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Literature summary extracted from

  • Laurinavicius, V.; Razumiene, J.; Kurtinaitiene, B.; Gureviciene, V.; Marcinkeviciene, L.; Bachmatova, I.
    Comparative characterization of soluble and membrane-bound PQQ-glucose dehydrogenases (2003), Biologia (Bratisl. ), 2, 31-34.
No PubMed abstract available

Application

EC Number Application Comment Organism
1.1.5.2 diagnostics purified enzyme is immobilized on carbon electrodes modified with 4-ferrocenylphenol, 4-(4-ferrocenylimino-methyl)-phenol, or 4-ferrocenylnitrophenol, for use as glucose biosensors, kinetic behaviour during immobilization Erwinia sp.
1.1.5.2 diagnostics purified enzyme is immobilized on carbon electrodes modified with 4-ferrocenylphenol, 4-(4-ferrocenylimino-methyl)phenol, or 4-ferrocenylnitrophenol, for use as glucose biosensors, kinetic behaviour during immobilization Acinetobacter calcoaceticus

General Stability

EC Number General Stability Organism
1.1.5.2 the membrane-bound enzyme is more stable than the soluble form Erwinia sp.
1.1.5.2 the soluble enzyme is less stable than the membrane-bound form Acinetobacter calcoaceticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.5.2 0.73
-
 4-(4-ferrocenylimino-methyl)phenol s-GDH Acinetobacter calcoaceticus
1.1.5.2 0.83
-
 4-ferrocenylphenol m-GDH Erwinia sp.
1.1.5.2 0.89
-
 4-ferrocenylnitrophenol s-GDH Acinetobacter calcoaceticus
1.1.5.2 1
-
 4-ferrocenylphenol s-GDH Acinetobacter calcoaceticus
1.1.5.2 2.43
-
 4-(4-ferrocenylimino-methyl)phenol m-GDH Erwinia sp.
1.1.5.2 2.73
-
 4-ferrocenylnitrophenol m-GDH Erwinia sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.5.2 membrane bound enzyme m-GDH Erwinia sp. 16020
-
1.1.5.2 soluble enzyme s-GDH Acinetobacter calcoaceticus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.5.2 Ca2+ required Acinetobacter calcoaceticus
1.1.5.2 Ca2+ required Erwinia sp.

