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Literature summary extracted from
- Purification and characterization of two exo-polygalacturonases from Aspergillus niger able to degrade xylogalacturonan and acetylated homogalacturonan (2002), Biochim. Biophys. Acta, 1572, 10-18.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.67 | HgCl2 | 0.01 mM, 3.4fold increase in activity, exo-PG2, no effect with exo-PG1 | Aspergillus niger |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.67 | 56000 | - |
x * 82000, exo-PG1, x * 56000, exo-PG2, SDS-PAGE | Aspergillus niger |
| 3.2.1.67 | 82000 | - |
x * 82000, exo-PG1, x * 56000, exo-PG2, SDS-PAGE | Aspergillus niger |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.67 | Aspergillus niger | - |
two isozymes | - |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.2.1.67 | glycoprotein | molecular mass after deglycosilation: 71000, exo-PG1, 43000, exo-PG2, deglycosylated proteins show about 50% residual activity | Aspergillus niger |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.67 | - |
Aspergillus niger |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.67 | 13.5 | - |
exo-PG1, pH 4.0, 40°C | Aspergillus niger |
| 3.2.1.67 | 31.4 | - |
exo-PG2, pH 4.0, 40°C | Aspergillus niger |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.67 | (1,4-alpha-D-galacturonide)n + H2O | - |
Aspergillus niger | D-galacturonic acid | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.67 | ? | x * 82000, exo-PG1, x * 56000, exo-PG2, SDS-PAGE | Aspergillus niger |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.67 | 60 | - |
- |
Aspergillus niger |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.67 | 60 | - |
30 min, 75% residual activity, exo-PG1, 25% residual activity, exo-PG2 | Aspergillus niger |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.67 | 3.4 | 3.8 | exo-PG1 | Aspergillus niger |
| 3.2.1.67 | 3.4 | 4.2 | exo-PG2 | Aspergillus niger |
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