BRENDA - Enzyme Database

Role of active site binding interactions in 4-chlorobenzoyl-coenzyme A dehalogenase catalysis

Luo, L.; Taylor, K.L.; Xiang, H.; Wei, Y.; Zhang, W.; Dunaway-Mariano, D.; Biochemistry 40, 15684-15692 (2001)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.8.1.7
expression of wild-type and mutant enzymes in Escherichia coli
Pseudomonas sp.
Engineering
EC Number
Amino acid exchange
Commentary
Organism
3.8.1.7
F64A
site-directed mutagenesis, decreased kcat and slightly increased Km compared to the wild-type enzyme
Pseudomonas sp.
3.8.1.7
F64P
site-directed mutagenesis, highly decreased kcat compared to the wild-type enzyme, nearly inactive
Pseudomonas sp.
3.8.1.7
G113A
site-directed mutagenesis, decreased kcat and increased Km compared to the wild-type enzyme
Pseudomonas sp.
3.8.1.7
R24K
site-directed mutagenesis, increased kcat and increased Km compared to the wild-type enzyme
Pseudomonas sp.
3.8.1.7
R24L
site-directed mutagenesis, decreased kcat and increased Km compared to the wild-type enzyme
Pseudomonas sp.
3.8.1.7
R257K
site-directed mutagenesis, slightly decreased kcat and slightly increased Km compared to the wild-type enzyme
Pseudomonas sp.
3.8.1.7
R257L
site-directed mutagenesis, decreased kcat and increased Km compared to the wild-type enzyme
Pseudomonas sp.
3.8.1.7
R67K
site-directed mutagenesis, decreased kcat and slightly increased Km compared to the wild-type enzyme
Pseudomonas sp.
3.8.1.7
R67L
site-directed mutagenesis, expression in inclusion bodies
Pseudomonas sp.
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.8.1.7
3'-AMP
weak inhibitor
Pseudomonas sp.
3.8.1.7
3'-dephospho-CoA
-
Pseudomonas sp.
3.8.1.7
4-Chlorobenzoate
poor inhibitor
Pseudomonas sp.
3.8.1.7
4-chlorobenzoyl-coenzyme A
competitive
Pseudomonas sp.
3.8.1.7
4-hydroxybenzoyl-3'-dephospho-CoA
competitive
Pseudomonas sp.
3.8.1.7
4-hydroxybenzoyl-CoA
competitive
Pseudomonas sp.
3.8.1.7
4-hydroxybenzoyl-dithio-CoA
competitive
Pseudomonas sp.
3.8.1.7
4-methylbenzoyl-3'-dephospho-CoA
competitive
Pseudomonas sp.
3.8.1.7
4-methylbenzoyl-CoA
competitive
Pseudomonas sp.
3.8.1.7
5'-ADP
weak inhibitor
Pseudomonas sp.
3.8.1.7
5'-AMP
weak inhibitor
Pseudomonas sp.
3.8.1.7
adenosine
weak inhibitor
Pseudomonas sp.
3.8.1.7
adenosine 3',5'-bisphosphate
weak inhibitor
Pseudomonas sp.
3.8.1.7
ATP
weak inhibitor
Pseudomonas sp.
3.8.1.7
CoA
feedback inhibition
Pseudomonas sp.
3.8.1.7
diphosphate
weak inhibitor
Pseudomonas sp.
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.8.1.7
additional information
-
additional information
kinetics
Pseudomonas sp.
3.8.1.7
0.0037
-
4-chlorobenzoyl-CoA
wild-type enzyme, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.0052
-
4-chlorobenzoyl-CoA
mutant F64A, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.0058
-
4-chlorobenzoyl-CoA
mutant R257K, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.0069
-
4-chlorobenzoyl-CoA
mutant R67K, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.014
-
4-chlorobenzoyl-CoA
mutant G113A, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.02
-
4-chlorobenzoyl-3'-dephospho-CoA
wild-type enzyme, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.021
-
4-chlorobenzoyl-CoA
mutant R24K, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.024
-
4-bromophenacyl-CoA
wild-type enzyme, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.033
-
4-chlorobenzoyl-dithio-CoA
wild-type enzyme, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.041
-
4-chlorobenzoyl-CoA
mutant R24L, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.07
-
4-chlorobenzoyl-CoA
mutant R257L, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.07
-
4-chlorobenzoyl-pantetheine
wild-type enzyme, pH 7.5, 25C, in presence of 1 mM 5'-ADP
Pseudomonas sp.
