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Literature summary extracted from

  • Nasser, I.; Mohsen, A.W.; Jelesarov, I.; Vockley, J.; Macheroux, P.; Ghisla, S.
    Thermal unfolding of medium-chain acyl-CoA dehydrogenase and iso(3)valeryl-CoA dehydrogenase: study of the effect of genetic defects on enzyme stability (2004), Biochim. Biophys. Acta, 1690, 22-32.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.8.4 expression of wild-type and mutant enzyme in Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.3.8.4 A282V site-directed mutagenesis, severely affected interaction between enzyme and flavin cofactor, about 40% reduced activity compared to the wild-type enzyme Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.8.4 2-aza-isovaleryl-CoA substrate analogue Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.8.4 isovaleryl-CoA + electron transfer protein Homo sapiens
-
 3-methylcrotonyl-CoA + reduced electron transfer protein
-
 ?
1.3.8.4 additional information Homo sapiens physiological implications of enzyme mutations and deficiency ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.8.4 Homo sapiens
-
-
-
1.3.8.7 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.8.4 isovaleryl-CoA + electron transfer protein
-
 Homo sapiens 3-methylcrotonyl-CoA + reduced electron transfer protein
-
 ?
1.3.8.4 additional information physiological implications of enzyme mutations and deficiency Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.8.4 i3VD
-
 Homo sapiens
1.3.8.4 iso(3)valeryl-CoA dehydrogenase
-
 Homo sapiens
1.3.8.7 MCAD
-
 Homo sapiens
1.3.8.7 MCADH
-
 Homo sapiens
1.3.8.7 medium-chain acyl-CoA dehydrogenase
-
 Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.3.8.4 additional information
-
 thermal unfolding analysis Homo sapiens
1.3.8.4 48.2
-
 melting point of mutant A282V without bound ligand Homo sapiens
1.3.8.4 48.5
-
 melting point of mutant A282V with isovaleryl-CoA bound Homo sapiens
1.3.8.4 48.6
-
 melting point of mutant A282V with 2-aza-isovaleryl-CoA bound Homo sapiens
1.3.8.4 50.5
-
 melting point of wild-type enzyme without bound ligand Homo sapiens
1.3.8.4 50.9
-
 melting point of wild-type enzyme with 2-aza-isovaleryl-CoA bound Homo sapiens
1.3.8.4 55.4
-
 melting point of wild-type enzyme with isovaleryl-CoA bound Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.8.4 7.5
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.8.4 flavin
-
 Homo sapiens