BRENDA - Enzyme Database show

Coupling between cyclooxygenases and prostaglandin F(2alpha) synthase. Detection of an inducible, glutathione-activated, membrane-bound prostaglandin F2alpha-synthetic activity

Nakashima, K.; Ueno, N.; Kamei, D.; Tanioka, T.; Nakatani, Y.; Murakami, M.; Kudo, I.; Biochim. Biophys. Acta 1633, 96-105 (2003)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.1.1.188
GSH
-
Homo sapiens
1.1.1.188
interleukin-1beta
membrane-associated, not soluble, isozyme in WI-38 cells
Homo sapiens
1.1.1.188
lipopolysaccharide
induces the membrane-associated isozyme
Rattus norvegicus
1.1.1.188
lipopolysaccharides
strong induction of the membrane-bound lung isozyme, slight induction in spleen, kidney, testis, and pancreas, overview
Mus musculus
1.1.1.188
additional information
PGF synthase I activity in HEK 293 cells is unaffected by interleukin-1beta
Homo sapiens
1.1.1.189
lipopolysaccharides
strong induction of the membrane-bound lung isozyme, significant induction in stomach, intestine, spleen, kidney, testis, and pancreas, overview
Mus musculus
Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.1.1.188
additional information
coexpression of COX-1 and COX-2 increases the endogenous cytosolic PGFS-I activity, but only slightly the membrane-bound activity, overview
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.188
FeCl2
-
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.188
cytosol
not-inducible isozyme PGFS-I
Homo sapiens
5829
-
1.1.1.188
cytosol
not-inducible isozyme
Rattus norvegicus
5829
-
1.1.1.188
membrane
inducible isozyme m-PGFS
Homo sapiens
16020
-
1.1.1.188
membrane
-
Mus musculus
16020
-
1.1.1.188
membrane
inducible isozyme m-PGFS
Rattus norvegicus
16020
-
1.1.1.189
membrane
-
Mus musculus
16020
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.188
additional information
Rattus norvegicus
enzyme interacts with cyclooxygenase 1 and 2, isozyme PGFS-I interacts predominantly with cyclooxygenase 2, i.e. COX-2
?
-
-
-
1.1.1.188
additional information
Homo sapiens
the cytosolic enzyme synergistically interacts with cyclooxygenase 1 and 2, coupling, isozyme PGFS-I interacts predominantly with cyclooxygenase 2, i.e. COX-2
?
-
-
-
1.1.1.188
prostaglandin H2 + NADPH
Mus musculus
-
prostaglandin F2alpha + NADP+
-
-
?
1.1.1.188
prostaglandin H2 + NADPH
Homo sapiens
-
prostaglandin F2alpha + NADP+
-
-
?
1.1.1.188
prostaglandin H2 + NADPH
Rattus norvegicus
-
prostaglandin F2alpha + NADP+
-
-
?
1.1.1.189
prostaglandin H2 + NADPH
Mus musculus
-
prostaglandin F2alpha + NADP+
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.188
Homo sapiens
-
2 isozymes PGFS-I and PGFS-II, soluble and membrane-associated isozymes
-
1.1.1.188
Mus musculus
-
-
-
1.1.1.188
Rattus norvegicus
-
isozymes: 1 soluble not-inducible and 1 membraneous inducible by lipopolysaccharides
-
1.1.1.189
Mus musculus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.188
brain
-
Mus musculus
-
1.1.1.188
GOTO cell
glioblastoma cell
Homo sapiens
-
1.1.1.188
heart
-
Mus musculus
-
1.1.1.188
HEK-293 cell
embryonic kidney cell, isozyme PGFS-I
Homo sapiens
-
1.1.1.188
HeLa cell
-
Homo sapiens
-
1.1.1.188
intestine
-
Mus musculus
-
1.1.1.188
kidney
-
Mus musculus
-
1.1.1.188
liver
-
Mus musculus
-
1.1.1.188
lung
-
Mus musculus
-
1.1.1.188
macrophage
peritoneal
Rattus norvegicus
-
1.1.1.188
pancreas
-
Mus musculus
-
1.1.1.188
spleen
-
Mus musculus
-
1.1.1.188
stomach
-
Mus musculus
-
1.1.1.188
testis
-
Mus musculus
-
1.1.1.188
thymus
-
Mus musculus
-
1.1.1.188
U-251 cell
glioblastoma cell
Homo sapiens
-
1.1.1.188
WI-38 cell
lung fibroblast cell
Homo sapiens
-
1.1.1.189
brain
-
Mus musculus
-
1.1.1.189
heart
-
Mus musculus
-
1.1.1.189
intestine
-
Mus musculus
-
1.1.1.189
kidney
-
Mus musculus
-
1.1.1.189
liver
-
Mus musculus
-
1.1.1.189
lung
-
Mus musculus
-
1.1.1.189
pancreas
-
Mus musculus
-
1.1.1.189
spleen
-
Mus musculus
-
1.1.1.189
stomach
-
Mus musculus
-
1.1.1.189
testis
-
Mus musculus
-
1.1.1.189
thymus
-
Mus musculus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.188
additional information
enzyme interacts with cyclooxygenase 1 and 2, isozyme PGFS-I interacts predominantly with cyclooxygenase 2, i.e. COX-2
654851
Rattus norvegicus
?