3.8.1.7
0.11
-
4-chlorobenzoyl-CoA
mutant G114A, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.31
-
4-chlorobenzoyl-epsilon-CoA
wild-type enzyme, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.44
-
4-chlorobenzoyl-pantetheine phosphate
wild-type enzyme, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
2
-
4-chlorobenzoyl-pantetheine
above, wild-type enzyme, pH 7.5, 25C
Pseudomonas sp.
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.8.1.7
4-chlorobenzoyl-CoA + H2O
Pseudomonas sp.
degradation in soil, pathway overview
4-hydroxybenzoyl-CoA + chloride
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.8.1.7
Pseudomonas sp.
-
-
-
Reaction
EC Number
Reaction
Commentary
Organism
3.8.1.7
4-chlorobenzoyl-CoA + H2O = 4-hydroxybenzoyl CoA + chloride
specific for dehalogenation at the 4-position, can dehalogenate substrates bearing fluorine, chlorine, bromine and iodine in the 4-position, multistep mechanism, attack of Asp145 on the C(4) of the substrates' benzoyl ring to form a Meisenheimer intermediate, followed by expulsion of chloride ion to form an arylated enzyme intermediate, and ester hydrolysis to form 4-hydroxybenzoyl-CoA, active site structure
Pseudomonas sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.8.1.7
4-bromophenacyl-CoA + H2O
-
654589
Pseudomonas sp.
4-hydroxyphenacyl-CoA + bromide
-
-
-
?
3.8.1.7
4-chlorobenzoyl-3'-dephospho-CoA + H2O
-
654589
Pseudomonas sp.
4-hydroxybenzoyl-3'-dephospho-CoA + chloride
-
-
-
?
3.8.1.7
4-chlorobenzoyl-CoA + H2O
-
654589
Pseudomonas sp.
4-hydroxybenzoyl-CoA + chloride
-
-
-
?
3.8.1.7
4-chlorobenzoyl-CoA + H2O
degradation in soil, pathway overview
654589
Pseudomonas sp.
4-hydroxybenzoyl-CoA + chloride
-
-
-
?
3.8.1.7
4-chlorobenzoyl-dithio-CoA + H2O
-
654589
Pseudomonas sp.
4-hydroxybenzoyl-dithio-CoA + chloride
-
-
-
?
3.8.1.7
4-chlorobenzoyl-epsilon-CoA + H2O
-
654589
Pseudomonas sp.
4-hydroxybenzoyl-epsilon-CoA + chloride
-
-
-
?
3.8.1.7
4-chlorobenzoyl-pantetheine + H2O
very low activity
654589
Pseudomonas sp.
4-hydroxybenzoyl-pantetheine + chloride
-
-
-
?
3.8.1.7
4-chlorobenzoyl-pantetheine phosphate + H2O
-
654589
Pseudomonas sp.
4-hydroxybenzoyl-pantetheine phosphate + chloride
-
-
-
?
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.8.1.7
25
-
assay at
Pseudomonas sp.
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.8.1.7
0.00001
-
4-chlorobenzoyl-CoA
below, mutant F64P, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.001
-
4-chlorobenzoyl-pantetheine
about, wild-type enzyme, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.002
-
4-chlorobenzoyl-CoA
mutant G114A, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.003
-
4-chlorobenzoyl-dithio-CoA
wild-type enzyme, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.005
-
4-chlorobenzoyl-CoA
mutant G113A, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.007
-
4-chlorobenzoyl-epsilon-CoA
wild-type enzyme, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.03
-
4-bromophenacyl-CoA
wild-type enzyme, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.03
-
4-chlorobenzoyl-CoA
mutant F64A, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.051
-
4-chlorobenzoyl-pantetheine phosphate
wild-type enzyme, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.056
-
4-chlorobenzoyl-pantetheine
wild-type enzyme, pH 7.5, 25C, in presence of 1 mM 5'-ADP
Pseudomonas sp.
