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Literature summary extracted from

  • Ejim, L.; Mirza, I.A.; Capone, C.; Nazi, I.; Jenkins, S.; Chee, G.L.; Berghuis, A.M.; Wright, G.D.
    New phenolic inhibitors of yeast homoserine dehydrogenase (2004), Bioorg. Med. Chem., 12, 3825-3830.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.3 pharmacology enzyme is a target for inhibitor design for construction of antimicrobial agents Saccharomyces cerevisiae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.3 10 mg/ml purified recombinant enzyme, in TAPS, pH 8.5, in complex with inhibitor 4,4'-thiobis[2-(1-methylethyl)-phenol] in a molar ratio of 1:1, precipitant solution contains either 0.1 CHES, pH 9.5, 35% PEG 600 or 0.1 M CHES, pH 8.5, 40% PEG 400, and 0.2 M NaCl, 0.005 ml protein complex solution are equilibrated against 0.7 ml of precipitant solution using sitting drop technique, 3 months, X-ray diffraction structure determination and analysis at 3.0 A resolution, modeling Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.3 2,2'-[thiobis[[2-(1,1-dimethylethyl)-5-methyl-4,1-phenylene]oxy]]bis-acetic acid diethyl ester
-
 Saccharomyces cerevisiae
1.1.1.3 4,4'-thiobis[2-(1,1-dimethylethyl)]-5-methyl-phenol
-
 Saccharomyces cerevisiae
1.1.1.3 4,4'-thiobis[2-(1,1-dimethylethyl)]-phenol
-
 Saccharomyces cerevisiae
1.1.1.3 4,4'-thiobis[2-(1-methylethyl)]-phenol
-
 Saccharomyces cerevisiae
1.1.1.3 4,4'-thiobis[5-methyl-2-(1-methylethyl)]-phenol
-
 Saccharomyces cerevisiae
1.1.1.3 4,4'-[1,2-ethanediylbis(thio)]bis[2,6-bis(1-methylpropyl)]-phenol
-
 Saccharomyces cerevisiae
1.1.1.3 4,4'-[1,2-ethanediylbis(thio)]bis[2-(1,1-dimethylethyl)-6-methyl]-phenol
-
 Saccharomyces cerevisiae
1.1.1.3 4-(1-methylheptyl)-1,3-benzenediol
-
 Saccharomyces cerevisiae
1.1.1.3 4-[[2-(2-furanyl)ethyl]thio]-phenol
-
 Saccharomyces cerevisiae
1.1.1.3 4-[[[4-(1,1-dimethylethyl)phenyl]thio]methyl]-2,6-bis(1-methylethyl)-phenol
-
 Saccharomyces cerevisiae
1.1.1.3 bis(4-chlorophenyl)ethyloxiranyl-silane
-
 Saccharomyces cerevisiae
1.1.1.3 additional information no inhibition by [2-(1,1-dimethylethyl)-4-[[5-(1,1-dimethylethyl)-4-hydroxy-2-methylphenyl]thio]-5-methylphenoxy]-acetic acid and 4-amino-butyric acid 2-tert-butyl-4-(3-tert-butyl-4-hydroxy-phenylsulfanyl)-phenyl ester Saccharomyces cerevisiae
1.1.1.3 [2-(1,1-dimethylethyl)-4-[[5-(1,1-dimethylethyl)-4-hydroxy-2-methylphenyl]thio]-5-methylphenoxy]-acetic acid ethyl ester
-
 Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.3 L-aspartate 4-semialdehyde + NAD(P)H Saccharomyces cerevisiae essential step in amino acids L-methionine, L-threonine, and L-isoleucine biosynthesis L-homoserine + NAD(P)+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.3 Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.3 L-aspartate 4-semialdehyde + NAD(P)H
-
 Saccharomyces cerevisiae L-homoserine + NAD(P)+
-
 ?
1.1.1.3 L-aspartate 4-semialdehyde + NAD(P)H essential step in amino acids L-methionine, L-threonine, and L-isoleucine biosynthesis Saccharomyces cerevisiae L-homoserine + NAD(P)+
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.3 NADH
-
 Saccharomyces cerevisiae
1.1.1.3 NADPH
-
 Saccharomyces cerevisiae