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Literature summary extracted from

  • Sauer, J.; Sigurskjold, B.W.; Christensen, U.; Frandsen, T.P.; Mirgorodskaya, E.; Harrison, M.; Roepstorff, P.; Svensson, B.
    Glucoamylase: structure/function relationships, and protein engineering (2000), Biochim. Biophys. Acta, 1543, 275-293.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.3 expression in Pichia pastoris, Saccharomyces cerevisiae and Aspergillus niger Aspergillus awamori

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.3
-
 Saccharomycopsis fibuligera
3.2.1.3 crystal structure of glucoamylase at 2.2-2.4 A resolution Aspergillus awamori

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.3 E180Q mutant of G1, 48% of wild-type kcat for maltose, 88% of kcat for maltotriose Aspergillus niger
3.2.1.3 E400C further oxidation of Cys thiol group to sulfinic acid, up to 300% higher kcat and decreased Km compared to wild-type, depending on substrate Aspergillus awamori
3.2.1.3 W120F mutant of G1, 3% of wild-type kcat for maltose, 2% of kcat for maltotriose Aspergillus niger
3.2.1.3 W317F mutant of G1, 90% of wild-type kcat for maltose, 97% of kcat for maltotriose Aspergillus niger
3.2.1.3 W52F mutant of G2, almost no activity with maltose and maltotriose Aspergillus niger

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.3 1-deoxynojirimycin
-
 Aspergillus awamori
3.2.1.3 acarbose
-
 Aspergillus awamori
3.2.1.3 beta-O-acarviosine
-
 Aspergillus awamori

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.3 0.032
-
 maltotetraose 8°C, pH 4.5, wild-type G1 Aspergillus niger
3.2.1.3 0.058
-
 maltotetraose 8°C, pH 4.5, wild-type G2 Aspergillus niger
3.2.1.3 0.1
-
 maltopentaose recombinant glucoamylase Aspergillus awamori
3.2.1.3 0.11
-
 maltohexaose recombinant glucoamylase Aspergillus awamori
3.2.1.3 0.12
-
 maltoheptaose recombinant glucoamylase Aspergillus awamori
3.2.1.3 0.18
-
 maltose 8°C, pH 4.5, wild-type G1 Aspergillus niger
3.2.1.3 0.18
-
 maltotetraose recombinant glucoamylase Aspergillus awamori
3.2.1.3 0.28
-
 maltose 8°C, pH 4.5, wild-type G2 Aspergillus niger
3.2.1.3 0.29
-
 maltotriose recombinant glucoamylase Aspergillus awamori
3.2.1.3 3
-
 maltose recombinant glucoamylase Aspergillus awamori
3.2.1.3 15
-
 isomaltotetraose recombinant glucoamylase Aspergillus awamori
3.2.1.3 16
-
 isomaltopentaose recombinant glucoamylase Aspergillus awamori
3.2.1.3 17
-
 isomaltoheptaose recombinant glucoamylase Aspergillus awamori
3.2.1.3 18
-
 isomaltotriose recombinant glucoamylase Aspergillus awamori
3.2.1.3 18
-
 isomaltohexaose recombinant glucoamylase Aspergillus awamori
3.2.1.3 38
-
 isomaltose recombinant glucoamylase Aspergillus awamori

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.3 82327
-
 x * 82327, mass spectroscopy, recombinant GA expressed in Pichia pastoris Aspergillus awamori
3.2.1.3 82839
-
 x * 82839, mass spectroscopy, recombinant GA expressed in Aspergillus niger Aspergillus awamori
3.2.1.3 83869
-
 x * 83869, mass spectroscopy, recombinant GA expressed in Saccharomyces cerevisiae Aspergillus awamori

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.3 starch + H2O Aspergillus niger
-
 beta-D-glucose + ?
-
 ?
3.2.1.3 starch + H2O Aspergillus awamori
-
 beta-D-glucose + ?
-
 ?
3.2.1.3 starch + H2O Saccharomycopsis fibuligera
-
 beta-D-glucose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.3 Aspergillus awamori
-
-
-
3.2.1.3 Aspergillus niger
-
-
-
3.2.1.3 Saccharomycopsis fibuligera
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.3 glycoprotein
-
 Aspergillus awamori

