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Literature summary extracted from

  • Levert, K.L.; Lloyd, R.B.; Waldrop, G.L.
    Do cysteine 230 and lysine 238 of biotin carboxylase play a role in the activation of biotin? (2000), Biochemistry, 39, 4122-4128.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.3.4.14 biotin activates the ATP synthesis reaction with ADP and carbamoyl phosphate as substrates Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
6.3.4.14 C230A kinetic data, 50fold increased Km for ATP, no effect on the formation of carboxybiotin Escherichia coli
6.3.4.14 K238Q kinetic data, 50fold increased Km for ATP, no formation of carboxybiotin Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.4.14 N-ethylmaleimide pH-dependent inhibition, reacts with Lys-238 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.4.14 additional information
-
additional information
-
Escherichia coli
6.3.4.14 0.081
-
ATP 25°C, wild-type enzyme, bicarbonate-dependent ATP hydrolysis in the absence of biotin Escherichia coli
6.3.4.14 0.37
-
HCO3- 25°C, wild-type enzyme, bicarbonate-dependent ATP hydrolysis in the absence of biotin Escherichia coli
6.3.4.14 0.7
-
HCO3- 25°C, C230A mutant, bicarbonate-dependent ATP hydrolysis in the absence of biotin Escherichia coli
6.3.4.14 4.23
-
ATP 25°C, C230A mutant, bicarbonate-dependent ATP hydrolysis in the absence of biotin Escherichia coli
6.3.4.14 6.87
-
ATP 25°C, K238Q mutant, bicarbonate-dependent ATP hydrolysis in the absence of biotin Escherichia coli
6.3.4.14 122
-
biotin 25°C, free biotin, wild-type enzyme Escherichia coli
6.3.4.14 167
-
biotin 25°C, free biotin, C230A mutant Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.4.14 Mg2+ requirement Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.4.14 50000
-
2 * 50000 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.4.14 additional information Escherichia coli long-chain fatty acid synthesis ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.14 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.14 wild-type and mutant enzymes Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
6.3.4.14 ATP + [biotin carboxyl-carrier protein]-biotin-N6-L-lysine + hydrogencarbonate- = ADP + phosphate + [biotin carboxyl-carrier protein]-carboxybiotin-N6-L-lysine mechanism Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.14 ADP + carbamoyl phosphate biotin carboxylase catalyzes an ATP synthesis reaction, in which a phosphate group is transferred from carbamoyl phosphate to ADP forming ATP and carbamate Escherichia coli ATP + carbamate carbamate rapidly decomposes into carbon dioxide and ammonia ?
6.3.4.14 ATP + biotin + HCO3- also utilizes free biotin as substrate Escherichia coli ADP + phosphate + carboxybiotin
-
?
6.3.4.14 ATP + biotin-carboxyl-carrier protein + HCO3- one component of the multienzyme complex acetyl-CoA carboxylase, catalyzes the ATP-dependent carboxylation of biotin, for that biotin must be deprotonated at its N1’ position, mechanism for deprotonation of biotin, bicarbonate is the source of CO2, Lys-238 plays a role in the carboxylation reaction, Cys-230 and Lys-238 do not act as an acid-base pair in the deprotonation of biotin, but may be involved in ATP binding Escherichia coli ADP + phosphate + carboxybiotin-carboxyl-carrier protein
-
r
6.3.4.14 additional information long-chain fatty acid synthesis Escherichia coli ?
-
?
6.3.4.14 additional information biotin carboxylase catalyzes a slow bicarbonate-dependent ATP hydrolysis reaction in the absence of biotin Escherichia coli ?
-
?

Subunits

EC Number Subunits Comment Organism
6.3.4.14 homodimer 2 * 50000 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.4.14 25
-
assay at Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.4.14 additional information
-
additional information
-
Escherichia coli
6.3.4.14 0.0012
-
ATP 25°C, wild-type enzyme, bicarbonate-dependent ATP hydrolysis in the absence of biotin Escherichia coli
6.3.4.14 0.0017
-
ATP 25°C, C230A mutant, bicarbonate-dependent ATP hydrolysis in the absence of biotin Escherichia coli
6.3.4.14 0.00195
-
ATP 25°C, K238Q mutant, bicarbonate-dependent ATP hydrolysis in the absence of biotin Escherichia coli
6.3.4.14 0.513
-
ATP 25°C, C230A mutant, biotin-dependent ATP hydrolysis Escherichia coli
6.3.4.14 1.23
-
ATP 25°C, wild-type enzyme, biotin-dependent ATP hydrolysis Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.4.14 ATP ATP-dependent, Cys-230 and Lys-238 may be involved in binding ATP Escherichia coli