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Literature summary extracted from

  • Gagnon, I.; Duester, G.; Bhat, P.V.
    Enzymatic characterization of recombinant mouse retinal dehydrogenase type 1 (2003), Biochem. Pharmacol., 65, 1685-1690.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.36 expression of isozyme type-1 in Escherichia coli strain BL21(DE3) as N-terminally fusion protein to glutathione-S-transferase Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.36 acetaldehyde 60% competitive inhibition of all-trans-retinal oxidation at 0.4 mM Mus musculus
1.2.1.36 Chloral hydrate 80% competitive inhibition of all-trans-retinal oxidation at 0.1 mM Mus musculus
1.2.1.36 Mg2+ about 50% inhibition of all-trans- and 9-cis-retinal oxidation above 1 mM Mus musculus
1.2.1.36 retinol 40% uncompetitive inhibition of all-trans-retinal oxidation at 0.012 mM Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.36 0.0036
-
9-cis-retinal recombinant isozyme type-1, pH 7.5, 25°C Mus musculus
1.2.1.36 0.0116
-
all-trans-retinal recombinant isozyme type-1, pH 7.5, 25°C Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.36 55000
-
x * 55000, recombinant detagged isozyme type-1, SDS-PAGE Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.36 9-cis-retinal + NAD+ + H2O Mus musculus
-
9-cis-retinoate + NADH
-
?
1.2.1.36 all-trans-retinal + NAD+ + H2O Mus musculus
-
all-trans-retinoate + NADH
-
?
1.2.1.36 additional information Mus musculus enzyme is involved in retinoic acids biosynthesis, which are important signaling molecules in embryogenesis and tissue differentiation ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.36 Mus musculus
-
isozyme type-1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.36 recombinant isozyme type-1 as fusion protein from Escherichia coli by GST-affinity chromatography, cleavage by thrombin to eliminate the fusion tag Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.36 embryo
-
Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.36 9-cis-retinal + NAD+ + H2O
-
Mus musculus 9-cis-retinoate + NADH
-
?
1.2.1.36 all-trans-retinal + NAD+ + H2O
-
Mus musculus all-trans-retinoate + NADH
-
?
1.2.1.36 all-trans-retinal + NAD+ + H2O preferred substrate of recombinant isozyme type-1 Mus musculus all-trans-retinoate + NADH
-
?
1.2.1.36 additional information enzyme is involved in retinoic acids biosynthesis, which are important signaling molecules in embryogenesis and tissue differentiation Mus musculus ?
-
?
1.2.1.36 additional information substrate specificities at different substrate concentrations, no activity with 13-cis-retinal Mus musculus ?
-
?

Subunits

EC Number Subunits Comment Organism
1.2.1.36 ? x * 55000, recombinant detagged isozyme type-1, SDS-PAGE Mus musculus

Synonyms

EC Number Synonyms Comment Organism
1.2.1.36 RalDH1
-
Mus musculus
1.2.1.36 retinaldehyde dehydrogenase type-1
-
Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.36 25
-
assay at Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.36 7.8
-
recombinant isozyme type-1 exhibits 2 pH-optima at pH 7.8 and pH 9.4 with substrates all-trans-retinal and 9-cis-retinal, respectively Mus musculus
1.2.1.36 9.4
-
recombinant isozyme type-1 exhibits 2 pH-optima at pH 7.8 and pH 9.4 with substrates all-trans-retinal and 9-cis-retinal, respectively Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.36 NAD+
-
Mus musculus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.2.1.36 additional information
-
additional information kinetics, recombinant isozyme type-1 Mus musculus
1.2.1.36 0.005
-
Chloral hydrate recombinant isozyme type-1, pH 7.5, 25°C, with substrate all-trans-retinal Mus musculus
1.2.1.36 0.049
-
acetaldehyde recombinant isozyme type-1, pH 7.5, 25°C, with substrate all-trans-retinal Mus musculus