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Literature summary extracted from

  • Komoto, J.; Yamada, T.; Watanabe, K.; Takusagawa, F.
    Crystal structure of human prostaglandin F synthase (AKR1C3) (2004), Biochemistry, 43, 2188-2198.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.188 expression in Escherichia coli HB101 Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.188 20 mg/ml purified recombinant enzyme in a solution containing 1.4 mM PGD2 or inhibitor rutin, 1.2 mM NADP+ and NADPH, 50 mM MES, pH 6.0, and 25% w/v PEG 8000, hanging drop vapour diffusion method, 2-3 days, thick plate-shaped crystals of enzyme with NADP+ and PGD2 and of enzyme with NADPH and rutin, X-ray diffraction structure determination and analysis at 1.69 A resolution Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.188 rutin binding structure at the substrate binding site Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.188 Homo sapiens P42330
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.188 (5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP+ = (5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + NADPH + H+ substrate binding and catalytic mechanism, catalytic site residues are Y55 and H117, enzyme also shows 3alpha-hydroxysteroid dehydrogenase type II activity, i.e. 3alpha-HSD Homo sapiens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.188 lung
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.188 additional information Y55 and H117 are involved in catalysis, enzyme also shows 3alpha-hydroxysteroid dehydrogenase type II activity Homo sapiens ?
-
 ?
1.1.1.188 prostaglandin D2 + NADPH + H+
-
 Homo sapiens 9alpha,11beta-prostaglandin F2alpha + NADP+
-
 r
1.1.1.188 prostaglandin H2 + NADPH + H+
-
 Homo sapiens prostaglandin F2alpha + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.188 More enzyme has a (alpha/beta)8 barrel structure, from crystal structure determination Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.1.1.188 AKR1C3
-
 Homo sapiens
1.1.1.188 More enzyme also shows 3alpha-hydroxysteroid dehydrogenase type II activity, i.e. 3alpha-HSD Homo sapiens
1.1.1.188 PGFS
-
 Homo sapiens
1.1.1.188 prostaglandin F synthase
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.188 NADP+ binding structure at the active site Homo sapiens
1.1.1.188 NADPH binding structure at the active site, catalytic structure and mechanism involving the cofactor Homo sapiens