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Literature summary extracted from
- Enhancement of the enzymatic activity of ribonuclease HI from Thermus thermophilus HB8 with a suppressor mutation method (2000), Biochemistry, 39, 13285-13294.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.26.4 | gene rnhA, overexpression in Escherichia coli MIC3001 of wild-type and mutant enzymes, complementation of enzyme-dependent temperature-sensitive growth phenotype of the Escherichia coli host strain by expression of the wild-type enzyme, but only marginally by the mutant D134H | Thermus thermophilus |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.1.26.4 | A12S | random mutagenesis, roughly cumulative effect on enzyme activity, increased kcat/Km value compared to the wild-type enzyme | Thermus thermophilus |
| 3.1.26.4 | A12S/K75M/A77P | site-directed mutagenesis, 40fold increased kcat/Km value compared to the wild-type enzyme, thermal stability similar to the wild-type enzyme | Thermus thermophilus |
| 3.1.26.4 | A77P | random mutagenesis, roughly cumulative effect on enzyme activity, increased kcat/Km value compared to the wild-type enzyme | Thermus thermophilus |
| 3.1.26.4 | D134H | site-directed mutagenesis, active site mutant, highly reduced catalytic activity | Thermus thermophilus |
| 3.1.26.4 | D134N | site-directed mutagenesis, active site mutant, similar to the wild-type enzyme | Thermus thermophilus |
| 3.1.26.4 | K75M | random mutagenesis, roughly cumulative effect on enzyme activity, increased kcat/Km value compared to the wild-type enzyme | Thermus thermophilus |
| 3.1.26.4 | additional information | isolation of a mutant enzyme with enhanced activity at moderate temperatures, genetic method development | Thermus thermophilus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.26.4 | additional information | - |
additional information | thermodynamics of wild-type and mutant enzymes | Thermus thermophilus | |
| 3.1.26.4 | 0.0011 | - |
DNA-RNA-DNA hybrid | mutant A12S/K75M and A12S/K75M/A77P, pH 8.0, 30°C | Thermus thermophilus | |
| 3.1.26.4 | 0.0013 | - |
DNA-RNA-DNA hybrid | mutant A12S/A77P, pH 8.0, 30°C | Thermus thermophilus | |
| 3.1.26.4 | 0.0015 | - |
DNA-RNA-DNA hybrid | mutant A12S, pH 8.0, 30°C | Thermus thermophilus | |
| 3.1.26.4 | 0.002 | - |
DNA-RNA-DNA hybrid | mutant K75M/A77P, pH 8.0, 30°C | Thermus thermophilus | |
| 3.1.26.4 | 0.0033 | - |
DNA-RNA-DNA hybrid | mutant D134H and A77P, pH 8.0, 30°C | Thermus thermophilus | |
| 3.1.26.4 | 0.0061 | - |
DNA-RNA-DNA hybrid | mutant K75M, pH 8.0, 30°C | Thermus thermophilus | |
| 3.1.26.4 | 0.0078 | - |
DNA-RNA-DNA hybrid | wild-type enzyme, pH 8.0, 30°C | Thermus thermophilus |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.26.4 | Mg2+ | - |
Thermus thermophilus |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.26.4 | Thermus thermophilus | - |
type I enzyme, gene rnhA | - |
| 3.1.26.4 | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
type I enzyme, gene rnhA | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.26.4 | recombinant wild-type and mutant enzymes from Escherichia coli | Thermus thermophilus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.26.4 | additional information | - |
activity of wild-type and mutant enzymes at different temperatures | Thermus thermophilus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.26.4 | DNA-RNA-DNA hybrid + H2O | specific hydrolysis of the RNA strand of the hybrid | Thermus thermophilus | ? | - |
? | |
| 3.1.26.4 | DNA-RNA-DNA hybrid + H2O | specific hydrolysis of the RNA strand of the hybrid | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.26.4 | ribonuclease HI | - |
Thermus thermophilus |
| 3.1.26.4 | RNase HI | - |
Thermus thermophilus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.26.4 | 30 | - |
assay at | Thermus thermophilus |
| 3.1.26.4 | 60 | - |
assay at | Thermus thermophilus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.26.4 | 0.003 | - |
DNA-RNA-DNA hybrid | mutant D134H, pH 8.0, 30°C | Thermus thermophilus | |
| 3.1.26.4 | 0.075 | - |
DNA-RNA-DNA hybrid | mutant A12S, pH 8.0, 30°C | Thermus thermophilus | |
| 3.1.26.4 | 0.082 | - |
DNA-RNA-DNA hybrid | wild-type enzyme, pH 8.0, 30°C | Thermus thermophilus | |
| 3.1.26.4 | 0.122 | - |
DNA-RNA-DNA hybrid | mutant A77P, pH 8.0, 30°C | Thermus thermophilus | |
| 3.1.26.4 | 0.133 | - |
DNA-RNA-DNA hybrid | mutant K75M, pH 8.0, 30°C | Thermus thermophilus | |
| 3.1.26.4 | 0.2 | - |
DNA-RNA-DNA hybrid | mutant A12S/K75M, pH 8.0, 30°C | Thermus thermophilus | |
| 3.1.26.4 | 0.25 | - |
DNA-RNA-DNA hybrid | mutant A12S/A77P, pH 8.0, 30°C | Thermus thermophilus | |
| 3.1.26.4 | 0.33 | - |
DNA-RNA-DNA hybrid | mutant K75M/A77P, pH 8.0, 30°C | Thermus thermophilus | |
| 3.1.26.4 | 0.45 | - |
DNA-RNA-DNA hybrid | mutant A12S/K75M/A77P, pH 8.0, 30°C | Thermus thermophilus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.26.4 | 8 | - |
assay at | Thermus thermophilus |
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