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Literature summary extracted from

  • Dayan, F.E.; Kuhajek, J.M.; Canel, C.; Watson, S.B.; Moraes, R.M.
    Podophyllum peltatum possesses a beta-glucosidase with high substrate specificity for the aryltetralin lignan podophyllotoxin (2003), Biochim. Biophys. Acta, 1646, 157-163.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 AgNO3 0.5 mM, 81% inhibition, reversible by the addition of 50 mM beta-mercaptoethanol Podophyllum peltatum
3.2.1.21 HgCl2 0.5 mM, 77% inhibition, reversible by the addition of 50 mM beta-mercaptoethanol Podophyllum peltatum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.21 podophyllotoxin-4-O-beta-D-glucopyranoside + H2O Podophyllum peltatum
-
 podophyllotoxin + beta-D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Podophyllum peltatum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 quartenary amine column, Phenyl Sepharose Podophyllum peltatum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.21 leaf
-
 Podophyllum peltatum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.21 75800
-
-
 Podophyllum peltatum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 epipodophyllotoxin-4-O-beta-D-glucopyranoside + H2O 85% of activity with podophyllotoxin-4-O-beta-D-glucopyranoside Podophyllum peltatum epipodophyllotoxin + beta-D-glucose
-
 ?
3.2.1.21 laminaribiose + H2O very low activity with cellobiose, cellotriose, cellotetraose, cellopentaose and gentibiose Podophyllum peltatum beta-D-glucose + ?
-
 ?
3.2.1.21 podophyllotoxin-4-O-beta-D-glucopyranoside + H2O
-
 Podophyllum peltatum podophyllotoxin + beta-D-glucose
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 45
-
-
 Podophyllum peltatum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.21 10 60 approx. 50% of maximal activity at 30°C, approx. 10% of maximal activity at 60°C Podophyllum peltatum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.21
-
-
 approx. 20% loss of activity after 120 min Podophyllum peltatum
3.2.1.21 45
-
 50% loss of activity after 80 min Podophyllum peltatum
3.2.1.21 55
-
 50% loss of activity after 15 min Podophyllum peltatum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 5
-
-
 Podophyllum peltatum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.21 3 9 approx. 50% of maximal activity at pH 4.0 and pH 6.0, respectively Podophyllum peltatum