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.3 isomaltoheptaose + H2O
-
 Aspergillus awamori beta-D-glucose
-
 ?
3.2.1.3 isomaltohexaose + H2O
-
 Aspergillus awamori beta-D-glucose
-
 ?
3.2.1.3 isomaltopentaose + H2O
-
 Aspergillus awamori beta-D-glucose
-
 ?
3.2.1.3 isomaltose + H2O
-
 Aspergillus awamori beta-D-glucose
-
 ?
3.2.1.3 isomaltotetraose + H2O
-
 Aspergillus awamori beta-D-glucose
-
 ?
3.2.1.3 isomaltotriose + H2O
-
 Aspergillus awamori beta-D-glucose
-
 ?
3.2.1.3 maltoheptaose + H2O
-
 Aspergillus awamori maltohexaose + D-glucose
-
 ?
3.2.1.3 maltohexaose + H2O
-
 Aspergillus awamori maltopentaose + D-glucose
-
 ?
3.2.1.3 maltopentaose + H2O
-
 Aspergillus awamori maltotetraose + beta-D-glucose
-
 ?
3.2.1.3 maltose + H2O
-
 Aspergillus niger beta-D-glucose + D-glucose
-
 ?
3.2.1.3 maltose + H2O
-
 Aspergillus awamori beta-D-glucose + D-glucose
-
 ?
3.2.1.3 maltotetraose + H2O
-
 Aspergillus niger ?
-
 ?
3.2.1.3 maltotetraose + H2O
-
 Aspergillus awamori maltotriose + beta-D-glucose
-
 ?
3.2.1.3 maltotriose + 2 H2O
-
 Aspergillus awamori 3 beta-D-glucose
-
 ?
3.2.1.3 starch + H2O
-
 Aspergillus niger beta-D-glucose + ?
-
 ?
3.2.1.3 starch + H2O
-
 Aspergillus awamori beta-D-glucose + ?
-
 ?
3.2.1.3 starch + H2O
-
 Saccharomycopsis fibuligera beta-D-glucose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.3 ? x * 82327, mass spectroscopy, recombinant GA expressed in Pichia pastoris Aspergillus awamori
3.2.1.3 ? x * 82839, mass spectroscopy, recombinant GA expressed in Aspergillus niger Aspergillus awamori
3.2.1.3 ? x * 83869, mass spectroscopy, recombinant GA expressed in Saccharomyces cerevisiae Aspergillus awamori

Synonyms

EC Number Synonyms Comment Organism
3.2.1.3 GA
-
 Aspergillus niger
3.2.1.3 GA
-
 Aspergillus awamori
3.2.1.3 GA
-
 Saccharomycopsis fibuligera

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.3 0.27
-
 isomaltose recombinant glucoamylase Aspergillus awamori
3.2.1.3 0.33
-
 maltose 8°C, pH 4.5, wild-type G1 Aspergillus niger
3.2.1.3 0.46
-
 maltose 8°C, pH 4.5, wild-type G2 Aspergillus niger
3.2.1.3 0.64
-
 isomaltotriose recombinant glucoamylase Aspergillus awamori
3.2.1.3 0.99
-
 isomaltotetraose recombinant glucoamylase Aspergillus awamori
3.2.1.3 1.2
-
 isomaltopentaose recombinant glucoamylase Aspergillus awamori
3.2.1.3 1.3
-
 isomaltoheptaose recombinant glucoamylase Aspergillus awamori
3.2.1.3 1.5
-
 isomaltohexaose recombinant glucoamylase Aspergillus awamori
3.2.1.3 2 8 maltotriose recombinant glucoamylase Aspergillus awamori
3.2.1.3 3.2
-
 maltotetraose 8°C, pH 4.5, wild-type G1 and G2 Aspergillus niger
3.2.1.3 6.8
-
 maltose recombinant glucoamylase Aspergillus awamori
3.2.1.3 44
-
 maltopentaose recombinant glucoamylase Aspergillus awamori
3.2.1.3 45
-
 maltotetraose recombinant glucoamylase Aspergillus awamori
3.2.1.3 49
-
 maltohexaose recombinant glucoamylase Aspergillus awamori
3.2.1.3 54
-
 maltoheptaose recombinant glucoamylase Aspergillus awamori

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.3 0.000000001
-
 acarbose
-
 Aspergillus awamori
3.2.1.3 0.000098
-
 beta-O-acarviosine
-
 Aspergillus awamori
3.2.1.3 0.046
-
 1-deoxynojirimycin
-
 Aspergillus awamori