r
1.5.8.2	trimethylamine + H2O + FMN	reaction mechanism	Methylophilus methylotrophus	dimethylamine + formaldehyde + FMNH2	-	r

Synonyms

EC Number	Synonyms	Comment	Organism
1.5.8.2	More	formerly EC 1.5.99.7	Methylophilus methylotrophus
1.5.8.2	TMADH	-	Methylophilus methylotrophus

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
1.5.8.2	7.7	-	assay at	Methylophilus methylotrophus

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.5.8.2	FMN	-	Methylophilus methylotrophus

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Release 2023.1 (January 2023)