EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.26.5 | Ca2+ | irreversible inactivation | Methanocaldococcus jannaschii | |
3.1.26.5 | Ca2+ | irreversible inactivation | Methanothermobacter thermautotrophicus | |
3.1.26.5 | EDTA | irreversible inactivation | Methanocaldococcus jannaschii | |
3.1.26.5 | EDTA | irreversible inactivation | Methanothermobacter thermautotrophicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.26.5 | 0.0000345 | - |
tRNA precursor | pH 8.0, 65°C, in presence of 800 mM ammonium acetate and 5 mM MgCl2 | Methanocaldococcus jannaschii | |
3.1.26.5 | 0.0000684 | - |
tRNA precursor | pH 8.0, 50°C, in presence of 50 mM ammonium acetate and 30 mM MgCl2 | Methanothermobacter thermautotrophicus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.26.5 | Mg2+ | dependent on, at least half-maximal activity between 8-80 mM | Methanothermobacter thermautotrophicus | |
3.1.26.5 | Mg2+ | dependent on, optimal at 2-10 mM | Methanocaldococcus jannaschii | |
3.1.26.5 | additional information | at least half-maximal activity at 1 M ammonium acetate | Methanothermobacter thermautotrophicus | |
3.1.26.5 | additional information | optimal ionic strength at 800 mM ammonium acetate at 60°C | Methanocaldococcus jannaschii |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.26.5 | 45000 | - |
about, gel filtration | Methanocaldococcus jannaschii |
3.1.26.5 | 400000 | - |
gel filtration | Methanothermobacter thermautotrophicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.26.5 | additional information | Methanothermobacter thermautotrophicus | phylogenetic study of archeal enzymes | ? | - |
? | |
3.1.26.5 | additional information | Methanocaldococcus jannaschii | phylogenetic study of archeal enzymes | ? | - |
? | |
3.1.26.5 | tRNA precursor + H2O | Methanothermobacter thermautotrophicus | cleavage of 5'-terminal oligonucleotide, maturation | mature tRNA + 5'-oligonucleotide | - |
? | |
3.1.26.5 | tRNA precursor + H2O | Methanocaldococcus jannaschii | cleavage of 5'-terminal oligonucleotide, maturation | mature tRNA + 5'-oligonucleotide | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.26.5 | Methanocaldococcus jannaschii | - |
type M enzyme | - |
3.1.26.5 | Methanothermobacter thermautotrophicus | - |
formerly Methanobacterium thermoautotrophicum strain DELTAH, type A enzyme | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.26.5 | partially | Methanothermobacter thermautotrophicus |
3.1.26.5 | partially, 2400fold | Methanocaldococcus jannaschii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.26.5 | additional information | phylogenetic study of archeal enzymes | Methanothermobacter thermautotrophicus | ? | - |
? | |
3.1.26.5 | additional information | phylogenetic study of archeal enzymes | Methanocaldococcus jannaschii | ? | - |
? | |
3.1.26.5 | tRNA precursor + H2O | cleavage of 5'-terminal oligonucleotide, maturation | Methanothermobacter thermautotrophicus | mature tRNA + 5'-oligonucleotide | - |
? | |
3.1.26.5 | tRNA precursor + H2O | cleavage of 5'-terminal oligonucleotide, maturation | Methanocaldococcus jannaschii | mature tRNA + 5'-oligonucleotide | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.26.5 | More | subunit composition, secondary structure of the enzyme RNA moiety | Methanothermobacter thermautotrophicus |
3.1.26.5 | More | subunit composition, secondary structure of the enzyme RNA moiety | Methanocaldococcus jannaschii |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.26.5 | RNase P | - |
Methanothermobacter thermautotrophicus |
3.1.26.5 | RNase P | - |
Methanocaldococcus jannaschii |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.26.5 | 80 | - |
about | Methanothermobacter thermautotrophicus |
3.1.26.5 | 80 | - |
about | Methanocaldococcus jannaschii |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.26.5 | 70 | 80 | and above, 50% activity at about 70°C | Methanocaldococcus jannaschii |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.26.5 | 0.57 | - |
tRNA precursor | pH 8.0, 50°C, in presence of 50 mM ammonium acetate and 30 mM MgCl2 | Methanothermobacter thermautotrophicus | |
3.1.26.5 | 0.88 | - |
tRNA precursor | pH 8.0, 65°C, in presence of 800 mM ammonium acetate and 5 mM MgCl2 | Methanocaldococcus jannaschii |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.26.5 | 8 | - |
assay at | Methanothermobacter thermautotrophicus |
3.1.26.5 | 8 | - |
assay at | Methanocaldococcus jannaschii |