-
-
-
-
1.1.1.188
additional information
the cytosolic enzyme synergistically interacts with cyclooxygenase 1 and 2, coupling, isozyme PGFS-I interacts predominantly with cyclooxygenase 2, i.e. COX-2
654851
Homo sapiens
?
-
-
-
-
1.1.1.188
prostaglandin H2 + NADPH
-
654851
Mus musculus
prostaglandin F2alpha + NADP+
-
-
-
?
1.1.1.188
prostaglandin H2 + NADPH
-
654851
Homo sapiens
prostaglandin F2alpha + NADP+
-
-
-
?
1.1.1.188
prostaglandin H2 + NADPH
-
654851
Rattus norvegicus
prostaglandin F2alpha + NADP+
-
-
-
?
1.1.1.189
prostaglandin H2 + NADPH
-
654851
Mus musculus
prostaglandin F2alpha + NADP+
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.188
24
-
assay at
Homo sapiens
1.1.1.188
24
-
assay at
Rattus norvegicus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.188
8
-
assay at
Homo sapiens
1.1.1.188
8
-
assay at
Rattus norvegicus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.188
NADPH
-
Rattus norvegicus
1.1.1.188
NADPH
-
Homo sapiens
1.1.1.188
NADPH
-
Mus musculus
1.1.1.189
NADPH
-
Mus musculus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.1.1.188
GSH
-
Homo sapiens
1.1.1.188
interleukin-1beta
membrane-associated, not soluble, isozyme in WI-38 cells
Homo sapiens
1.1.1.188
lipopolysaccharide
induces the membrane-associated isozyme
Rattus norvegicus
1.1.1.188
lipopolysaccharides
strong induction of the membrane-bound lung isozyme, slight induction in spleen, kidney, testis, and pancreas, overview
Mus musculus
1.1.1.188
additional information
PGF synthase I activity in HEK 293 cells is unaffected by interleukin-1beta
Homo sapiens
1.1.1.189
lipopolysaccharides
strong induction of the membrane-bound lung isozyme, significant induction in stomach, intestine, spleen, kidney, testis, and pancreas, overview
Mus musculus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.188
NADPH
-
Rattus norvegicus
1.1.1.188
NADPH
-
Homo sapiens
1.1.1.188
NADPH
-
Mus musculus
1.1.1.189
NADPH
-
Mus musculus
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.1.1.188
additional information
coexpression of COX-1 and COX-2 increases the endogenous cytosolic PGFS-I activity, but only slightly the membrane-bound activity, overview
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.188
FeCl2
-
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.188
cytosol
not-inducible isozyme PGFS-I
Homo sapiens
5829
-
1.1.1.188
cytosol
not-inducible isozyme
Rattus norvegicus
5829
-
1.1.1.188
membrane
inducible isozyme m-PGFS
Homo sapiens
16020
-
1.1.1.188
membrane
-
Mus musculus
16020
-
1.1.1.188
membrane
inducible isozyme m-PGFS
Rattus norvegicus
16020
-
1.1.1.189
membrane
-
Mus musculus
16020
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.188
additional information
Rattus norvegicus
enzyme interacts with cyclooxygenase 1 and 2, isozyme PGFS-I interacts predominantly with cyclooxygenase 2, i.e. COX-2
?