3.8.1.7
0.14
-
4-chlorobenzoyl-CoA
mutant R257L, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.33
-
4-chlorobenzoyl-CoA
mutant R24L, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.38
-
4-chlorobenzoyl-CoA
mutant R67K, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.45
-
4-chlorobenzoyl-3'-dephospho-CoA
wild-type enzyme, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.55
-
4-chlorobenzoyl-CoA
mutant R257K, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.6
-
4-chlorobenzoyl-CoA
wild-type enzyme, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.95
-
4-chlorobenzoyl-CoA
mutant R24K, pH 7.5, 25C
Pseudomonas sp.
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.8.1.7
7.5
-
assay at
Pseudomonas sp.
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.8.1.7
0.0017
-
4-hydroxybenzoyl-CoA
mutant R257K, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.0024
-
4-hydroxybenzoyl-CoA
mutant R24K, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.0025
-
4-hydroxybenzoyl-CoA
wild-type enzyme, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.0031
-
4-methylbenzoyl-CoA
mutant R257K, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.0042
-
4-methylbenzoyl-CoA
wild-type enzyme, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.0062
-
4-hydroxybenzoyl-CoA
mutant R67K, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.011
-
4-methylbenzoyl-CoA
mutant R24K, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.0165
-
4-hydroxybenzoyl-CoA
mutant R24L, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.019
-
4-hydroxybenzoyl-CoA
mutant R257L, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.022
-
4-methylbenzoyl-3'-dephospho-CoA
wild-type enzyme, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.04
-
4-hydroxybenzoyl-CoA
mutant G113A, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.044
-
4-methylbenzoyl-CoA
mutant G113A, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.046
-
4-methylbenzoyl-CoA
mutant R24L, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.05
0.1
4-hydroxybenzoyl-CoA
mutant G114A, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.051
-
4-methylbenzoyl-CoA
mutant R67K, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.055
-
4-chlorobenzoyl-coenzyme A
wild-type enzyme, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.069
-
4-hydroxybenzoyl-dithio-CoA
wild-type enzyme, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.072
-
4-methylbenzoyl-CoA
mutant R257L, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.09
-
CoA
mutant R257K, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.14
-
CoA
wild-type enzyme, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.25
-
CoA
mutant R24K, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.52
-
CoA
mutant R67K, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.66
-
CoA
mutant R24L, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.77
-
3'-dephospho-CoA
wild-type enzyme, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
2.4
-
CoA
mutant R257L, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
4.2
-
5'-ADP
wild-type enzyme, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
21
-
4-Chlorobenzoate
wild-type enzyme, pH 7.5, 25C
Pseudomonas sp.
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.8.1.7
expression of wild-type and mutant enzymes in Escherichia coli
Pseudomonas sp.
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
3.8.1.7
F64A
site-directed mutagenesis, decreased kcat and slightly increased Km compared to the wild-type enzyme
Pseudomonas sp.
3.8.1.7
F64P
site-directed mutagenesis, highly decreased kcat compared to the wild-type enzyme, nearly inactive
Pseudomonas sp.
3.8.1.7
G113A
site-directed mutagenesis, decreased kcat and increased Km compared to the wild-type enzyme
Pseudomonas sp.
3.8.1.7
R24K
site-directed mutagenesis, increased kcat and increased Km compared to the wild-type enzyme
Pseudomonas sp.
3.8.1.7
R24L
site-directed mutagenesis, decreased kcat and increased Km compared to the wild-type enzyme
Pseudomonas sp.
3.8.1.7
R257K
site-directed mutagenesis, slightly decreased kcat and slightly increased Km compared to the wild-type enzyme
Pseudomonas sp.
3.8.1.7
R257L
site-directed mutagenesis, decreased kcat and increased Km compared to the wild-type enzyme
Pseudomonas sp.
3.8.1.7
R67K
site-directed mutagenesis, decreased kcat and slightly increased Km compared to the wild-type enzyme
Pseudomonas sp.
3.8.1.7
R67L
site-directed mutagenesis, expression in inclusion bodies
Pseudomonas sp.
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.8.1.7
3'-AMP
weak inhibitor
Pseudomonas sp.
3.8.1.7
3'-dephospho-CoA
-
Pseudomonas sp.
