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Literature summary extracted from

  • Wang, H.; Cronan, J.E.
    Only one of the two annotated Lactococcus lactis fabG genes encodes a functional beta-ketoacyl-acyl carrier protein reductase (2004), Biochemistry, 43, 11782-11789.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.100 complementation of Escherichia coli fabG mutant with Lactobacillus lactis fabG genes shows that fabG1 encodes a functional beta-ketoacyl ACP reductase and fabG2 encodes an enzyme that has activity with the model substrate beta-ketobutyryl-CoA, but lacks the ability to support fatty acid synthesis both in vivo and in vitro Lactococcus lactis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.100 Acyl carrier protein beta-ketobutyryl-CoA reductase activity of FabG1 is inhibited, activity of FabG2 is unaffected Lactococcus lactis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.100 0.29
-
 beta-ketobutyryl-CoA pH 7.0, 30°C, FabG1 Lactococcus lactis
1.1.1.100 4.06
-
 beta-ketobutyryl-CoA pH 7.0, 30°C, FabG2 Lactococcus lactis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.100 24000
-
 x * 24000, recombinant methionine-labelled FabG2, SDS-PAGE Lactococcus lactis
1.1.1.100 25000
-
 x * 25000, recombinant methionine-labelled FabG1, SDS-PAGE Lactococcus lactis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.100 additional information Lactococcus lactis FabG1 is responsible for the first reductive step of the fatty acid synthetic cycle. FabG2 is inactive in fatty acid synthesis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.100 Lactococcus lactis
-
 ssp. lactis IL 1403
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.100 beta-ketobutyryl-CoA + NADPH complementation of Escherichia coli fabG mutant with Lactobacillus lactis fabG genes shows that fabG1 encodes a functional beta-ketoacyl ACP reductase and fabG2 encodes an enzyme that has activity with the model substrate beta-ketobutyryl-CoA, but lacks the ability to support fatty acid synthesis both in vivo and in vitro Lactococcus lactis beta-hydroxybutyryl-CoA + NADP+
-
 ?
1.1.1.100 additional information FabG1 is responsible for the first reductive step of the fatty acid synthetic cycle. FabG2 is inactive in fatty acid synthesis Lactococcus lactis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.100 ? x * 24000, recombinant methionine-labelled FabG2, SDS-PAGE Lactococcus lactis
1.1.1.100 ? x * 25000, recombinant methionine-labelled FabG1, SDS-PAGE Lactococcus lactis

Synonyms

EC Number Synonyms Comment Organism
1.1.1.100 FabG1
-
 Lactococcus lactis