-
-
-
1.1.1.188
additional information
Homo sapiens
the cytosolic enzyme synergistically interacts with cyclooxygenase 1 and 2, coupling, isozyme PGFS-I interacts predominantly with cyclooxygenase 2, i.e. COX-2
?
-
-
-
1.1.1.188
prostaglandin H2 + NADPH
Mus musculus
-
prostaglandin F2alpha + NADP+
-
-
?
1.1.1.188
prostaglandin H2 + NADPH
Homo sapiens
-
prostaglandin F2alpha + NADP+
-
-
?
1.1.1.188
prostaglandin H2 + NADPH
Rattus norvegicus
-
prostaglandin F2alpha + NADP+
-
-
?
1.1.1.189
prostaglandin H2 + NADPH
Mus musculus
-
prostaglandin F2alpha + NADP+
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.188
brain
-
Mus musculus
-
1.1.1.188
GOTO cell
glioblastoma cell
Homo sapiens
-
1.1.1.188
heart
-
Mus musculus
-
1.1.1.188
HEK-293 cell
embryonic kidney cell, isozyme PGFS-I
Homo sapiens
-
1.1.1.188
HeLa cell
-
Homo sapiens
-
1.1.1.188
intestine
-
Mus musculus
-
1.1.1.188
kidney
-
Mus musculus
-
1.1.1.188
liver
-
Mus musculus
-
1.1.1.188
lung
-
Mus musculus
-
1.1.1.188
macrophage
peritoneal
Rattus norvegicus
-
1.1.1.188
pancreas
-
Mus musculus
-
1.1.1.188
spleen
-
Mus musculus
-
1.1.1.188
stomach
-
Mus musculus
-
1.1.1.188
testis
-
Mus musculus
-
1.1.1.188
thymus
-
Mus musculus
-
1.1.1.188
U-251 cell
glioblastoma cell
Homo sapiens
-
1.1.1.188
WI-38 cell
lung fibroblast cell
Homo sapiens
-
1.1.1.189
brain
-
Mus musculus
-
1.1.1.189
heart
-
Mus musculus
-
1.1.1.189
intestine
-
Mus musculus
-
1.1.1.189
kidney
-
Mus musculus
-
1.1.1.189
liver
-
Mus musculus
-
1.1.1.189
lung
-
Mus musculus
-
1.1.1.189
pancreas
-
Mus musculus
-
1.1.1.189
spleen
-
Mus musculus
-
1.1.1.189
stomach
-
Mus musculus
-
1.1.1.189
testis
-
Mus musculus
-
1.1.1.189
thymus
-
Mus musculus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.188
additional information
enzyme interacts with cyclooxygenase 1 and 2, isozyme PGFS-I interacts predominantly with cyclooxygenase 2, i.e. COX-2
654851
Rattus norvegicus
?
-
-
-
-
1.1.1.188
additional information
the cytosolic enzyme synergistically interacts with cyclooxygenase 1 and 2, coupling, isozyme PGFS-I interacts predominantly with cyclooxygenase 2, i.e. COX-2
654851
Homo sapiens
?
-
-
-
-
1.1.1.188
prostaglandin H2 + NADPH
-
654851
Mus musculus
prostaglandin F2alpha + NADP+
-
-
-
?
1.1.1.188
prostaglandin H2 + NADPH
-
654851
Homo sapiens
prostaglandin F2alpha + NADP+
-
-
-
?
1.1.1.188
prostaglandin H2 + NADPH
-
654851
Rattus norvegicus
prostaglandin F2alpha + NADP+
-
-
-
?
1.1.1.189
prostaglandin H2 + NADPH
-
654851
Mus musculus
prostaglandin F2alpha + NADP+
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.188
24
-
assay at
Homo sapiens
1.1.1.188
24
-
assay at
Rattus norvegicus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.188
8
-
assay at
Homo sapiens
1.1.1.188
8
-
assay at
Rattus norvegicus