EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
6.3.4.14 | biotin | activates the ATP synthesis reaction with ADP and carbamoyl phosphate as substrates | Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
6.3.4.14 | C230A | kinetic data, 50fold increased Km for ATP, no effect on the formation of carboxybiotin | Escherichia coli |
6.3.4.14 | K238Q | kinetic data, 50fold increased Km for ATP, no formation of carboxybiotin | Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
6.3.4.14 | N-ethylmaleimide | pH-dependent inhibition, reacts with Lys-238 | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.4.14 | additional information | - |
additional information | - |
Escherichia coli | |
6.3.4.14 | 0.081 | - |
ATP | 25°C, wild-type enzyme, bicarbonate-dependent ATP hydrolysis in the absence of biotin | Escherichia coli | |
6.3.4.14 | 0.37 | - |
HCO3- | 25°C, wild-type enzyme, bicarbonate-dependent ATP hydrolysis in the absence of biotin | Escherichia coli | |
6.3.4.14 | 0.7 | - |
HCO3- | 25°C, C230A mutant, bicarbonate-dependent ATP hydrolysis in the absence of biotin | Escherichia coli | |
6.3.4.14 | 4.23 | - |
ATP | 25°C, C230A mutant, bicarbonate-dependent ATP hydrolysis in the absence of biotin | Escherichia coli | |
6.3.4.14 | 6.87 | - |
ATP | 25°C, K238Q mutant, bicarbonate-dependent ATP hydrolysis in the absence of biotin | Escherichia coli | |
6.3.4.14 | 122 | - |
biotin | 25°C, free biotin, wild-type enzyme | Escherichia coli | |
6.3.4.14 | 167 | - |
biotin | 25°C, free biotin, C230A mutant | Escherichia coli |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
6.3.4.14 | Mg2+ | requirement | Escherichia coli |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
6.3.4.14 | 50000 | - |
2 * 50000 | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.4.14 | additional information | Escherichia coli | long-chain fatty acid synthesis | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.3.4.14 | Escherichia coli | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.3.4.14 | wild-type and mutant enzymes | Escherichia coli |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
6.3.4.14 | ATP + [biotin carboxyl-carrier protein]-biotin-N6-L-lysine + hydrogencarbonate- = ADP + phosphate + [biotin carboxyl-carrier protein]-carboxybiotin-N6-L-lysine | mechanism | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.4.14 | ADP + carbamoyl phosphate | biotin carboxylase catalyzes an ATP synthesis reaction, in which a phosphate group is transferred from carbamoyl phosphate to ADP forming ATP and carbamate | Escherichia coli | ATP + carbamate | carbamate rapidly decomposes into carbon dioxide and ammonia | ? | |
6.3.4.14 | ATP + biotin + HCO3- | also utilizes free biotin as substrate | Escherichia coli | ADP + phosphate + carboxybiotin | - |
? | |
6.3.4.14 | ATP + biotin-carboxyl-carrier protein + HCO3- | one component of the multienzyme complex acetyl-CoA carboxylase, catalyzes the ATP-dependent carboxylation of biotin, for that biotin must be deprotonated at its N1 position, mechanism for deprotonation of biotin, bicarbonate is the source of CO2, Lys-238 plays a role in the carboxylation reaction, Cys-230 and Lys-238 do not act as an acid-base pair in the deprotonation of biotin, but may be involved in ATP binding | Escherichia coli | ADP + phosphate + carboxybiotin-carboxyl-carrier protein | - |
r | |
6.3.4.14 | additional information | long-chain fatty acid synthesis | Escherichia coli | ? | - |
? | |
6.3.4.14 | additional information | biotin carboxylase catalyzes a slow bicarbonate-dependent ATP hydrolysis reaction in the absence of biotin | Escherichia coli | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.3.4.14 | homodimer | 2 * 50000 | Escherichia coli |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.3.4.14 | 25 | - |
assay at | Escherichia coli |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.4.14 | additional information | - |
additional information | - |
Escherichia coli | |
6.3.4.14 | 0.0012 | - |
ATP | 25°C, wild-type enzyme, bicarbonate-dependent ATP hydrolysis in the absence of biotin | Escherichia coli | |
6.3.4.14 | 0.0017 | - |
ATP | 25°C, C230A mutant, bicarbonate-dependent ATP hydrolysis in the absence of biotin | Escherichia coli | |
6.3.4.14 | 0.00195 | - |
ATP | 25°C, K238Q mutant, bicarbonate-dependent ATP hydrolysis in the absence of biotin | Escherichia coli | |
6.3.4.14 | 0.513 | - |
ATP | 25°C, C230A mutant, biotin-dependent ATP hydrolysis | Escherichia coli | |
6.3.4.14 | 1.23 | - |
ATP | 25°C, wild-type enzyme, biotin-dependent ATP hydrolysis | Escherichia coli |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
6.3.4.14 | ATP | ATP-dependent, Cys-230 and Lys-238 may be involved in binding ATP | Escherichia coli |