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Literature summary extracted from

  • Roberts, S.J.; Stewart, A.J.; Sadler, P.J.; Farquharson, C.
    Human PHOSPHO1 exhibits high specific phosphoethanolamine and phosphocholine phosphatase activities (2004), Biochem. J., 382, 59-65.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.1.3.75 medicine may have implications for the diagnosis of hypophosphatasia and treatment of bone mineralization abnormalities such as osteomalacia and pathological soft-tissue ossification, a process clinically significant in atheroscleosis and heart failure Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.75 expression in Escherichia coli TOP10 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.75 0.003
-
 O-Phosphoethanolamine pH 6.7, 37°C, 2 mM Mg2+ Homo sapiens
3.1.3.75 0.0114
-
 phosphocholine pH 6.7, 37°C, 2 mM Mg2+ Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.75 Co2+ stimulates to a lesser extend than Mg2+, higher activity with phosphocholine than with phosphoethanolamine in the presence of Co2+ and Mn2+ most probably due to an allosteric effect caused by a difference in the metal-binding properties of each enzyme-substrate complex Homo sapiens
3.1.3.75 Mg2+ high specific Mg2+-dependence, optimum concentration: 2 mM MgCl2 Homo sapiens
3.1.3.75 Mn2+ stimulates to a lesser extend than Mg2+, higher activity with phosphocholine than with phosphoethanolamine in the presence of Co2+ and Mn2+ most probably due to an allosteric effect caused by a difference in the metal-binding properties of each enzyme-substrate complex Homo sapiens
3.1.3.75 additional information not activated by Ca2+ or Zn2+ Homo sapiens
3.1.3.75 Ni2+ stimulates to a lesser extend than Mg2+ Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.75 32000
-
 x * 32000, recombinant PHOSPHO1, SDS-PAGE, Western blot analysis Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.75 O-phosphoethanolamine + H2O Homo sapiens likely a natural substrate, phosphoethanolamine metabolism, PHOSPHO1 is upregulated in mineralizing cells, enzyme is implicated in the generation of phosphate for matrix mineralization ethanolamine + phosphate
-
 ?
3.1.3.75 phosphocholine + H2O Homo sapiens likely a natural substrate, phosphocholine metabolism, PHOSPHO1 is upregulated in mineralizing cells, enzyme is implicated in the generation of phosphate for matrix mineralization choline + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.75 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.75 recombinant PHOSPHO1 Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.3.75 O-phosphoethanolamine + H2O = ethanolamine + phosphate requires active site Mg2+ but also works, to a lesser extend, with Co2+ and Mn2+, the enzyme is highly specific for phosphoethanolamine and phosphocholine Homo sapiens
a
3.1.3.75 phosphocholine + H2O = choline + phosphate requires active site Mg2+ but also works, to a lesser extend, with Co2+ and Mn2+, the enzyme is highly specific for phosphoethanolamine and phosphocholine Homo sapiens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.75 SAOS-2 cell osteoblast-like cells Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.75 0.0176
-
 pH 7.2, 37°C, hydrolysis of pyridoxal 5’-phosphate Homo sapiens
3.1.3.75 0.0396
-
 pH 7.2, 37°C, hydrolysis of beta-glycerol phosphate Homo sapiens
3.1.3.75 0.0645
-
 pH 7.2, 37°C, hydrolysis of p-nitrophenyl phosphate Homo sapiens
3.1.3.75 0.0748
-
 pH 7.2, 37°C, hydrolysis of ribose 5-phosphate Homo sapiens
3.1.3.75 2.98
-
 pH 7.2, 37°C, hydrolysis of phosphocholine Homo sapiens
3.1.3.75 4.6
-
 pH 7.2, 37°C, hydrolysis of phosphoethanolamine Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.75 beta-glycerol phosphate + H2O poor substrate Homo sapiens glycerol + phosphate
-
 ?
3.1.3.75 D-ribose 5-phosphate + H2O poor substrate Homo sapiens D-ribose + phosphate
-
 ?
3.1.3.75 additional information not: diphosphate, phospho-L-serine, glycone phosphate, fructose 6-phosphate, phospho-L-tyrosine, ATP Homo sapiens ?
-
 ?
3.1.3.75 O-phosphoethanolamine + H2O likely a natural substrate, phosphoethanolamine metabolism, PHOSPHO1 is upregulated in mineralizing cells, enzyme is implicated in the generation of phosphate for matrix mineralization Homo sapiens ethanolamine + phosphate
-
 ?
3.1.3.75 O-phosphoethanolamine + H2O PHOSPHO1 exhibits high specific activities toward phosphoethanolamine and phosphocholine, phosphoethanolamine is hydrolyzed 1.5times faster than phosphocholine Homo sapiens ethanolamine + phosphate
-
 ?
3.1.3.75 p-nitrophenyl phosphate + H2O poor substrate Homo sapiens 4-nitrophenol + phosphate
-
 ?
3.1.3.75 phosphocholine + H2O likely a natural substrate, phosphocholine metabolism, PHOSPHO1 is upregulated in mineralizing cells, enzyme is implicated in the generation of phosphate for matrix mineralization Homo sapiens choline + phosphate
-
 ?
3.1.3.75 phosphocholine + H2O PHOSPHO1 exhibits high specific activities toward phosphoethanolamine and phosphocholine, phosphocholine is hydrolyzed 1.5times slower than phosphoethanolamine Homo sapiens choline + phosphate
-
 ?
3.1.3.75 pyridoxal 5'-phosphate + H2O poor substrate Homo sapiens pyridoxal + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.75 ? x * 32000, recombinant PHOSPHO1, SDS-PAGE, Western blot analysis Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.1.3.75 3X11A
-
 Homo sapiens
3.1.3.75 More PHOSPHO1 is a member of the haloacid dehalogenase superfamily of Mg2+-dependent hydrolases Homo sapiens
3.1.3.75 PHOSPHO1
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.75 37
-
 assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.75 1.98
-
 phosphocholine pH 6.7, 37°C, 2 mM Mg2+ Homo sapiens
3.1.3.75 2.27
-
 O-Phosphoethanolamine pH 6.7, 37°C, 2 mM Mg2+ Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.75 6.7
-
 around Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.75 6 7.5 above 70% of maximum activity between pH 6 and 7.2, activity with phosphocholine declines significantly above pH 7.2, activity with phosphoethanolamine declines above pH 7.5 Homo sapiens