3.8.1.7
4-Chlorobenzoate
poor inhibitor
Pseudomonas sp.
3.8.1.7
4-chlorobenzoyl-coenzyme A
competitive
Pseudomonas sp.
3.8.1.7
4-hydroxybenzoyl-3'-dephospho-CoA
competitive
Pseudomonas sp.
3.8.1.7
4-hydroxybenzoyl-CoA
competitive
Pseudomonas sp.
3.8.1.7
4-hydroxybenzoyl-dithio-CoA
competitive
Pseudomonas sp.
3.8.1.7
4-methylbenzoyl-3'-dephospho-CoA
competitive
Pseudomonas sp.
3.8.1.7
4-methylbenzoyl-CoA
competitive
Pseudomonas sp.
3.8.1.7
5'-ADP
weak inhibitor
Pseudomonas sp.
3.8.1.7
5'-AMP
weak inhibitor
Pseudomonas sp.
3.8.1.7
adenosine
weak inhibitor
Pseudomonas sp.
3.8.1.7
adenosine 3',5'-bisphosphate
weak inhibitor
Pseudomonas sp.
3.8.1.7
ATP
weak inhibitor
Pseudomonas sp.
3.8.1.7
CoA
feedback inhibition
Pseudomonas sp.
3.8.1.7
diphosphate
weak inhibitor
Pseudomonas sp.
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.8.1.7
0.0017
-
4-hydroxybenzoyl-CoA
mutant R257K, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.0024
-
4-hydroxybenzoyl-CoA
mutant R24K, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.0025
-
4-hydroxybenzoyl-CoA
wild-type enzyme, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.0031
-
4-methylbenzoyl-CoA
mutant R257K, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.0042
-
4-methylbenzoyl-CoA
wild-type enzyme, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.0062
-
4-hydroxybenzoyl-CoA
mutant R67K, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.011
-
4-methylbenzoyl-CoA
mutant R24K, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.0165
-
4-hydroxybenzoyl-CoA
mutant R24L, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.019
-
4-hydroxybenzoyl-CoA
mutant R257L, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.022
-
4-methylbenzoyl-3'-dephospho-CoA
wild-type enzyme, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.04
-
4-hydroxybenzoyl-CoA
mutant G113A, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.044
-
4-methylbenzoyl-CoA
mutant G113A, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.046
-
4-methylbenzoyl-CoA
mutant R24L, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.05
0.1
4-hydroxybenzoyl-CoA
mutant G114A, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.051
-
4-methylbenzoyl-CoA
mutant R67K, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.055
-
4-chlorobenzoyl-coenzyme A
wild-type enzyme, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.069
-
4-hydroxybenzoyl-dithio-CoA
wild-type enzyme, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.072
-
4-methylbenzoyl-CoA
mutant R257L, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.09
-
CoA
mutant R257K, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.14
-
CoA
wild-type enzyme, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.25
-
CoA
mutant R24K, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.52
-
CoA
mutant R67K, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.66
-
CoA
mutant R24L, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.77
-
3'-dephospho-CoA
wild-type enzyme, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
2.4
-
CoA
mutant R257L, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
4.2
-
5'-ADP
wild-type enzyme, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
21
-
4-Chlorobenzoate
wild-type enzyme, pH 7.5, 25C
Pseudomonas sp.
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.8.1.7
additional information
-
additional information
kinetics
Pseudomonas sp.
3.8.1.7
0.0037
-
4-chlorobenzoyl-CoA
wild-type enzyme, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.0052
-
4-chlorobenzoyl-CoA
mutant F64A, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.0058
-
4-chlorobenzoyl-CoA
mutant R257K, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.0069
-
4-chlorobenzoyl-CoA
mutant R67K, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.014
-
4-chlorobenzoyl-CoA
mutant G113A, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.02
-
4-chlorobenzoyl-3'-dephospho-CoA
wild-type enzyme, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.021
-
4-chlorobenzoyl-CoA
mutant R24K, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.024
-
4-bromophenacyl-CoA
wild-type enzyme, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.033
-
4-chlorobenzoyl-dithio-CoA
wild-type enzyme, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.041
-
4-chlorobenzoyl-CoA
mutant R24L, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.07
-
4-chlorobenzoyl-CoA
mutant R257L, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.07
-
4-chlorobenzoyl-pantetheine
wild-type enzyme, pH 7.5, 25C, in presence of 1 mM 5'-ADP
Pseudomonas sp.
