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Literature summary extracted from

  • Belyaeva, O.V.; Stetsenko, A.V.; Nelson, P.; Kedishvili, N.Y.
    Properties of short-chain dehydrogenase/reductase RalR1: characterization of purified enzyme, its orientation in the microsomal membrane, and distribution in human tissues and cell lines (2003), Biochemistry, 42, 14838-14845.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.300 production of recombinant His6-tagged enzyme in insect cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.300 R25G/K26I The mutation allows the enzyme to flip its orientation in the membrane. The mutant is glycosylated in intact cells. Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.300 0.00012
-
 all-trans-retinal purified recombinant enzyme Homo sapiens
1.1.1.300 0.0002
-
 all-trans-retinal membrane-bound wild-type Homo sapiens
1.1.1.300 0.0004
-
 NADP+ membrane-bound wild-type Homo sapiens
1.1.1.300 0.00047
-
 NADPH purified recombinant enzyme Homo sapiens
1.1.1.300 0.00048
-
 NADPH membrane-bound wild-type Homo sapiens
1.1.1.300 0.0006
-
 all-trans-retinol purified recombinant enzyme Homo sapiens
1.1.1.300 0.0007
-
 all-trans-retinol membrane-bound wild-type Homo sapiens
1.1.1.300 0.001
-
 NADP+ purified recombinant enzyme Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.300 membrane model of enzyme insertion into the membrane Homo sapiens 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.300 35400
-
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.300 all-trans-retinol + NADP+ Homo sapiens the enzyme plays a role in retinoid metabolism all-trans-retinal + NADPH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.300 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.1.300 no glycoprotein the wild-type enzyme is not glycosylated Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.300 recombinant enzyme Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.300 brain
-
 Homo sapiens
-
1.1.1.300 jejunum
-
 Homo sapiens
-
1.1.1.300 kidney
-
 Homo sapiens
-
1.1.1.300 liver
-
 Homo sapiens
-
1.1.1.300 lung
-
 Homo sapiens
-
1.1.1.300 additional information not: skeletal muscle and heart Homo sapiens
-
1.1.1.300 prostate gland
-
 Homo sapiens
-
1.1.1.300 testis
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.300 0.63
-
 eluate after purification, the specific activity of the purified enzyme with all-trans-retinal as substrate is 13fold higher than that of the microsomal preparation of wild-type Homo sapiens

Storage Stability

EC Number Storage Stability Organism
1.1.1.300 -80°C, buffer containing 1 mM 1,2-diheptanoyl-sn-glycero-3-phosphocholine, stable for several months Homo sapiens
1.1.1.300 8°C, in a refrigerator, the enzyme is nearly fully active for at least one month Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.300 all-trans-retinol + NADP+
-
 Homo sapiens all-trans-retinal + NADPH + H+
-
 r
1.1.1.300 all-trans-retinol + NADP+ the enzyme plays a role in retinoid metabolism Homo sapiens all-trans-retinal + NADPH + H+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.300 RalR1
-
 Homo sapiens
1.1.1.300 retinal reductase 1
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.300 0.2
-
 all-trans-retinol purified recombinant enzyme Homo sapiens
1.1.1.300 0.3
-
 all-trans-retinal purified recombinant enzyme Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.300 NADP+
-
 Homo sapiens
1.1.1.300 NADPH
-
 Homo sapiens