BRENDA - Enzyme Database show

Crystal structure of human prostaglandin F synthase (AKR1C3)

Komoto, J.; Yamada, T.; Watanabe, K.; Takusagawa, F.; Biochemistry 43, 2188-2198 (2004)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.1.1.188
expression in Escherichia coli HB101
Homo sapiens
Crystallization (Commentary)
EC Number
Crystallization
Organism
1.1.1.188
20 mg/ml purified recombinant enzyme in a solution containing 1.4 mM PGD2 or inhibitor rutin, 1.2 mM NADP+ and NADPH, 50 mM MES, pH 6.0, and 25% w/v PEG 8000, hanging drop vapour diffusion method, 2-3 days, thick plate-shaped crystals of enzyme with NADP+ and PGD2 and of enzyme with NADPH and rutin, X-ray diffraction structure determination and analysis at 1.69 A resolution
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.188
rutin
binding structure at the substrate binding site
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.188
Homo sapiens
P42330
-
-
Reaction
EC Number
Reaction
Commentary
Organism
1.1.1.188
(5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP+ = (5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + NADPH + H+
substrate binding and catalytic mechanism, catalytic site residues are Y55 and H117, enzyme also shows 3alpha-hydroxysteroid dehydrogenase type II activity, i.e. 3alpha-HSD
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.188
lung
-
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.188
additional information
Y55 and H117 are involved in catalysis, enzyme also shows 3alpha-hydroxysteroid dehydrogenase type II activity
654740
Homo sapiens
?
-
-
-
-
1.1.1.188
prostaglandin D2 + NADPH
-
654740
Homo sapiens
9alpha,11beta-prostaglandin F2alpha + NADP+
-
-
-
r
1.1.1.188
prostaglandin H2 + NADPH
-
654740
Homo sapiens
prostaglandin F2alpha + NADP+
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.188
More
enzyme has a (alpha/beta)8 barrel structure, from crystal structure determination
Homo sapiens
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.188
NADP+
binding structure at the active site
Homo sapiens
1.1.1.188
NADPH
binding structure at the active site, catalytic structure and mechanism involving the cofactor
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.188
expression in Escherichia coli HB101
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.188
NADP+
binding structure at the active site
Homo sapiens
1.1.1.188
NADPH
binding structure at the active site, catalytic structure and mechanism involving the cofactor
Homo sapiens
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
1.1.1.188
20 mg/ml purified recombinant enzyme in a solution containing 1.4 mM PGD2 or inhibitor rutin, 1.2 mM NADP+ and NADPH, 50 mM MES, pH 6.0, and 25% w/v PEG 8000, hanging drop vapour diffusion method, 2-3 days, thick plate-shaped crystals of enzyme with NADP+ and PGD2 and of enzyme with NADPH and rutin, X-ray diffraction structure determination and analysis at 1.69 A resolution
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.188
rutin
binding structure at the substrate binding site
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.188
lung
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.188
additional information
Y55 and H117 are involved in catalysis, enzyme also shows 3alpha-hydroxysteroid dehydrogenase type II activity
654740
Homo sapiens
?
-
-
-
-
1.1.1.188
prostaglandin D2 + NADPH
-
654740
Homo sapiens
9alpha,11beta-prostaglandin F2alpha + NADP+
-
-
-
r
1.1.1.188
prostaglandin H2 + NADPH
-
654740
Homo sapiens
prostaglandin F2alpha + NADP+
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.188
More
enzyme has a (alpha/beta)8 barrel structure, from crystal structure determination
Homo sapiens