EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.55 | Ag+ | 92% inhibition of AFQ1, 89% inhibition of AFS, at 1 mM | Penicillium chrysogenum | |
3.2.1.55 | Cd2+ | 37% inhibition of AFQ1, 15% inhibition of AFS, at 1 mM | Penicillium chrysogenum | |
3.2.1.55 | Cu2+ | 76% inhibition of AFQ1, 36% inhibition of AFS, at 1 mM | Penicillium chrysogenum | |
3.2.1.55 | Hg2+ | 90% inhibition of AFQ1, 74% inhibition of AFS, at 1 mM | Penicillium chrysogenum | |
3.2.1.55 | L-arabinose | 10 mg/ml, inhibition of enzyme AFS1, not AFQ1 | Penicillium chrysogenum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.55 | 0.86 | - |
4-nitrophenyl-alpha-L-arabinofuranoside | enzyme AFQ1, pH 5.0, 37°C | Penicillium chrysogenum | |
3.2.1.55 | 1.32 | - |
4-nitrophenyl-alpha-L-arabinofuranoside | enzyme AFS1, pH 5.0, 37°C | Penicillium chrysogenum |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.2.1.55 | extracellular | - |
Penicillium chrysogenum | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.55 | 46000 | - |
type B enzyme AFS1, gel filtration | Penicillium chrysogenum |
3.2.1.55 | 52000 | - |
x * 79000, type B enzyme AFQ1, x * 52000, type B enzyme AFS1 | Penicillium chrysogenum |
3.2.1.55 | 71000 | - |
type B enzyme AFQ1, gel filtration | Penicillium chrysogenum |
3.2.1.55 | 79000 | - |
x * 79000, type B enzyme AFQ1, x * 52000, type B enzyme AFS1 | Penicillium chrysogenum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.55 | Penicillium chrysogenum | - |
strain 31B, 2 distinct type B enzymes encoded by different genes | - |
3.2.1.55 | Penicillium chrysogenum 31B | - |
strain 31B, 2 distinct type B enzymes encoded by different genes | - |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.2.1.55 | glycoprotein | enzyme AFQ1 is a glycoprotein, while enzyme AFS1 is not | Penicillium chrysogenum |
3.2.1.55 | no glycoprotein | AFS1 | Penicillium chrysogenum |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.55 | both type B enzymes AFQ1, 17fold, and AFS1, 39.7fold, from culture filtrate | Penicillium chrysogenum |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.55 | 5.8 | - |
purified enzyme AFQ1 | Penicillium chrysogenum |
3.2.1.55 | 13.5 | - |
purified enzyme AFS1 | Penicillium chrysogenum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.55 | 4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Penicillium chrysogenum | 4-nitrophenol + alpha-L-arabinofuranose | - |
? | |
3.2.1.55 | 4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Penicillium chrysogenum 31B | 4-nitrophenol + alpha-L-arabinofuranose | - |
? | |
3.2.1.55 | 4-nitrophenyl beta-D-xylopyranoside + H2O | low activity only of enzyme AFS1, not AFQ1 | Penicillium chrysogenum | 4-nitrophenol + beta-D-xylopyranose | - |
? | |
3.2.1.55 | 4-nitrophenyl beta-D-xylopyranoside + H2O | low activity only of enzyme AFS1, not AFQ1 | Penicillium chrysogenum 31B | 4-nitrophenol + beta-D-xylopyranose | - |
? | |
3.2.1.55 | arabino-oligosaccharides + H2O | - |
Penicillium chrysogenum | alpha-L-arabinose | - |
? | |
3.2.1.55 | arabino-oligosaccharides + H2O | - |
Penicillium chrysogenum 31B | alpha-L-arabinose | - |
? | |
3.2.1.55 | arabinogalactan + H2O | from soybean | Penicillium chrysogenum | alpha-L-arabinose + ? | - |
? | |
3.2.1.55 | arabinogalactan + H2O | from soybean | Penicillium chrysogenum 31B | alpha-L-arabinose + ? | - |
? | |
3.2.1.55 | arabinoxylan + H2O | from wheat | Penicillium chrysogenum | alpha-L-arabinose + ? | - |
? | |
3.2.1.55 | branched L-arabinan + H2O | from sugar beet | Penicillium chrysogenum | L-arabinose + ? | - |
? | |
3.2.1.55 | debranched arabinan + H2O | from sugar beet | Penicillium chrysogenum | alpha-L-arabinose + ? | - |
? | |
3.2.1.55 | additional information | both type B enzymes show broad substrate specificity, synergistic action between AFQ1, AFS1, and exo-arabinanase in degradation of arabinan | Penicillium chrysogenum | ? | - |
? | |
3.2.1.55 | additional information | both type B enzymes show broad substrate specificity, synergistic action between AFQ1, AFS1, and exo-arabinanase in degradation of arabinan | Penicillium chrysogenum 31B | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.55 | ? | x * 79000, type B enzyme AFQ1, x * 52000, type B enzyme AFS1 | Penicillium chrysogenum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.55 | AF | - |
Penicillium chrysogenum |
3.2.1.55 | AFQ | - |
Penicillium chrysogenum |
3.2.1.55 | AFS | - |
Penicillium chrysogenum |
3.2.1.55 | alpha-L-arabinofuranosidase | - |
Penicillium chrysogenum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.55 | 50 | - |
- |
Penicillium chrysogenum |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.55 | 50 | - |
stable up to | Penicillium chrysogenum |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.55 | 14.3 | - |
4-nitrophenyl-alpha-L-arabinofuranoside | enzyme AFQ1, pH 5.0, 37°C | Penicillium chrysogenum | |
3.2.1.55 | 59.3 | - |
4-nitrophenyl-alpha-L-arabinofuranoside | enzyme AFS1, pH 5.0, 37°C | Penicillium chrysogenum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.55 | 3.3 | 5 | enzyme AFS1 | Penicillium chrysogenum |
3.2.1.55 | 4 | 6.5 | enzyme AFQ1 | Penicillium chrysogenum |