3.8.1.7
0.11
-
4-chlorobenzoyl-CoA
mutant G114A, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.31
-
4-chlorobenzoyl-epsilon-CoA
wild-type enzyme, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.44
-
4-chlorobenzoyl-pantetheine phosphate
wild-type enzyme, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
2
-
4-chlorobenzoyl-pantetheine
above, wild-type enzyme, pH 7.5, 25C
Pseudomonas sp.
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.8.1.7
4-chlorobenzoyl-CoA + H2O
Pseudomonas sp.
degradation in soil, pathway overview
4-hydroxybenzoyl-CoA + chloride
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.8.1.7
4-bromophenacyl-CoA + H2O
-
654589
Pseudomonas sp.
4-hydroxyphenacyl-CoA + bromide
-
-
-
?
3.8.1.7
4-chlorobenzoyl-3'-dephospho-CoA + H2O
-
654589
Pseudomonas sp.
4-hydroxybenzoyl-3'-dephospho-CoA + chloride
-
-
-
?
3.8.1.7
4-chlorobenzoyl-CoA + H2O
-
654589
Pseudomonas sp.
4-hydroxybenzoyl-CoA + chloride
-
-
-
?
3.8.1.7
4-chlorobenzoyl-CoA + H2O
degradation in soil, pathway overview
654589
Pseudomonas sp.
4-hydroxybenzoyl-CoA + chloride
-
-
-
?
3.8.1.7
4-chlorobenzoyl-dithio-CoA + H2O
-
654589
Pseudomonas sp.
4-hydroxybenzoyl-dithio-CoA + chloride
-
-
-
?
3.8.1.7
4-chlorobenzoyl-epsilon-CoA + H2O
-
654589
Pseudomonas sp.
4-hydroxybenzoyl-epsilon-CoA + chloride
-
-
-
?
3.8.1.7
4-chlorobenzoyl-pantetheine + H2O
very low activity
654589
Pseudomonas sp.
4-hydroxybenzoyl-pantetheine + chloride
-
-
-
?
3.8.1.7
4-chlorobenzoyl-pantetheine phosphate + H2O
-
654589
Pseudomonas sp.
4-hydroxybenzoyl-pantetheine phosphate + chloride
-
-
-
?
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.8.1.7
25
-
assay at
Pseudomonas sp.
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.8.1.7
0.00001
-
4-chlorobenzoyl-CoA
below, mutant F64P, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.001
-
4-chlorobenzoyl-pantetheine
about, wild-type enzyme, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.002
-
4-chlorobenzoyl-CoA
mutant G114A, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.003
-
4-chlorobenzoyl-dithio-CoA
wild-type enzyme, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.005
-
4-chlorobenzoyl-CoA
mutant G113A, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.007
-
4-chlorobenzoyl-epsilon-CoA
wild-type enzyme, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.03
-
4-bromophenacyl-CoA
wild-type enzyme, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.03
-
4-chlorobenzoyl-CoA
mutant F64A, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.051
-
4-chlorobenzoyl-pantetheine phosphate
wild-type enzyme, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.056
-
4-chlorobenzoyl-pantetheine
wild-type enzyme, pH 7.5, 25C, in presence of 1 mM 5'-ADP
Pseudomonas sp.
3.8.1.7
0.14
-
4-chlorobenzoyl-CoA
mutant R257L, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.33
-
4-chlorobenzoyl-CoA
mutant R24L, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.38
-
4-chlorobenzoyl-CoA
mutant R67K, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.45
-
4-chlorobenzoyl-3'-dephospho-CoA
wild-type enzyme, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.55
-
4-chlorobenzoyl-CoA
mutant R257K, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.6
-
4-chlorobenzoyl-CoA
wild-type enzyme, pH 7.5, 25C
Pseudomonas sp.
3.8.1.7
0.95
-
4-chlorobenzoyl-CoA
mutant R24K, pH 7.5, 25C
Pseudomonas sp.
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.8.1.7
7.5
-
assay at
Pseudomonas sp.