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Literature summary extracted from

  • Andrews, A.J.; Hall, T.A.; Brown, J.W.
    Characterization of RNase P holoenzymes from Methanococcus jannaschii and Methanothermobacter thermoautotrophicus (2001), Biol. Chem., 382, 1171-1177.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.26.5 Ca2+ irreversible inactivation Methanocaldococcus jannaschii
3.1.26.5 Ca2+ irreversible inactivation Methanothermobacter thermautotrophicus
3.1.26.5 EDTA irreversible inactivation Methanocaldococcus jannaschii
3.1.26.5 EDTA irreversible inactivation Methanothermobacter thermautotrophicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.26.5 0.0000345
-
tRNA precursor pH 8.0, 65°C, in presence of 800 mM ammonium acetate and 5 mM MgCl2 Methanocaldococcus jannaschii
3.1.26.5 0.0000684
-
tRNA precursor pH 8.0, 50°C, in presence of 50 mM ammonium acetate and 30 mM MgCl2 Methanothermobacter thermautotrophicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.26.5 Mg2+ dependent on, at least half-maximal activity between 8-80 mM Methanothermobacter thermautotrophicus
3.1.26.5 Mg2+ dependent on, optimal at 2-10 mM Methanocaldococcus jannaschii
3.1.26.5 additional information at least half-maximal activity at 1 M ammonium acetate Methanothermobacter thermautotrophicus
3.1.26.5 additional information optimal ionic strength at 800 mM ammonium acetate at 60°C Methanocaldococcus jannaschii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.26.5 45000
-
about, gel filtration Methanocaldococcus jannaschii
3.1.26.5 400000
-
gel filtration Methanothermobacter thermautotrophicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.26.5 additional information Methanothermobacter thermautotrophicus phylogenetic study of archeal enzymes ?
-
?
3.1.26.5 additional information Methanocaldococcus jannaschii phylogenetic study of archeal enzymes ?
-
?
3.1.26.5 tRNA precursor + H2O Methanothermobacter thermautotrophicus cleavage of 5'-terminal oligonucleotide, maturation mature tRNA + 5'-oligonucleotide
-
?
3.1.26.5 tRNA precursor + H2O Methanocaldococcus jannaschii cleavage of 5'-terminal oligonucleotide, maturation mature tRNA + 5'-oligonucleotide
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.26.5 Methanocaldococcus jannaschii
-
type M enzyme
-
3.1.26.5 Methanothermobacter thermautotrophicus
-
formerly Methanobacterium thermoautotrophicum strain DELTAH, type A enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.26.5 partially Methanothermobacter thermautotrophicus
3.1.26.5 partially, 2400fold Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.26.5 additional information phylogenetic study of archeal enzymes Methanothermobacter thermautotrophicus ?
-
?
3.1.26.5 additional information phylogenetic study of archeal enzymes Methanocaldococcus jannaschii ?
-
?
3.1.26.5 tRNA precursor + H2O cleavage of 5'-terminal oligonucleotide, maturation Methanothermobacter thermautotrophicus mature tRNA + 5'-oligonucleotide
-
?
3.1.26.5 tRNA precursor + H2O cleavage of 5'-terminal oligonucleotide, maturation Methanocaldococcus jannaschii mature tRNA + 5'-oligonucleotide
-
?

Subunits

EC Number Subunits Comment Organism
3.1.26.5 More subunit composition, secondary structure of the enzyme RNA moiety Methanothermobacter thermautotrophicus
3.1.26.5 More subunit composition, secondary structure of the enzyme RNA moiety Methanocaldococcus jannaschii

Synonyms

EC Number Synonyms Comment Organism
3.1.26.5 RNase P
-
Methanothermobacter thermautotrophicus
3.1.26.5 RNase P
-
Methanocaldococcus jannaschii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.26.5 80
-
about Methanothermobacter thermautotrophicus
3.1.26.5 80
-
about Methanocaldococcus jannaschii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.26.5 70 80 and above, 50% activity at about 70°C Methanocaldococcus jannaschii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.26.5 0.57
-
tRNA precursor pH 8.0, 50°C, in presence of 50 mM ammonium acetate and 30 mM MgCl2 Methanothermobacter thermautotrophicus
3.1.26.5 0.88
-
tRNA precursor pH 8.0, 65°C, in presence of 800 mM ammonium acetate and 5 mM MgCl2 Methanocaldococcus jannaschii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.26.5 8
-
assay at Methanothermobacter thermautotrophicus
3.1.26.5 8
-
assay at Methanocaldococcus jannaschii