Organism

EC Number Organism UniProt Comment Textmining
1.1.5.2 Acinetobacter calcoaceticus
-
 enzyme s-GDH
-
1.1.5.2 Acinetobacter calcoaceticus L.M.D. 79.41
-
 enzyme s-GDH
-
1.1.5.2 Erwinia sp.
-
 m-GDH
-
1.1.5.2 Erwinia sp. 34-1
-
 m-GDH
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.5.2 12
-
 purified native m-GDH Erwinia sp.
1.1.5.2 250
-
 purified native s-GDH Acinetobacter calcoaceticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.5.2 cellobiose + ubiquinone
-
 Acinetobacter calcoaceticus ? + ubiquinol
-
 ?
1.1.5.2 cellobiose + ubiquinone
-
 Acinetobacter calcoaceticus L.M.D. 79.41 ? + ubiquinol
-
 ?
1.1.5.2 D-galactose + ubiquinone
-
 Acinetobacter calcoaceticus D-galactono-1,5-lactone + ubiquinol
-
 ?
1.1.5.2 D-galactose + ubiquinone
-
 Erwinia sp. D-galactono-1,5-lactone + ubiquinol
-
 ?
1.1.5.2 D-galactose + ubiquinone
-
 Erwinia sp. 34-1 D-galactono-1,5-lactone + ubiquinol
-
 ?
1.1.5.2 D-galactose + ubiquinone
-
 Acinetobacter calcoaceticus L.M.D. 79.41 D-galactono-1,5-lactone + ubiquinol
-
 ?
1.1.5.2 D-glucose + 4-(4-ferrocenylimino-methyl)phenol
-
 Acinetobacter calcoaceticus D-glucono-1,5-lactone + ?
-
 ?
1.1.5.2 D-glucose + 4-(4-ferrocenylimino-methyl)phenol
-
 Erwinia sp. D-glucono-1,5-lactone + ?
-
 r
1.1.5.2 D-glucose + 4-(4-ferrocenylimino-methyl)phenol
-
 Erwinia sp. 34-1 D-glucono-1,5-lactone + ?
-
 r
1.1.5.2 D-glucose + 4-(4-ferrocenylimino-methyl)phenol
-
 Acinetobacter calcoaceticus L.M.D. 79.41 D-glucono-1,5-lactone + ?
-
 ?
1.1.5.2 D-glucose + 4-ferrocenylnitrophenol
-
 Acinetobacter calcoaceticus D-glucono-1,5-lactone + ?
-
 ?
1.1.5.2 D-glucose + 4-ferrocenylnitrophenol
-
 Erwinia sp. D-glucono-1,5-lactone + ?
-
 ?
1.1.5.2 D-glucose + 4-ferrocenylnitrophenol
-
 Erwinia sp. 34-1 D-glucono-1,5-lactone + ?
-
 ?
1.1.5.2 D-glucose + 4-ferrocenylphenol
-
 Acinetobacter calcoaceticus D-glucono-1,5-lactone + ?
-
 ?
1.1.5.2 D-glucose + 4-ferrocenylphenol
-
 Erwinia sp. D-glucono-1,5-lactone + ?
-
 ?
1.1.5.2 D-glucose + ubiquinone best substrate Acinetobacter calcoaceticus D-glucono-1,5-lactone + ubiquinol
-
 ?
1.1.5.2 D-glucose + ubiquinone best substrate Erwinia sp. D-glucono-1,5-lactone + ubiquinol
-
 ?
1.1.5.2 D-glucose + ubiquinone best substrate Erwinia sp. 34-1 D-glucono-1,5-lactone + ubiquinol
-
 ?
1.1.5.2 D-glucose + ubiquinone best substrate Acinetobacter calcoaceticus L.M.D. 79.41 D-glucono-1,5-lactone + ubiquinol
-
 ?
1.1.5.2 L-arabinose + ubiquinone
-
 Acinetobacter calcoaceticus L-arabino-1,5-lactone + ubiquinol
-
 ?
1.1.5.2 L-arabinose + ubiquinone
-
 Erwinia sp. L-arabino-1,5-lactone + ubiquinol
-
 ?
1.1.5.2 lactose + ubiquinone
-
 Acinetobacter calcoaceticus ? + ubiquinol
-
 ?
1.1.5.2 maltose + ubiquinone
-
 Acinetobacter calcoaceticus ? + ubiquinol
-
 ?
1.1.5.2 additional information substrate specificity of native and immobilized enzyme Acinetobacter calcoaceticus ?
-
 ?
1.1.5.2 additional information substrate specificity of native and immobilized enzyme, maltose is a poor substrate Erwinia sp. ?
-
 ?
1.1.5.2 additional information substrate specificity of native and immobilized enzyme, maltose is a poor substrate Erwinia sp. 34-1 ?
-
 ?
1.1.5.2 additional information substrate specificity of native and immobilized enzyme Acinetobacter calcoaceticus L.M.D. 79.41 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.5.2 m-GDH
-
 Erwinia sp.
1.1.5.2 PQQ-GDH
-
 Acinetobacter calcoaceticus
1.1.5.2 PQQ-GDH
-
 Erwinia sp.
1.1.5.2 PQQ-glucose dehydrogenase
-
 Acinetobacter calcoaceticus
1.1.5.2 PQQ-glucose dehydrogenase
-
 Erwinia sp.
1.1.5.2 s-GDH
-
 Acinetobacter calcoaceticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.5.2 additional information
-
 pH-dependence of native and immobilized enzyme Acinetobacter calcoaceticus
1.1.5.2 additional information
-
 pH-dependence of native and immobilized enzyme Erwinia sp.
1.1.5.2 5.5
-
 native m-GDH Erwinia sp.
1.1.5.2 6 9 immobilized s-GDH, broad maximum Acinetobacter calcoaceticus
1.1.5.2 6
-
 immobilized m-GDH Erwinia sp.
1.1.5.2 7
-
 native s-GDH Acinetobacter calcoaceticus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.5.2 pyrroloquinoline quinone i.e. PQQ, dependent on Acinetobacter calcoaceticus
1.1.5.2 pyrroloquinoline quinone i.e. PQQ, dependent on Erwinia sp.